Deoxyribonucleic acid synthesis in Saccharomyces cerevisiae cells permeabilized with ether.

abstract：:We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.88) of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...

journal_title：Journal of bacteriology

authors： Wang YL,Beach MJ,Rodwell VW

更新日期：1989-10-01 00:00:00

abstract：:The ability to reduce Hg(II) to Hg(0), which is determined by a plasmid-borne gene in Escherichia coli, is conferred by a Hg(II)-inducible activity which is located in the cytoplasm rather than in the periplasmic space of the cell. This Hg(II)-reducing activity can be isolated from the supernatant of a 160,000 x g cen...

journal_title：Journal of bacteriology

authors： Summers AO,Sugarman LI

更新日期：1974-07-01 00:00:00

abstract：:An S-adenosylhomocysteine deaminase has been isolated and purified from streptonigrin-producing Streptomyces flocculus ATCC 13257. Deamination represents the major metabolic route of S-adenosylhomocysteine in this organism. The protein was found to be monomeric with a molecular weight of 56,100 +/- 1,600. The activity...

journal_title：Journal of bacteriology

authors： Zulty JJ,Speedie MK

更新日期：1989-12-01 00:00:00

abstract：:Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, during vegetative growth. The CwlF protein was extracted from B. subtilis cwlB sigD mutant cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequencing revealed that its sequence is completely identical...

journal_title：Journal of bacteriology

authors： Ishikawa S,Hara Y,Ohnishi R,Sekiguchi J

更新日期：1998-05-01 00:00:00

abstract：:Alginates are polysaccharides composed of 1-4-linked beta-D-mannuronic acid and alpha-L-guluronic acid. The polymer can be degraded by alginate lyases, which cleave the polysaccharide using a beta-elimination reaction. Two such lyases have previously been identified in the soil bacterium Azotobacter vinelandii, as fol...

journal_title：Journal of bacteriology

authors： Gimmestad M,Ertesvåg H,Heggeset TM,Aarstad O,Svanem BI,Valla S

更新日期：2009-08-01 00:00:00

abstract：:Previous work has shown that lethal heat treatment of Bacillus subtilis spores lacking the major DNA-binding proteins SASP-alpha and -beta (alpha-beta- spores) causes significant DNA damage, including many single-strand breaks. In this work we have used a reagent specific for aldehydes present in abasic sites in DNA t...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1994-04-01 00:00:00

abstract：:The inability of Micrococcus sodonensis to grow on glucose as the sole source of carbon and energy was investigated. Estimation of pathways of glucose catabolism indicated that both the glycolytic and hexose monophosphate pathways are present in this organism. Comparative studies with Escherichia coli demonstrated tha...

journal_title：Journal of bacteriology

authors： Perry JJ,Evans JB

更新日期：1967-06-01 00:00:00

abstract：:Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

journal_title：Journal of bacteriology

authors： MacGregor CH,Schnaitman CA

更新日期：1971-10-01 00:00:00

abstract：:Holwerda, Jack (Michigan Department of Health, Grand Rapids) and Grace Eldering. Culture and fluorescent-antibody methods in diagnosis of whooping cough. J. Bacteriol. 86:449-451. 1963.-Nasopharyngeal swabs from 517 suspected whooping-cough patients were examined by culture and by fluorescent-antibody (FA) staining pr...

journal_title：Journal of bacteriology

authors： HOLWERDA J,ELDERING G

更新日期：1963-09-01 00:00:00

abstract：:DNA helicase I, encoded on the Escherichia coli F plasmid, catalyzes a site- and strand-specific nicking reaction within the F plasmid origin of transfer (oriT) to initiate conjugative DNA strand transfer. The product of the nicking reaction contains a single phosphodiester bond interruption as determined by single-nu...

journal_title：Journal of bacteriology

authors： Matson SW,Nelson WC,Morton BS

更新日期：1993-05-01 00:00:00

abstract：:At a pH of

journal_title：Journal of bacteriology

authors： Azarkina N,Konstantinov AA

更新日期：2002-10-01 00:00:00

abstract：:Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...

journal_title：Journal of bacteriology

authors： Frias MJ,Melo-Cristino J,Ramirez M

更新日期：2009-09-01 00:00:00

abstract：:Division site placement in Escherichia coli involves interactions of the MinD protein with MinC and MinE and with other MinD molecules to form membrane-associated polymeric structures. In this work, as part of a study of these interactions, we established that heterologous membrane-associated proteins such as MinD can...

journal_title：Journal of bacteriology

authors： Taghbalout A,Ma L,Rothfield L

更新日期：2006-04-01 00:00:00

abstract：:Biberstein, Ernst L. (University of California, Davis) and Patricia D. Spencer. Oxidative metabolism of Haemophilus species grown at different levels of hemin supplementation. J. Bacteriol. 84:916-920. 1962.-Haemophilus strains were propagated on hemin-deficient and hemin-supplemented media and tested on various subst...

journal_title：Journal of bacteriology

authors： BIBERSTEIN EL,SPENCER PD

更新日期：1962-11-01 00:00:00

abstract：:The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence whic...

