Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.

Abstract:

:Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolished when cycloheximide was added up to 10 hr postinfection. In contrast, puromycin, another inhibitor of protein synthesis, did not suppress antigen production. The basis of this differential effect was the inability of puromycin to inhibit protein synthesis in the cells used. This was shown by the failure of the drug to depress the incorporation of labeled amino acid into protein and also failure to inhibit poliovirus synthesis. The puromycin preparation used was very effective in inhibiting poliovirus synthesis in HeLa cells. Thus, appearance of the SV40 T antigen is dependent on protein synthesis in infected cells.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gilden RV,Carp RI

doi

10.1128/JB.91.3.1295-1297.1966

subject

Has Abstract

pub_date

1966-03-01 00:00:00

pages

1295-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

91

pub_type

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