Abstract:
:Integrons constitute a novel family of DNA elements which evolved by site-specific integration of discrete units between two conserved segments. On the In4 integron of Tn1696, a precisely inserted gene cassette of 1,549 bp conferring nonenzymatic chloramphenicol resistance (cmlA) is present between the streptomycin-spectinomycin resistance (aadA2) gene cassette and the 3'-conserved segment of the integron. In this study, we present the nucleotide sequence of the cmlA gene cassette of Tn1696, show its similarity to bacterial efflux systems and other transport proteins, and present evidence for alterations that its expression exerts on bacterial membranes. The cmlA gene cassette apparently carries its own promoter(s), a situation that has not heretofore been observed in the integrons of multiresistance plasmids and transposons of gram-negative bacteria. One or more of these promoters were shown to be functionally active in expressing a cat marker gene from promoter-probe vectors. The putative CmlA polypeptide appears to provoke a reduction of the content of the major porins OmpA and OmpC.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bissonnette L,Champetier S,Buisson JP,Roy PHdoi
10.1128/jb.173.14.4493-4502.1991keywords:
subject
Has Abstractpub_date
1991-07-01 00:00:00pages
4493-502issue
14eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::The symbiotic plasmid of Rhizobium etli CE3 belongs to the RepABC family of plasmid replicons. This family is characterized by the presence of three conserved genes, repA, repB, and repC, encoded by the same DNA strand. A long intergenic sequence (igs) between repB and repC is also conserved in all members of the plas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.11.3117-3124.2000
更新日期:2000-06-01 00:00:00
abstract::An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.801-813.1972
更新日期:1972-09-01 00:00:00
abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4417-4425.1987
更新日期:1987-10-01 00:00:00
abstract::To define additional components of the export machinery of Escherichia coli, I have isolated extragenic suppressors of a mutant [secA(Ts)] that is temperature sensitive for growth and secretion at 37 degrees C. Suppressors that restored growth at 37 degrees C, but that rendered the cell cold sensitive for growth at 28...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.285-291.1985
更新日期:1985-01-01 00:00:00
abstract::Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.12.4077-4085.2005
更新日期:2005-06-01 00:00:00
abstract::Plasmid Rms312, specifying resistance to tetracycline (Tc), chloramphenicol (Cm), streptomycin (Sm), sulfonamide (Su), and mercury chloride (Mer), deletes both Tc and Cm Sm Su Mer determinants at a high frequency in Salmonella typhimurium LT2. S. typhimurium mutants that were stable carriers of Rms312 were isolated by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.145-152.1980
更新日期:1980-04-01 00:00:00
abstract::The ops and tps genes of Myxococcus xanthus have ca. 90% DNA and amino acid sequence homology and are in the same orientation separated by a spacer region of only 1.4 kilobases. The products of the two genes were found to cross-react immunologically, and both were capable of Ca2+-dependent self-assembly on the surface...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.1.121-125.1985
更新日期:1985-07-01 00:00:00
abstract::Zorbamycin (U-30,604E) induces rapid degradation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in Bacillus subtilis cells. DNA degradation is initiated first and is closely followed by the degradation of RNA. No interaction between isolated DNA and zorbamycin is observed. Nucleic acid and protein syntheses...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.30-37.1971
更新日期:1971-10-01 00:00:00
abstract::The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.29-35.1982
更新日期:1982-07-01 00:00:00
abstract::The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.118-127.1977
更新日期:1977-04-01 00:00:00
abstract::By means of the general procedure of Casadaban (J. Mol. Biol. 104: 541-556, 1976), the lac genes carried on a lambda-Mu-1 hybrid phage were inserted into a temperature-inducible Mu-1 prophage that had earlier been inserted into a site near the beginning of the ilvC gene of Escherichia coli strain K-12. Selection of te...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.3.870-875.1977
更新日期:1977-12-01 00:00:00
abstract::5-Azacytidine was found to be bactericidal to Escherichia coli carrying plasmids specifying EcoRII restriction-modification systems, but not to the same strains lacking these plasmids. Of other base analogs tested, only 5(beta-D-ribofuranosyl)isocytidine had similar, although weaker, effects. Plasmids that had lost th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.262-268.1982
