A- and T-tract-mediated intrinsic curvature in native DNA between the binding site of the upstream activator NtrC and the nifLA promoter of Klebsiella pneumoniae facilitates transcription.

abstract：:Incubation of ultraviolet-irradiated Escherichia coli B/r cultures with 0.7% Triton X-100 resulted in a large decrease in turbidity. Under phase-contrast optics, most of the irradiated detergent-treated cells were smaller than normal and of low phase density; only a small percentage were normal or larger than normal a...

journal_title：Journal of bacteriology

authors： Schenley RL,Fisher WD,Swenson PA,Khachatourians GG

更新日期：1976-05-01 00:00:00

abstract：:The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were...

journal_title：Journal of bacteriology

authors： Kasian PA,Pittard J

更新日期：1984-10-01 00:00:00

abstract：:The DNA region encoding early nodulation functions of Bradyrhizobium japonicum 3I1b110 (I110) was isolated by its homology to the functionally similar region from Rhizobium meliloti. Isolation of a number of overlapping recombinant clones from this region allowed the construction of a restriction map of the region. Th...

journal_title：Journal of bacteriology

authors： Russell P,Schell MG,Nelson KK,Halverson LJ,Sirotkin KM,Stacey G

更新日期：1985-12-01 00:00:00

abstract：UNLABELLED:Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for thymineless death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the...

journal_title：Journal of bacteriology

authors： Itsko M,Schaaper RM

更新日期：2016-05-13 00:00:00

abstract：:The enhancer-binding protein NIFA is required for transcriptional activation of nif promoters by the alternative holoenzyme form of RNA polymerase, which contains the sigma factor sigma 54 (sigma N). NIFA hydrolyzes nucleoside triphosphates to catalyze the isomerization of closed promoter complexes to transcriptionall...

journal_title：Journal of bacteriology

authors： Eydmann T,Söderbäck E,Jones T,Hill S,Austin S,Dixon R

更新日期：1995-03-01 00:00:00

abstract：:The effect of sublethal, midlethal, and lethal doses of total-body X irradiation on the fecal microbial population of the rat was studied. No changes were observed in animals receiving sublethal doses of X ray. Midlethal and lethal doses produced an increase in the numbers of fecal coliforms, staphylococci, streptococ...

journal_title：Journal of bacteriology

authors： Klainer AS,Gorbach S,Weinstein L

更新日期：1967-08-01 00:00:00

abstract：:plsB mutants of Escherichia coli are sn-glycerol 3-phosphate auxotrophs which owe their requirement to a K(m) defect in sn-glycerol 3-phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. We have located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene de...

journal_title：Journal of bacteriology

authors： Cronan JE Jr,Bell RM

更新日期：1974-10-01 00:00:00

abstract：:Melanin production is a major virulence factor for Cryptococcus neoformans, an organism causing life-threatening infections in an estimated 10% of AIDS patients. In order to characterize the events involved in melanin synthesis, an enzyme having diphenol oxidase activity was purified and its gene was cloned. The enzym...

journal_title：Journal of bacteriology

authors： Williamson PR

更新日期：1994-02-01 00:00:00

abstract：:LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...

journal_title：Journal of bacteriology

authors： Bowers CW,Lau F,Silhavy TJ

更新日期：2003-10-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. Growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. Mutants, created by transposon mutage...

journal_title：Journal of bacteriology

authors： McCarter LL,Wright ME

更新日期：1993-06-01 00:00:00

abstract：:Fitz-James, Philip (University of Western Ontario, London, Canada). Electron microscopy of Bacillus megaterium undergoing isolation of its nucelar bodies. J. Bacteriol. 87:1202-1210. 1964.-The various steps of treatment leading to the isolation of nuclear bodies were followed by thin-section electron microscopy. Nucle...

journal_title：Journal of bacteriology

authors： Fitz-James P

更新日期：1964-05-01 00:00:00

abstract：:A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...

