Abstract:
:A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology under these conditions correlated well with maximal expression of glycolytic enzymes and with expression of some polypeptides characteristic of anaerobic growth. Aerobic growth of the mutant on amino acids as the sole carbon source resulted in growth in the mycelial morphology. The mutant was fully capable of oxidative metabolism as judged by its ability to grow on amino acids, respiratory capacity, and complement of tricarboxylic acid cycle enzymes. The results support the hypothesis that oxygen controls both the expression of glycolytic enzymes and the expression of proteins involved in morphogenesis. Moreover, they suggest that there are common regulatory elements in the control of these two classes of gene products. Abnormally high levels of aconitase and isocitrate dehydrogenase in the mutant are consistent with the proposal that pool sizes of citrate may act as a regulator of genes responsive to environmental oxygen concentration.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Borgia PT,Gokul NK,Phillips GJdoi
10.1128/JB.164.3.1049-1056.1985subject
Has Abstractpub_date
1985-12-01 00:00:00pages
1049-56issue
3eissn
0021-9193issn
1098-5530journal_volume
164pub_type
杂志文章abstract::The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase. Our previous results showed that IraD determines RpoS steady-state levels during exponential growth and mediates its st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00412-09
更新日期:2009-12-01 00:00:00
abstract::Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to par...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.5.1705-1721.1967
更新日期:1967-05-01 00:00:00
abstract::The marRAB operon is one of two operons in the mar locus of Escherichia coli that are divergently transcribed from a central regulatory region, marO. The marRAB operon, transcribed from marOII, controls intrinsic resistance or susceptibility to multiple antibiotics and is inducible by structurally unrelated compounds ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.12.3414-3419.1995
更新日期:1995-06-01 00:00:00
abstract::Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.723-728.1982
更新日期:1982-08-01 00:00:00
abstract::The conditions for the infection of Bacillus stearothermophilus 4S with TP-1C phage deoxyribonucleic acid (DNA) are described. Cells from log-phase cultures are the most susceptible to phage DNA infection (transfection). A cellular component (competence factor) which enhances transfection is released into the culture ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.960-965.1971
更新日期:1971-06-01 00:00:00
abstract::We previously reported that group D streptococci exhibited immunoglobulin G (IgG)-binding activity and that a 52-kDa IgG-binding protein was present in all Streptococcus suis strains examined (B. Serhir, R. Higgins, B. Foiry, and M. Jacques, J. Gen. Microbiol. 139:2953-2958, 1993). The objective of the present study w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.13.3830-3836.1995
更新日期:1995-07-01 00:00:00
abstract::Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.4.815-819.1966
更新日期:1966-10-01 00:00:00
abstract::In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.375-382.1972
更新日期:1972-08-01 00:00:00
abstract::Toxin-antitoxin (TA) loci consist of two genes in an operon, encoding a stable toxin and an unstable antitoxin. The expression of toxin leads to cell growth arrest and sometimes bacterial death, while the antitoxin prevents the cytotoxic activity of the toxin. In this study, we show that the chromosome of Yersinia pes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00336-10
更新日期:2010-07-01 00:00:00
abstract::The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1153-1161.1982
更新日期:1982-09-01 00:00:00
abstract::Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4721-4729.1995
更新日期:1995-08-01 00:00:00
abstract::In order to study the Salmonella typhimurium cobalamin biosynthetic pathway, the S. typhimurium cob operon was isolated and cloned into Escherichia coli. This approach has given the new host of the cob operon the ability to make cobalamins de novo, an ability that had probably been lost by this organism. In total, 20 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.753-767.1996
更新日期:1996-02-01 00:00:00
abstract::Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsibl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00892-06
更新日期:2006-11-01 00:00:00
abstract::Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PE...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00508-08
更新日期:2008-09-01 00:00:00
abstract::The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05990-11
更新日期:2012-05-01 00:00:00
