Abstract:
:Total DNA of various Rhizobium galegae strains representing different geographical origins, and taxonomic divergence was digested with three restriction enzymes separately, Southern blotted, and hybridized with six heterologous probes. The sequence divergences for different pairwise comparisons were calculated from proportions of conserved hybridizing fragments. The unweighted pair group method was used to group the strains. The symbiotic common nod and nifHDK probes used were highly conserved and grouped the strains according to the host plant, Galega orientalis or G. officinalis. The grouping derived from combined data of the constitutive hemA, glnA, ntrC, and recA probes was similar to that obtained in total DNA-DNA hybridization experiments. The constitutive probes grouped the strains in a different order than did the symbiotic probes, a result that may reflect interstrain transfer of symbiotic sequences in the course of evolution.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kaijalainen S,Lindström Kdoi
10.1128/jb.171.10.5561-5566.1989subject
Has Abstractpub_date
1989-10-01 00:00:00pages
5561-6issue
10eissn
0021-9193issn
1098-5530journal_volume
171pub_type
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