Primary structure of cyanelle peptidoglycan of Cyanophora paradoxa: a prokaryotic cell wall as part of an organelle envelope.

Abstract:

:The peptidoglycan layer surrounding the photosynthetic organelles (cyanelles) of the protist Cyanophora paradoxa is thought to be a relic of their cyanobacterial ancestors. The separation of muropeptides by gel filtration and reverse-phase high-performance liquid chromatography revealed four different muropeptide monomers. A number of muropeptides were identical in retention behavior to muropeptides of Escherichia coli, while others had remarkably long retention times with respect to their sizes, as indicated by gel filtration. Molecular mass determination by plasma desorption and matrix-assisted laser desorption ionization mass spectrometry showed that these unusual muropeptides had molecular masses greater by 112 Da or a multiple thereof than those of ones common to both species. Fast atom bombardment-tandem mass spectrometry of these reduced muropeptide monomers allowed the localization of the modification to D-glutamic acid. High-resolution fast atom bombardment-mass spectrometry and amino acid analysis revealed N-acetylputrescine to be the substituent (E. Pittenauer, E. R. Schmid, G. Allmaier, B. Pfanzagl, W. Löffelhardt, C. Quintela, M. A. de Pedro, and W. Stanek, Biol. Mass Spectrom. 22:524-536, 1993). In addition to the 4 monomers already known, 8 dimers, 11 trimers, and 6 tetramers were characterized. An average glycan chain length of 51 disaccharide units was determined by the transfer of [U-14C]galactose to the terminal N-acetylglucosamine residues of cyanelle peptidoglycan. The muropeptide pattern is discussed with respect to peptidoglycan biosynthesis and processing.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pfanzagl B,Zenker A,Pittenauer E,Allmaier G,Martinez-Torrecuadrada J,Schmid ER,De Pedro MA,Löffelhardt W

doi

10.1128/jb.178.2.332-339.1996

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

332-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Complementation of the Fo c subunit of Escherichia coli with that of Streptococcus mutans and properties of the hybrid FoF1 ATP synthase.

    abstract::The c subunit of Streptococcus mutans ATP synthase (FoF1) is functionally exchangeable with that of Escherichia coli, since E. coli with a hybrid FoF1 is able to grow on minimum succinate medium through oxidative phosphorylation. E. coli F1 bound to the hybrid Fo with the S. mutans c subunit showed N,N'-dicyclohexylca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00542-13

    authors: Araki M,Hoshi K,Fujiwara M,Sasaki Y,Yonezawa H,Senpuku H,Iwamoto-Kihara A,Maeda M

    更新日期:2013-11-01 00:00:00

  • Complete genome sequence of the anaerobic, protein-degrading hyperthermophilic crenarchaeon Desulfurococcus kamchatkensis.

    abstract::Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01525-08

    authors: Ravin NV,Mardanov AV,Beletsky AV,Kublanov IV,Kolganova TV,Lebedinsky AV,Chernyh NA,Bonch-Osmolovskaya EA,Skryabin KG

    更新日期:2009-04-01 00:00:00

  • The elastic properties of the caulobacter crescentus adhesive holdfast are dependent on oligomers of N-acetylglucosamine.

    abstract::The aquatic bacterium Caulobacter crescentus attaches to solid surfaces through an adhesive holdfast located at the tip of its polar stalk, a thin cylindrical extension of the cell membrane. In this paper, the elastic properties of the C. crescentus stalk and holdfast assembly were studied by using video light microsc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.257-265.2005

    authors: Li G,Smith CS,Brun YV,Tang JX

    更新日期:2005-01-01 00:00:00

  • Importance of anaerobic superoxide dismutase synthesis in facilitating outgrowth of Escherichia coli upon entry into an aerobic habitat.

    abstract::The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.24.7653-7658.1994

    authors: Kargalioglu Y,Imlay JA

    更新日期:1994-12-01 00:00:00

  • Role of EscP (Orf16) in injectisome biogenesis and regulation of type III protein secretion in enteropathogenic Escherichia coli.

    abstract::Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01215-12

    authors: Monjarás Feria J,García-Gómez E,Espinosa N,Minamino T,Namba K,González-Pedrajo B

