Heat inactivation of Bacillus subtilis spores lacking small, acid-soluble spore proteins is accompanied by generation of abasic sites in spore DNA.

abstract：:A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...

journal_title：Journal of bacteriology

authors： Cahn FH,Fox MS

更新日期：1968-03-01 00:00:00

abstract：:Reticulate bodies of the meningopneumonitis (MP) microorganism were purified from L cells 18 hr after infection by the combination of differential centrifugation in 30% sucrose solution and potassium tartrate density gradient centrifugation. It was ascertained by electron microscopy that purified preparations of retic...

journal_title：Journal of bacteriology

authors： Tamura A,Matsumoto A,Higashi N

更新日期：1967-06-01 00:00:00

abstract：:The O polysaccharide of the lipopolysaccharide (O antigen) of Gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given O-antigen type, thus supporting the concept of the adaptive significance of the O-antigen variability in bacteria. The O-antigen layer also mod...

journal_title：Journal of bacteriology

authors： Knirel YA,Prokhorov NS,Shashkov AS,Ovchinnikova OG,Zdorovenko EL,Liu B,Kostryukova ES,Larin AK,Golomidova AK,Letarov AV

更新日期：2015-03-01 00:00:00

abstract：:Spectral analysis indicated the presence of a cytochrome cbb3 oxidase under microaerobic conditions in Azospirillum brasilense Sp7 cells. The corresponding genes (cytNOQP) were isolated by using PCR. These genes are organized in an operon, preceded by a putative anaerobox. The phenotype of an A. brasilense cytN mutant...

journal_title：Journal of bacteriology

authors： Marchal K,Sun J,Keijers V,Haaker H,Vanderleyden J

更新日期：1998-11-01 00:00:00

abstract：:The general aromatic amino acid permease, AroP, of Escherichia coli is responsible for the active transport of phenylalanine, tyrosine, and tryptophan. A proposed topological model for the AroP permease, consisting of 12 hydrophobic transmembrane spans connected by hydrophilic loops, is very similar to that of the clo...

journal_title：Journal of bacteriology

authors： Cosgriff AJ,Pittard AJ

更新日期：1997-05-01 00:00:00

abstract：:Many Escherichia coli K-12 strains display an intrinsic resistance to the peptide antibiotic microcin J25. In this study, we present results showing that the leucine-responsive regulatory protein, Lrp, is involved in this phenotype by acting as a positive regulator of YojI, a chromosomally encoded efflux pump which ex...

journal_title：Journal of bacteriology

authors： Socías SB,Vincent PA,Salomón RA

更新日期：2009-02-01 00:00:00

abstract：:The ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobU in Salmonella enterica). The latter activity is required for cobinamide salvaging in bacteria. This paper reports evidence that ar...

journal_title：Journal of bacteriology

authors： Woodson JD,Zayas CL,Escalante-Semerena JC

更新日期：2003-12-01 00:00:00

abstract：:Biberstein, Ernst L. (University of California, Davis) and Patricia D. Spencer. Oxidative metabolism of Haemophilus species grown at different levels of hemin supplementation. J. Bacteriol. 84:916-920. 1962.-Haemophilus strains were propagated on hemin-deficient and hemin-supplemented media and tested on various subst...

journal_title：Journal of bacteriology

authors： BIBERSTEIN EL,SPENCER PD

更新日期：1962-11-01 00:00:00

abstract：:The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highl...

journal_title：Journal of bacteriology

authors： Vonder Haar RA,Legrain M,Kolbe HV,Jacobs E

更新日期：1994-10-01 00:00:00

abstract：:Ramakrishnan, T. (Yale University, New Haven, Conn.), and Edward A. Adelberg. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J. Bacteriol. 89:654-660. 1965.-A tightly clustered set of five structural genes governs the synthesis of the five enzymes of isole...

journal_title：Journal of bacteriology

authors： RAMAKRISHNAN T,ADELBERG EA

更新日期：1965-03-01 00:00:00

abstract：:Entry into intestinal epithelial cells is an essential step in the pathogenesis of Salmonella infections. Our laboratory has previously identified a genetic locus, inv, that is necessary for efficient entry of Salmonella typhimurium into cultured epithelial cells. We have carried out a molecular and functional analysi...

journal_title：Journal of bacteriology

authors： Eichelberg K,Ginocchio CC,Galán JE

更新日期：1994-08-01 00:00:00

abstract：:A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...

journal_title：Journal of bacteriology

authors： Dabbs ER

更新日期：1980-11-01 00:00:00

abstract：:A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

journal_title：Journal of bacteriology

authors： Kelly R,Kwon-Chung KJ

更新日期：1992-01-01 00:00:00

abstract：:The DNA-dependent RNA polymerase (EC 2.7.7.6) from Clostridium acetobutylicum DSM 1731 has been purified to homogeneity and characterized. The purified enzyme was composed of four subunits and had a molecular mass of 370,000 Da. Western immunoblot analysis with polyclonal antibodies against the sigma 70 subunit of Esc...

