High-molecular-weight complexes of RsbR and paralogues in the environmental signaling pathway of Bacillus subtilis.

Abstract:

:Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsible for the transcription of the general stress response genes. Some of the regulators of sigma(B), such as RsbR and RsbS, are known to associate in a 25S complex, called the stressosome, that can bind RsbT until RsbT phosphorylates target residues in RsbR and RsbS. To date, the RsbR-RsbS complex appears to be the most upstream component of the sigma(B) regulatory pathway. This large structure is thought to play an important role in sensing and/or integrating signals from different physical stresses. The roles of the paralogues of RsbR that are found in B. subtilis remain unclear. We describe here how the RsbR paralogues copurify with RsbR from B. subtilis cell lysates, and we demonstrate in vitro that the paralogues form large complexes either with RsbS or with a prepurified RsbR-RsbS binary complex. We conclude from these biochemical studies that stressosomes in B. subtilis cells contain minimally RsbS and all of the RsbT-phosphorylatable RsbR paralogues.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Delumeau O,Chen CC,Murray JW,Yudkin MD,Lewis RJ

doi

10.1128/JB.00892-06

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

7885-92

issue

22

eissn

0021-9193

issn

1098-5530

pii

JB.00892-06

journal_volume

188

pub_type

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