Influence of isoleucine upon quinomycin biosynthesis by Streptomyces sp. 732.

Abstract:

:When dl-isoleucine was added to an ammonium nitrate-maltose medium during cultivation of Streptomyces sp. 732, quinomycin B formation was selectively enhanced (from 3 to 70% of the quinomycin mixture) and quinomycin C synthesis was inhibited completely. In addition, two new quinomycins, designated quinomycins D and E, were produced in the presence of isoleucine. These compounds were found to contain N-methylalloisoleucine. In experiments with isoleucine enantiomorphs, it was determined that the order of effectiveness for quinomycin B synthesis was dl-isoleucine > d-isoleucine > l-isoleucine. The extent to which quinomycin B synthesis is enhanced depends upon the concentration and the time of addition of isoleucine to the medium. The effect of dl-isoleucine was reduced to some extent by the addition of l-valine. It is conceivable that amino acids which are precursors of the N-methylamino acids in quinomycin can regulate quinomycin formation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yoshida T,Katagiri K

doi

10.1128/JB.93.4.1327-1331.1967

subject

Has Abstract

pub_date

1967-04-01 00:00:00

pages

1327-31

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

93

pub_type

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