    更新日期:2012-11-01 00:00:00

  • Regulation of the Salmonella typhimurium aroF gene in Escherichia coli.

    abstract::The Salmonella typhimurium aroF gene, encoding the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, was localized to a chromosomal PstI fragment by Southern blotting with an Escherichia coli aroF probe. This fragment was cloned by screening a plasmid library for complementation of an E. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2259-2266.1990

    authors: Muday GK,Herrmann KM

    更新日期:1990-05-01 00:00:00

  • Chemotaxis toward amino acids in Escherichia coli.

    abstract::Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.315-326.1972

    authors: Mesibov R,Adler J

    更新日期:1972-10-01 00:00:00

  • Tungsten and molybdenum regulation of formate dehydrogenase expression in Desulfovibrio vulgaris Hildenborough.

    abstract::Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00042-11

    authors: da Silva SM,Pimentel C,Valente FM,Rodrigues-Pousada C,Pereira IA

    更新日期:2011-06-01 00:00:00

  • Probing the structure, function, and interactions of the Escherichia coli H-NS and StpA proteins by using dominant negative derivatives.

    abstract::Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.15.4335-4343.1996

    authors: Williams RM,Rimsky S,Buc H

    更新日期:1996-08-01 00:00:00

  • Production of large amounts of acetate during germination of Bacillus megaterium spores in the absence of exogenous carbon sources.

    abstract::When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.744-746.1977

    authors: Setlow B,Shay LK,Vary JC,Setlow P

    更新日期:1977-11-01 00:00:00

  • Regions of Salmonella typhimurium flagellin essential for its polymerization and excretion.

    abstract::Immunological methods were used to examine the flagellin production of Salmonella typhimurium strains that carried a mutation in one of the two possible genes for flagellin (H1 or H2) and also were incapable of expressing the other gene. Some mutants produced flagellin that was excreted into the culture medium; others...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.291-296.1987

    authors: Homma M,Fujita H,Yamaguchi S,Iino T

    更新日期:1987-01-01 00:00:00

  • Regulation and function of Escherichia coli sugar efflux transporter A (SetA) during glucose-phosphate stress.

    abstract::Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01008-10

    authors: Sun Y,Vanderpool CK

    更新日期:2011-01-01 00:00:00

  • Menaquinone (vitamin K2) biosynthesis: localization and characterization of the menA gene from Escherichia coli.

    abstract::A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1, 4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.10.2782-2787.1998

    authors: Suvarna K,Stevenson D,Meganathan R,Hudspeth ME

    更新日期:1998-05-01 00:00:00

  • Cytosine methylation of the sequence GATC in a mycoplasma.

    abstract::Mycoplasma virus L2 is subject to host-specific restriction and modification in Acholeplasma laidlawii strains JA1 and K2. We have examined the DNAs from both host cells and viruses propagated on these strains with respect to susceptibility to cleavage by restriction endonucleases and for DNA base modifications. We sh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1420-1424.1982

    authors: Dybvig K,Swinton D,Maniloff J,Hattman S

    更新日期:1982-09-01 00:00:00

  • Convenient transduction of recA with bacteriophage T4GT7.

    abstract::The generalized transducing phage T4GT7 grew well on recA strains of Escherichia coli and transduced recA into F- and Hfr strains of E. coli at high frequency. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.3.1072-1073.1984

    authors: Plakidou S,Moffat KG,Salmond GP,Mackinnon G

    更新日期:1984-09-01 00:00:00

  • Identification of a target gene and activating stimulus for the YpdA/YpdB histidine kinase/response regulator system in Escherichia coli.

    abstract::Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02051-12

    authors: Fried L,Behr S,Jung K

    更新日期:2013-02-01 00:00:00

  • How many initiator tRNA genes does Escherichia coli need?

    abstract::Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01620-14

    authors: Samhita L,Nanjundiah V,Varshney U

    更新日期:2014-07-01 00:00:00

  • Isolation and characterization of flagella and flagellin proteins from the Thermoacidophilic archaea Thermoplasma volcanium and Sulfolobus shibatae.

