Abstract:
:Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The cortex peptidoglycan in dacB mutant spores had more peptide side chains, a higher degree of peptide cross-linking, and possibly less muramic acid lactam than that of wild-type spores. These cortex structure parameters were normal in spmA and spmB mutant spores, but these spores did not attain normal spore core dehydration. This defect in spore core dehydration was exaggerated by the additional loss of dacB expression. However, loss of dacB alone did not alter the spore core water content. Spores produced by spmA and spmB mutants germinated faster than did those of the wild type. Spores produced by dacB mutants germinated normally but were delayed in spore outgrowth. Electron microscopy revealed a drastically altered appearance of the cortex in dacB mutants and a minor alteration in an spmA mutant. Measurements of electron micrographs indicate that the ratio of the spore protoplast volume to the sporoplast (protoplast-plus-cortex) volume was increased in dacB and spmA mutants. These results are consistent with spore core water content being the major determinant of spore heat resistance. The idea that loosely cross-linked, flexible cortex peptidoglycan has a mechanical activity involved in achieving spore core dehydration is not consistent with normal core dehydration in spores lacking only dacB.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Popham DL,Illades-Aguiar B,Setlow Pdoi
10.1128/jb.177.16.4721-4729.1995subject
Has Abstractpub_date
1995-08-01 00:00:00pages
4721-9issue
16eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::Polysaccharide-containing material was located by histochemical staining on ultrathin sections of Bacillus cereus and several clostridial species during various phases of growth. These components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. In B. cere...
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更新日期:2000-03-01 00:00:00
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更新日期:1985-12-01 00:00:00
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更新日期:1985-04-01 00:00:00
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更新日期:1992-12-01 00:00:00
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更新日期:2001-12-01 00:00:00
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更新日期:1975-03-01 00:00:00
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更新日期:1983-02-01 00:00:00
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更新日期:1990-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1996-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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更新日期:2018-09-24 00:00:00