journal_title：Journal of bacteriology

authors： Pich A,Bahl H

更新日期：1991-03-01 00:00:00

abstract：:In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expr...

journal_title：Journal of bacteriology

authors： Hall JA,Pajor AM

更新日期：2007-02-01 00:00:00

abstract：:The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...

journal_title：Journal of bacteriology

authors： Kargalioglu Y,Imlay JA

更新日期：1994-12-01 00:00:00

abstract：:Davies, Helen C. (University of Pennsylvania, Philadelphia), Fred Karush, and Joanne H. Rudd. Effect of amino acids on steady-state growth of a group A hemolytic streptococcus. J. Bacteriol. 89:421-427. 1965.-A study has been made of amino acid utilization by a strain of type 4, group A streptococcus growing under ste...

journal_title：Journal of bacteriology

authors： Davies HC,Karush F,Rudd JH

更新日期：1965-02-01 00:00:00

abstract：:MukBEF is a bacterial SMC (structural maintenance of chromosome) complex required for chromosome partitioning in Escherichia coli. We report that overproduction of MukBEF results in marked chromosome condensation. This condensation is rapid and precedes the effects of overproduction on macromolecular synthesis. Conden...

journal_title：Journal of bacteriology

authors： Wang Q,Mordukhova EA,Edwards AL,Rybenkov VV

更新日期：2006-06-01 00:00:00

abstract：:The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl ...

journal_title：Journal of bacteriology

authors： Maier E,Polleichtner G,Boeck B,Schinzel R,Benz R

更新日期：2001-01-01 00:00:00

abstract：:Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...

journal_title：Journal of bacteriology

authors： Ulmanen I,Lundström K,Lehtovaara P,Sarvas M,Ruohonen M,Palva I

更新日期：1985-04-01 00:00:00

abstract：:We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localiza...

journal_title：Journal of bacteriology

authors： Donald JW,Hicks MG,Richardson DJ,Palmer T

更新日期：2008-07-01 00:00:00

abstract：:An Escherichia coli F19 recA, nitrate reductase-deficient mutant was constructed by transposon mutagenesis and shown to be resistant to metronidazole. This mutant was a most suitable host for the isolation of Clostridium acetobutylicum genes on recombinant plasmids, which activated metronidazole and rendered the E. co...

journal_title：Journal of bacteriology

authors： Santangelo JD,Jones DT,Woods DR

更新日期：1991-02-01 00:00:00

abstract：:The following results with an in vitro replication system utilizing a plasmid RK2 DNA-membrane complex indicate that the essential trfA-encoded replication protein of RK2 is present and active in the complex. (i) A complex extracted from a conditional replication mutant of RK2, which contains a temperature-sensitive m...

journal_title：Journal of bacteriology

authors： Kornacki JA,Firshein W

更新日期：1986-07-01 00:00:00

abstract：:A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology u...

journal_title：Journal of bacteriology

authors： Borgia PT,Gokul NK,Phillips GJ

更新日期：1985-12-01 00:00:00

abstract：:To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...

journal_title：Journal of bacteriology

authors： Hatfull GF,Joyce CM

更新日期：1986-06-01 00:00:00

abstract：:Microorganisms and plants utilize two-component systems to regulate adaptive responses to changing environmental conditions. Sensor kinases detect stimuli and alter their autophosphorylation activity accordingly. Signal propagation occurs via the transfer of phosphoryl groups from upstream kinases to downstream respon...

journal_title：Journal of bacteriology

authors： Straughn PB,Vass LR,Yuan C,Kennedy EN,Foster CA,Bourret RB

更新日期：2020-07-09 00:00:00

abstract：:Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

journal_title：Journal of bacteriology

authors： MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

更新日期：1993-12-01 00:00:00

abstract：:Smith, P. B. (Communicable Disease Center, Atlanta, Ga.). Growth of staphylococci on mercuric chloride agar. J. Bacteriol. 84:1016-1019. 1962.-An attempt has been made to corroborate a report that "epidemic" strains of staphylococci are more resistant to mercuric salts than are "nonepidemic" strains. A comparison of m...

journal_title：Journal of bacteriology

authors： SMITH PB

更新日期：1962-11-01 00:00:00

abstract：:Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be t...

journal_title：Journal of bacteriology

authors： Hill EB,Wayne LG,Gross WM

更新日期：1972-12-01 00:00:00

abstract：:Welker, N. E. (University of Illinois, Urbana), and L. Leon Campbell. Biochemical changes in lysogenic Bacillus stearothermophilus after bacteriophage induction. J. Bacteriol. 90:1129-1137. 1965.-Cultures of Bacillus stearothermophilus 1503-4R (TP-1) continued to grow at an unaltered rate after induction with mitomyci...

journal_title：Journal of bacteriology

authors： Welker NE,Campbell LL

更新日期：1965-10-01 00:00:00