Deletion of the spf (spot 42 RNA) gene of Escherichia coli.

abstract：:The polycistronic mRNA encoding the nine genes of the unc operon of Escherichia coli was studied. We demonstrated the ribosome-binding capabilities of six of the nine unc genes, uncB, uncE, uncF, uncH, uncA, and uncD, by using the technique of primer extension inhibition or "toeprinting." No toeprint was detected for ...

journal_title：Journal of bacteriology

authors： Schaefer EM,Hartz D,Gold L,Simoni RD

更新日期：1989-07-01 00:00:00

abstract：:We showed that the heat killing curve for exponentially growing Saccharomyces cerevisiae was biphasic. This suggests two populations of cells with different thermal killing characteristics. When exponentially growing cells separated into cell cycle-specific fractions via centrifugal elutriation were heat shocked, the ...

journal_title：Journal of bacteriology

authors： Plesset J,Ludwig JR,Cox BS,McLaughlin CS

更新日期：1987-02-01 00:00:00

abstract：:The ability of Rickettsia prowazekii to transport potential sources of the glucose moiety of bacterial polysaccharides was determined. Transport was determined both by filtration assays and by centrifugation through nonaqueous layers. Uridine 5'-diphosphoglucose (UDPG) was transported, whereas glucose was not transpor...

journal_title：Journal of bacteriology

authors： Winkler HH,Daugherty RM

更新日期：1986-09-01 00:00:00

abstract：:Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...

journal_title：Journal of bacteriology

authors： Popham DL,Illades-Aguiar B,Setlow P

更新日期：1995-08-01 00:00:00

abstract：:ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...

journal_title：Journal of bacteriology

authors： Tanaka S,Lee SO,Hamaoka K,Kato J,Takiguchi N,Nakamura K,Ohtake H,Kuroda A

更新日期：2003-09-01 00:00:00

abstract：:Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...

journal_title：Journal of bacteriology

authors： Parish T,Roberts G,Laval F,Schaeffer M,Daffé M,Duncan K

更新日期：2007-05-01 00:00:00

abstract：:The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...

journal_title：Journal of bacteriology

authors： Schlindwein C,Giordano G,Santini CL,Mandrand MA

更新日期：1990-10-01 00:00:00

abstract：:The utilization of glycerol as a carbon source for growth by Klebsiella aerogenes, strain 2103, involves separate aerobic (sn-glycerol-3-phosphate or G3P) and anaerobic (dihydroxyacetone or DHA) pathways of catabolism. Enzyme and transport activities of the aerobic pathway are elevated in cells grown under oxygenated ...

journal_title：Journal of bacteriology

authors： Ruch FE,Lengeler J,Lin EC

更新日期：1974-07-01 00:00:00

abstract：:Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

journal_title：Journal of bacteriology

authors： Brandriss MC

更新日期：1979-06-01 00:00:00

abstract：:Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilizatio...

journal_title：Journal of bacteriology

authors： Joshua CJ,Dahl R,Benke PI,Keasling JD

更新日期：2011-03-01 00:00:00

abstract：:Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...

journal_title：Journal of bacteriology

authors： Smith RV,Evans MC

更新日期：1971-03-01 00:00:00

abstract：:Analysis of a Yersinia pestis Delta caf1A mutant demonstrated that the Caf1A usher is required for the assembly and secretion of the fraction 1 capsule. The capsule assembled into thin fibrils and denser aggregates on the bacterial surface. Pilus-like fibers were also detected on the surface of Y. pestis. The capsule ...

journal_title：Journal of bacteriology

authors： Runco LM,Myrczek S,Bliska JB,Thanassi DG

更新日期：2008-05-01 00:00:00

abstract：:A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription f...

journal_title：Journal of bacteriology

authors： Murray EJ,Strauch MA,Stanley-Wall NR

更新日期：2009-11-01 00:00:00

abstract：:Starvation and cell density regulate the developmental expression of Myxococcus xanthus gene 4521. Three classes of mutants allow expression of this developmental gene during growth on nutrient agar, such that colonies of strains containing a Tn5 lac Omega4521 fusion are Lac(+). One class of these mutants inactivates ...

journal_title：Journal of bacteriology

authors： Guo D,Wu Y,Kaplan HB

更新日期：2000-08-01 00:00:00

abstract：:Six mutants, allelic to ade3, were isolated after mutagenic treatment of a prototrophic strain of yeast. All six grow on medium supplemented with adenine alone and four respond to histidine. Supplementation with adenine plus histidine or methionine inhibits growth, but a mixture of these three is stimulatory. Heteroal...