    abstract::Isolated flagellar filaments of Sulfolobus shibatae were 15 nm in diameter, and they were composed of two major flagellins which have M(r)s of 31,000 and 33,000 and which stained positively for glycoprotein. The flagellar filaments of Thermoplasma volcanium were 12 nm in diameter and were composed of one major flagell...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.3.902-905.1996

    authors: Faguy DM,Bayley DP,Kostyukova AS,Thomas NA,Jarrell KF

    更新日期:1996-02-01 00:00:00

  • Isolation and properties of the complex between the enhancer binding protein NIFA and the sensor NIFL.

    abstract::In Azotobacter vinelandii, activation of nif gene expression by the transcriptional regulatory enhancer binding protein NIFA is controlled by the sensor protein NIFL in response to changes in levels of oxygen and fixed nitrogen in vivo. NIFL is a novel redox-sensing flavoprotein which is also responsive to adenosine n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.15.4461-4468.1999

    authors: Money T,Jones T,Dixon R,Austin S

    更新日期:1999-08-01 00:00:00

  • Deoxyribonucleic acid synthesis in Saccharomyces cerevisiae cells permeabilized with ether.

    abstract::Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.333-341.1979

    authors: Oertel W,Goulian M

    更新日期:1979-11-01 00:00:00

  • ftsZ is an essential cell division gene in Escherichia coli.

    abstract::The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect. This strain was temperature sensitive for cell division and viability, confirming that ft...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.11.3500-3506.1991

    authors: Dai K,Lutkenhaus J

    更新日期:1991-06-01 00:00:00

  • Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis.

    abstract::Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.782-796.1985

    authors: Shaw JH,Clewell DB

    更新日期:1985-11-01 00:00:00

  • Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.

    abstract::A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.16.4794-4800.1996

    authors: Morbidoni HR,de Mendoza D,Cronan JE Jr

    更新日期:1996-08-01 00:00:00

  • Collaborative regulation of Escherichia coli glutamate-dependent acid resistance by two AraC-like regulators, GadX and GadW (YhiW).

    abstract::An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.24.7001-7012.2002

    authors: Ma Z,Richard H,Tucker DL,Conway T,Foster JW

    更新日期:2002-12-01 00:00:00

  • Presence of polyribosomes in condiospores of Botryodiplodia theobromae harvested with nonaqueous solvents.

    abstract::Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.1394-1395.1975

    authors: Brambl R

    更新日期:1975-06-01 00:00:00

  • Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2.

    abstract::The complete nucleotide sequence of the linear plasmid pBD2 from Rhodococcus erythropolis BD2 comprises 210,205 bp. Sequence analyses of pBD2 revealed 212 putative open reading frames (ORFs), 97 of which had an annotatable function. These ORFs could be assigned to six functional groups: plasmid replication and mainten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.17.5269-5274.2003

    authors: Stecker C,Johann A,Herzberg C,Averhoff B,Gottschalk G

    更新日期:2003-09-01 00:00:00

  • DnaK mutants defective in ATPase activity are defective in negative regulation of the heat shock response: expression of mutant DnaK proteins results in filamentation.

    abstract::Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.764-780.1994

    authors: McCarty JS,Walker GC

    更新日期:1994-02-01 00:00:00

  • Inactivation of protein translocation by cold-sensitive mutations in the yajC-secDF operon.

    abstract::Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.19.6852-6855.2005

    authors: Nouwen N,Driessen AJ

    更新日期:2005-10-01 00:00:00

  • Comparison of the activities of extracts of Escherichia coli and Salmonella typhimurium in amino acid incorporation.

    abstract::We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts transla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.757-763.1973

    authors: Bassel BA,Curry ME

    更新日期:1973-11-01 00:00:00

  • VIRULENCE-LINKED COLONIAL AND MORPHOLOGICAL VARIATION IN LEPTOSPIRA.

    abstract::Faine, S. (University of Sydney, Sydney, Australia), and J. van der Hoeden. Virulence-linked colonial and morphological variation in Leptospira. J. Bacteriol. 88:1493-1496. 1964.-Large-colony typical hooked Leptospira icterohaemorrhagiae was virulent for hamsters and guinea pigs. On cultivation, it was gradually repla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1493-1496.1964

    authors: FAINE S,VANDERHOEDEN J

    更新日期:1964-11-01 00:00:00