abstract::The symbiotic plasmid of Rhizobium etli CE3 belongs to the RepABC family of plasmid replicons. This family is characterized by the presence of three conserved genes, repA, repB, and repC, encoded by the same DNA strand. A long intergenic sequence (igs) between repB and repC is also conserved in all members of the plas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.11.3117-3124.2000
更新日期:2000-06-01 00:00:00
abstract::The distribution of the extracellular enzyme, thiaminase I, was determined for logarithmically growing cultures of Bacillus thiaminolyticus. About 60% of the enzyme is associated with the cells throughout the growth cycle. The remainder of the enzyme is in the culture medium. The release of the cell-bound thiaminase I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.949-956.1973
更新日期:1973-09-01 00:00:00
abstract::The Methanococcus maripaludis energy-conserving hydrogenase B (Ehb) generates low potential electrons required for autotrophic CO(2) assimilation. To analyze the importance of individual subunits in Ehb structure and function, markerless in-frame deletions were constructed in a number of M. maripaludis ehb genes. Thes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01446-09
更新日期:2010-08-01 00:00:00
abstract::In the bacterium Escherichia coli, oxidized pyrimidines are removed by two DNA glycosylases, endonuclease III and endonuclease VIII (endo VIII), encoded by the nth and nei genes, respectively. Double mutants lacking both of these activities exhibit a high spontaneous mutation frequency, and here we show that all of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6396-6402.1999
更新日期:1999-10-01 00:00:00
abstract::Bacteriophage phi29 protein p6 is a viral architectural protein, which binds along the whole linear phi29 DNA in vivo and is involved in initiation of DNA replication and transcription control. Protein p1 is a membrane-associated viral protein, proposed to attach the viral genome to the cell membrane. Protein p17 is i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8401-8406.2004
更新日期:2004-12-01 00:00:00
abstract::Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. II. Transduction of transmissible drug-resistance (R) factors with phage epsilon. J. Bacteriol. 86:1332-1338. 1963.-Transmissible drug-resistance (R) factors, which transfer resi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.6.1332-1338.1963
更新日期:1963-12-01 00:00:00
abstract::Anaerobic growth of Klebsiella aerogenes NCDO 711 (NCTC 418) on citrate was dependent on the presence of Na+ in the medium, and fermentation of citrate was mediated via the fermentation pathway enzymes, citrate lyase and a Na+-dependent oxalacetate decarboxylase. This confirms the previous findings on strain NCTC 418....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.2.468-473.1975
更新日期:1975-05-01 00:00:00
abstract::The intestinal pathogen Clostridium difficile is known to grow only within the intestines of mammals, yet little is known about how the bacterium subsists in this environment. In the intestine, C. difficile must contend with innate defenses within the host, such as cationic antimicrobial peptides (CAMPs) produced by t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00166-13
更新日期:2013-06-01 00:00:00
abstract::Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00728-13
更新日期:2013-09-01 00:00:00
abstract::Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in most bacterial species are hypersensitive to UV damage, surprisingly a B. abortus recA null mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-05
更新日期:2006-07-01 00:00:00
abstract::A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4655-4666.1989
更新日期:1989-09-01 00:00:00
abstract::Goldman, Manuel (The University of Michigan, Ann Arbor), and Harold J. Blumenthal. Pathways of glucose catabolism in Bacillus cereus. J. Bacteriol. 87:377-386. 1964.-Estimates by a radiorespirometric method of the pathways of glucose catabolism of resting-cell suspensions of Bacillus cereus strain terminalis indicate ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.377-386.1964
更新日期:1964-02-01 00:00:00
abstract::The photosynthetic bacterium Rhodobacter capsulatus contains two [NiFe]hydrogenases: an energy-generating hydrogenase, HupSL, and a regulatory hydrogenase, HupUV. The synthesis of HupSL is specifically activated by H(2) through a signal transduction cascade comprising three proteins: the H(2)-sensing HupUV protein, th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.24.7111-7119.2003
更新日期:2003-12-01 00:00:00
abstract::We previously showed that dprA is required for efficient processing of linear DNA during cellular transformation in Haemophilus influenzae. In this study the transcriptional regulation of dprA and two downstream genes, dprB and dprC, is examined. We demonstrate by Northern blot analysis that the dprABC genes are trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4815-4820.1997
更新日期:1997-08-01 00:00:00
abstract::The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.86-93.1991
更新日期:1991-01-01 00:00:00