journal_title：Journal of bacteriology

authors： Hayashi H,Takagi KI,Fukumura M,Kimura T,Karita S,Sakka K,Ohmiya K

更新日期：1997-07-01 00:00:00

abstract：:The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...

journal_title：Journal of bacteriology

authors： Trinh S,Reysset G

更新日期：1997-06-01 00:00:00

abstract：:Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography...

journal_title：Journal of bacteriology

authors： Hong SB,Farrand SK

更新日期：1996-04-01 00:00:00

abstract：:Mycoplasma ovipneumoniae belongs to Mycoplasma, a genus containing the smallest self-replicating microorganisms, and causes infectious pleuropneumonia in goats and sheep. Nucleotide-binding oligomerization domain-containing protein (NOD2), an intracellular pattern recognition receptor, interacts with muramyl dipeptide...

journal_title：Journal of bacteriology

authors： Luo H,Wu X,Xu Z,Hao X,Wang Y,Li M

更新日期：2020-09-23 00:00:00

abstract：:A new insertion element, IS1549, was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isola...

journal_title：Journal of bacteriology

authors： Plikaytis BB,Crawford JT,Shinnick TM

更新日期：1998-03-01 00:00:00

abstract：:Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1995-10-01 00:00:00

abstract：:Dienes, Louis (Massachusetts General Hospital, Boston, Mass.), Bojana M. Bandur, and Sarabelle Madoff. Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bacteriol. 87:1471-1476. 1964.-Strains of Neisseria gonorrhoeae (22) were studied immediately after isolation from patients. After 24 to 48 hr of inc...

journal_title：Journal of bacteriology

authors： DIENES L,BANDUR BM,MADOFF S

更新日期：1964-06-01 00:00:00

abstract：:SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome si...

journal_title：Journal of bacteriology

authors： Maiques E,Ubeda C,Tormo MA,Ferrer MD,Lasa I,Novick RP,Penadés JR

更新日期：2007-08-01 00:00:00

abstract：:Germination of Bacillus spores is triggered by the binding of specific nutrients to germinant receptors (GRs) located in the spore's inner membrane. The GRs typically consist of A, B, and C subunits, encoded by tricistronic ger operons. The Bacillus licheniformis genome contains the gerA family operons gerA, ynd, and ...

journal_title：Journal of bacteriology

authors： Aspholm M,Borch-Pedersen K,O'Sullivan K,Fjellheim S,Aardal IB,Granum PE,Lindbäck T

更新日期：2019-10-04 00:00:00

abstract：:In contrast to Escherichia coli and Salmonella typhimurium, Bacillus subtilis could convert ethionine to S-adenosylethionine (SAE), as can Saccharomyces cerevisiae. This conversion was essential for growth inhibition by ethionine because metE mutants which were deficient in S-adenosylmethionine synthetase activity, we...

journal_title：Journal of bacteriology

authors： Allen ER,Orrego C,Wabiko H,Freese E

更新日期：1986-04-01 00:00:00

abstract：:The S-layers of Thermus thermophilus HB27 and T. thermophilus HB8 are composed of protein units of 95 kDa (P95) and 100 kDa (P100), respectively. We have selected S-layer deletion mutants from both strains by complete replacement of the slpA gene. Mutants of the two strains showed similar defects in growth and morphol...

journal_title：Journal of bacteriology

authors： Fernández-Herrero LA,Olabarría G,Castón JR,Lasa I,Berenguer J

更新日期：1995-10-01 00:00:00

abstract：:A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosi...

journal_title：Journal of bacteriology

authors： Hattman S,Schlagman S,Cousens L

更新日期：1973-09-01 00:00:00

abstract：:This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...

journal_title：Journal of bacteriology

authors： Vakharia H,German GJ,Misra R

更新日期：2001-12-01 00:00:00

abstract：:Escherichia coli suffered 95 to 100% lethality when exposed to 1.0 mM paraquat for 30 min at 37 degrees C in aerobic nutrient broth medium but did not lose viability when the exposure was done in Vogel Bonner or tryptic soy yeast extract medium. Paraquat was, however, bacteriostatic in all of these media. Salts, added...

journal_title：Journal of bacteriology

authors： Kitzler J,Fridovich I

更新日期：1986-07-01 00:00:00

abstract：:Small plasmids which replicate in both Escherichia coli and Clostridium perfringens were made by recombining E. coli plasmid pBR322 with three different small (less than 4 kilobases) plasmids native to C. perfringens. Subsequently, two homologous, though distinct, tetracycline resistance determinants (tet) from other ...

journal_title：Journal of bacteriology

authors： Squires CH,Heefner DL,Evans RJ,Kopp BJ,Yarus MJ

更新日期：1984-08-01 00:00:00

abstract：:A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS functions in rdgB mutants at the nonpermissive temperature. We identified the cloned gene as purA. ...

journal_title：Journal of bacteriology

authors： Clyman J,Cunningham RP

更新日期：1991-02-01 00:00:00