Abstract:
:Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolished when cycloheximide was added up to 10 hr postinfection. In contrast, puromycin, another inhibitor of protein synthesis, did not suppress antigen production. The basis of this differential effect was the inability of puromycin to inhibit protein synthesis in the cells used. This was shown by the failure of the drug to depress the incorporation of labeled amino acid into protein and also failure to inhibit poliovirus synthesis. The puromycin preparation used was very effective in inhibiting poliovirus synthesis in HeLa cells. Thus, appearance of the SV40 T antigen is dependent on protein synthesis in infected cells.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gilden RV,Carp RIdoi
10.1128/JB.91.3.1295-1297.1966subject
Has Abstractpub_date
1966-03-01 00:00:00pages
1295-7issue
3eissn
0021-9193issn
1098-5530journal_volume
91pub_type
杂志文章abstract::Bdellovibrio bacteriovorus is an obligate predatory bacterium that invades and kills a broad range of Gram-negative prey cells, including human pathogens. Its potential therapeutic application has been the subject of increased research interest in recent years. However, an improved understanding of the fundamental mol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00150-20
更新日期:2020-08-25 00:00:00
abstract::We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.3.781-790.1999
更新日期:1999-02-01 00:00:00
abstract::Studies on the composition of total fatty acids of Alcaligenes faecalis harvested at different growth phases have been carried out. Ability of the organism to desaturate palmitic and stearic acid has also been tested. The organism contained palmitic (16:0), stearic (18:0), palmitoleic (16:1), cis-vaccenic (18:1), cycl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.618-624.1973
更新日期:1973-05-01 00:00:00
abstract::The cell surfaces of several Lactobacillus species are covered by a regular layer composed of a single species of protein, the S-protein. The 43-kDa S-protein of the neotype strain Lactobacillus acidophilus ATCC 4356, which originated from the pharynx of a human, was purified. Antibodies generated against purified S-p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6089-6096.1993
更新日期:1993-10-01 00:00:00
abstract::Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.577-581.1963
更新日期:1963-09-01 00:00:00
abstract::Expression of the catalase-peroxidase of Caulobacter crescentus, a gram-negative member of the alpha subdivision of the Proteobacteria, is 50-fold higher in stationary-phase cultures than in exponential cultures. To identify regulators of the starvation response, Tn5 insertion mutants were isolated with reduced expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6152-6159.1999
更新日期:1999-10-01 00:00:00
abstract::The wild strain of Serratia marcescens rapidly degraded threonine and formed aminoacetone in a medium containing glucose and urea. Extracts of this strain showed high threonine dehydrogenase and "biosynthetic" threonine deaminase activities, but no threonine aldolase activity. Threonine dehydrogenase-deficient strain ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.318-323.1978
更新日期:1978-08-01 00:00:00
abstract::Mu promoters active during the lytic cycle were located by isolating RNA at various times after induction of Mu prophages, radiolabeling it by capping in vitro, and hybridizing it to Mu DNA fragments on Southern blots. Signals were detected from four new promoters in addition to the previously characterized Pe (early)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3440-3448.1989
更新日期:1989-06-01 00:00:00
abstract::Group I introns are inserted into genes of a wide variety of bacteriophages of gram-positive bacteria. However, among the phages of enteric and other gram-negative proteobacteria, introns have been encountered only in phage T4 and several of its close relatives. Here we report the insertion of a self-splicing group I ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.8153-8155.2004
更新日期:2004-12-01 00:00:00
abstract::Tetracycline resistance of three Bacteroides fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. Tetracycline resistance markers could be transferred to another B. fragilis strain by filter mating. The transferability was inducible by subinhibitory concentrations of tetracycline...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.141-147.1982
更新日期:1982-04-01 00:00:00
abstract::Flagellate bacteria such as Escherichia coli and Salmonella enterica serovar Typhimurium typically express 5 to 12 flagellar filaments over their cell surface that rotate in clockwise (CW) and counterclockwise directions. These bacteria modulate their swimming direction towards favorable environments by biasing the di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.5.1695-1701.2005
更新日期:2005-03-01 00:00:00
abstract::Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.623-630.1977
更新日期:1977-08-01 00:00:00
abstract::The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.845-857.1980
更新日期:1980-02-01 00:00:00
abstract::It has been clear for over sixty years that the principal method whereby cells replicate and segregate their DNA is semiconservative. It is much less clear why it should be like this rather than, say, conservative. Recently, evidence has accumulated that supports the hypothesis that one of the functions of the cell cy...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00119-19
更新日期:2019-05-22 00:00:00
abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1287-1293.1962
更新日期:1962-06-01 00:00:00
abstract::Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.4951-4958.2000
更新日期:2000-09-01 00:00:00
abstract::Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01748-07
更新日期:2008-02-01 00:00:00
abstract::A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.430-437.1970
更新日期:1970-05-01 00:00:00
abstract::Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.764-780.1994
更新日期:1994-02-01 00:00:00
abstract::Here, we report the draft genome sequence of Antarctic sea ice bacterium Glaciecola punicea ACAM 611(T), the type species of the genus Glaciecola. A blue-light-absorbing proteorhodopsin gene is present in the 3.08-Mb genome. This genome sequence can facilitate the study of the physiological metabolisms and ecological ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00463-12
更新日期:2012-06-01 00:00:00
abstract::Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2031-2034.1968
更新日期:1968-06-01 00:00:00
abstract::Rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. An effective live attenuated vaccine would be extremely useful in the prevention of R. equi disease in horses. Toward that end, we have developed an efficient transposon mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.8.2644-2652.2003
更新日期:2003-04-01 00:00:00
abstract::The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3783-3785.1997
更新日期:1997-06-01 00:00:00
abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.153-160.1979
更新日期:1979-01-01 00:00:00
abstract::DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2652-2661.1993
更新日期:1993-05-01 00:00:00
abstract::Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00304-20
更新日期:2020-11-04 00:00:00
abstract::Glucose-6-phosphate dehydrogenase was partially purified from both glucose-grown and iron-glucose-grown Thiobacillus ferrooxidans. The enzyme possesses a dual nucleotide specificity for either nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) and has a molecular weight of 11...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.343-352.1971
更新日期:1971-10-01 00:00:00
abstract::Weaver, Judith R. (Iowa State University, Ames), and P. A. Pattee. Inducible resistance to erythromycin in Staphylococcus aureus. J. Bacteriol. 88:574-580. 1964.-The dissociated resistance of Staphylococcus aureus to erythromycin was examined and was found to possess the characteristics of an inducible enzyme. The ind...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.574-580.1964
更新日期:1964-09-01 00:00:00
abstract::Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.895-900.1963
更新日期:1963-04-01 00:00:00
abstract::Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.2.231-235.1998
更新日期:1998-01-01 00:00:00