更新日期:1982-07-01 00:00:00
abstract::Lockart, Royce Z., Jr. (The University of Texas, Austin). Variations in the abilities of several lines of L cells to produce and to be affected by interferon. J. Bacteriol. 89:117-122. 1965.-The amounts of interferon produced by three different lines of L cells and mouse embryonic cells when inoculated with two strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.1.117-122.1965
更新日期:1965-01-01 00:00:00
abstract::By using the isotope pairs (22)Na-(24)Na and (42)K-(86)Rb, the uptake and retention of Na and K was studied in the salt-tolerant Debaryomyces hansenii and in the less tolerant Saccharomyces cerevisiae at NaCl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. The ratio of K to Na is much higher in the cells than ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.836-845.1969
更新日期:1969-11-01 00:00:00
abstract::Enzymes of tyrosine biosynthesis (prephenate dehydrogenase and arogenate dehydrogenase) were characterized in 90 species currently classified within the genera Pseudomonas, Xanthomonas, and Alcaligenes. Variation in cofactor specificity and regulatory properties of the dehydrogenase proteins allowed the separation of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.247-257.1980
更新日期:1980-10-01 00:00:00
abstract::The sequence of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli K-12 and previous molecular weight determinations indicate that this allosteric enzyme is a monomer of Mr 51,316. The protein is homologous to ATP-dependent phosphoenolpyruvate carboxykinases from Trypanosoma brucei and Sacc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.12.7151-7156.1990
更新日期:1990-12-01 00:00:00
abstract::In Escherichia coli, nine essential cell division proteins are known to localize to the division septum. FtsL is a 13-kDa bitopic membrane protein with a short cytoplasmic N-terminal domain, a membrane-spanning segment, and a periplasmic domain that has a repeated heptad motif characteristic of leucine zippers. Here, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.116-129.2000
更新日期:2000-01-01 00:00:00
abstract::Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for huma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.141-148.1967
更新日期:1967-07-01 00:00:00
abstract::The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.657-663.1983
更新日期:1983-08-01 00:00:00
abstract::Thiamine pyrophosphate (TPP) is synthesized de novo in Salmonella typhimurium and is a required cofactor for many enzymes in the cell. Five kinase activities have been implicated in TPP synthesis, which involves joining a 4-methyl-5-(beta-hydroxyethyl)thiazole (THZ) moiety and a 4-amino-5-hydroxymethyl-2-methylpyrimid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4894-4900.1997
更新日期:1997-08-01 00:00:00
abstract::Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.5.2385-2387.1988
更新日期:1988-05-01 00:00:00
abstract::We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3750-3751.1988
更新日期:1988-08-01 00:00:00
abstract::Thermococcus sp. strain CL1 is a hyperthermophilic, anaerobic, and heterotrophic archaeon isolated from a Paralvinella sp. polychaete worm living on an active deep-sea hydrothermal sulfide chimney on the Cleft Segment of the Juan de Fuca Ridge. To further understand the distinct characteristics of this archaeon at the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01016-12
更新日期:2012-09-01 00:00:00
abstract::We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.621-628.2002
更新日期:2002-02-01 00:00:00
abstract::The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-13
更新日期:2013-08-01 00:00:00
abstract::A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.500-508.1981
更新日期:1981-08-01 00:00:00
abstract::Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.1.206-211.1995
更新日期:1995-01-01 00:00:00
abstract::The c subunit of Streptococcus mutans ATP synthase (FoF1) is functionally exchangeable with that of Escherichia coli, since E. coli with a hybrid FoF1 is able to grow on minimum succinate medium through oxidative phosphorylation. E. coli F1 bound to the hybrid Fo with the S. mutans c subunit showed N,N'-dicyclohexylca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00542-13
更新日期:2013-11-01 00:00:00
abstract::In a horse serum-based medium containing a full complement of fatty acids, cells of Spiroplasma citri were seen to preferentially incorporate palmitic acid. In the same medium, which had a steryl ester-to-sterol ratio of 3.64, a steryl ester-to-sterol ratio of 0.23 was seen in the cells, cholesterol being preferential...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.946-954.1976
更新日期:1976-03-01 00:00:00
abstract:UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01010-15
更新日期:2016-04-28 00:00:00