Tn5-mediated transposition of plasmid DNA after transduction to Myxococcus xanthus.


:After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by intact Tn5 or IS50 sequences. These results suggest that Tn5-mediated transposition has occurred and provide a method for integrating plasmid DNA into the M. xanthus chromosome without the requirement for homologous recombination.


J Bacteriol


Journal of bacteriology


Downard JS




Has Abstract


1988-10-01 00:00:00












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    authors: Shiio T,McFadden BA

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    pub_type: 杂志文章


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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Webb E,Claas K,Downs DM

    更新日期:1997-07-01 00:00:00