journal_title：Journal of bacteriology

authors： Lomax CA,Gross TS,Woods RA

更新日期：1971-07-01 00:00:00

abstract：:When incubated in the light under 100% oxygen, wild-type blue-green algae (Anacystis nidulans, Synechococcus cedrorum) die out rapidly at temperatures of 4 to 15 C, and at 35 C (or at 26 C in the case of S. cedrorum) in the absence of CO(2). Photosynthesis is impaired in these cells long before they die. Blocking of p...

journal_title：Journal of bacteriology

authors： Abeliovich A,Shilo M

更新日期：1972-09-01 00:00:00

abstract：:Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...

journal_title：Journal of bacteriology

authors： Chaillou S,Lokman BC,Leer RJ,Posthuma C,Postma PW,Pouwels PH

更新日期：1998-05-01 00:00:00

abstract：:A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosi...

journal_title：Journal of bacteriology

authors： Hattman S,Schlagman S,Cousens L

更新日期：1973-09-01 00:00:00

abstract：:Anabaenopeptins (AP) are bioactive cyclic hexapeptides synthesized nonribosomally in cyanobacteria. APs are characterized by several conserved motifs, including the ureido bond, N-methylation in position 5, and d-Lys in position 2. All other positions of the AP molecule are variable, resulting in numerous structural v...

journal_title：Journal of bacteriology

authors： Christiansen G,Philmus B,Hemscheidt T,Kurmayer R

更新日期：2011-08-01 00:00:00

abstract：:During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...

journal_title：Journal of bacteriology

authors： Severinova E,Severinov K

更新日期：2006-05-01 00:00:00

abstract：:The gene for staphylococcal enterotoxin type E (entE) was cloned from Staphylococcus aureus into plasmid vector pBR322 and introduced into Escherichia coli. A staphylococcal enterotoxin type E-producing E. coli strain was isolated. The complete nucleotide sequence of the cloned structural entE gene and the N-terminal ...

journal_title：Journal of bacteriology

authors： Couch JL,Soltis MT,Betley MJ

更新日期：1988-07-01 00:00:00

abstract：:Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...

journal_title：Journal of bacteriology

authors： Lascelles J,Hatch TP

更新日期：1969-05-01 00:00:00

abstract：:Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...

journal_title：Journal of bacteriology

authors： Yoshimura F,Nikaido H

更新日期：1982-11-01 00:00:00

abstract：:All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...

journal_title：Journal of bacteriology

authors： Ligon LS,Rigel NW,Romanchuk A,Jones CD,Braunstein M

更新日期：2013-10-01 00:00:00

abstract：:The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...

journal_title：Journal of bacteriology

authors： Samaluru H,SaiSree L,Reddy M

更新日期：2007-11-01 00:00:00

abstract：:Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...

journal_title：Journal of bacteriology

authors： Zagotta MT,Wilson DB

更新日期：1990-02-01 00:00:00

abstract：:The participation of tyramine oxidase in the regulation of arylsulfatase synthesis in Salmonella typhimurium was studied. Arylsulfatase synthesis was repressed by inorganic sulfate, cysteine, methionine, or taurine. This repression was relieved by tyramine, octopamine, or dopamine, which induced tyramine oxidase synth...

journal_title：Journal of bacteriology

authors： Murooka Y,Harada T

更新日期：1981-02-01 00:00:00

abstract：:Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,00...

journal_title：Journal of bacteriology

authors： Schulman M,Valentino D

更新日期：1976-10-01 00:00:00

abstract：:A psychrotolerant marine bacterial strain, designated JUB59(T), was isolated from Antarctic surface seawater and classified as a new species of the genus Bizionia. Here, we present the first draft genome sequence for this genus, which suggests interesting features such as UV resistance, hydrolytic exoenzymes, and nitr...

journal_title：Journal of bacteriology

authors： Lanzarotti E,Pellizza L,Bercovich A,Foti M,Coria SH,Vazquez SC,Ruberto L,Hernández EA,Dias RL,Mac Cormack WP,Cicero DO,Smal C,Nicolas MF,Vasconcelos AT,Marti MA,Turjanski AG

更新日期：2011-12-01 00:00:00

abstract：:In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...

journal_title：Journal of bacteriology

authors： Frías JE,Flores E

更新日期：2010-06-01 00:00:00