journal_title：Journal of bacteriology

authors： Rumbak E,Rawlings DE,Lindsey GG,Woods DR

更新日期：1991-11-01 00:00:00

abstract：:The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...

journal_title：Journal of bacteriology

authors： Osman SF,Fett WF

更新日期：1989-03-01 00:00:00

abstract：:Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...

journal_title：Journal of bacteriology

authors： FRAMPTON EW,BRINKLEY BR

更新日期：1965-08-01 00:00:00

abstract：:The presence of the altered lysine transfer ribonucleic acid (tRNA) in Bacillus subtilis spores is strongly dependent on the medium on which the cells were sporulated. Cells sporulated on synthetic media or dilute complex media contain little or none of the new component, whereas those sporulated on concentrated compl...

journal_title：Journal of bacteriology

authors： Lazzarini RA,Santangelo E

更新日期：1967-07-01 00:00:00

abstract：:Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

journal_title：Journal of bacteriology

authors： Tisa LS,Ensign JC

更新日期：1988-07-01 00:00:00

abstract：:t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). Th...

journal_title：Journal of bacteriology

authors： Moussa SH,Lawler JL,Young R

更新日期：2014-06-01 00:00:00

abstract：:The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...

journal_title：Journal of bacteriology

authors： Hackstadt T,Todd WJ,Caldwell HD

更新日期：1985-01-01 00:00:00

abstract：:The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli. The envZ11 mutation results in the OmpF- OmpC-constitutive phenotype. A mutant which suppressed defects caused by the envZ11 mu...

journal_title：Journal of bacteriology

authors： Matsuyama S,Mizuno T,Mizushima S

更新日期：1986-12-01 00:00:00

abstract：:A polysaccharide-rich particulate fraction was isolated from cytoplasmic extracts of Candida albicans by a procedure using differential centrifugation. The polysaccharide particles obtained after purification with deoxycholate treatment were essentially free of nitrogen and were identified chemically as polyglucosan, ...

journal_title：Journal of bacteriology

authors： Yamaguchi H,Kanda Y,Iwata K

更新日期：1974-10-01 00:00:00

abstract：:The general aromatic amino acid permease, AroP, of Escherichia coli is responsible for the active transport of phenylalanine, tyrosine, and tryptophan. A proposed topological model for the AroP permease, consisting of 12 hydrophobic transmembrane spans connected by hydrophilic loops, is very similar to that of the clo...

journal_title：Journal of bacteriology

authors： Cosgriff AJ,Pittard AJ

更新日期：1997-05-01 00:00:00

abstract：:The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF- mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the respon...

journal_title：Journal of bacteriology

authors： Zeilstra-Ryalls JH,Schornberg KL

更新日期：2006-01-01 00:00:00

abstract：:Rosenkranz, Herbert S. (Columbia University, New York, N.Y.), Howard S. Carr, and Harry M. Rose. Phenethyl alcohol. II. Effect on thymine-requiring Escherichia coli. J. Bacteriol. 89:1370-1373. 1965.-Phenethyl alcohol (PEA) at levels (0.05%) presumed to interfere with messenger ribonucleic acid synthesis also inhibits...

journal_title：Journal of bacteriology

authors： ROSENKRANZ HS,CARR HS,ROSE HM

更新日期：1965-05-01 00:00:00

abstract：:Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

journal_title：Journal of bacteriology

authors： Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

更新日期：1997-11-01 00:00:00

abstract：:The Gram-positive bacterium Streptococcus pyogenes (also called group A Streptococcus [GAS]), is found strictly in humans and is capable of causing a wide variety of infections. Here we demonstrate that serine catabolism in GAS is controlled by the transcriptional regulator Spy49_0126c. We have designated this regulat...

journal_title：Journal of bacteriology

authors： LaSarre B,Federle MJ

更新日期：2011-04-01 00:00:00

abstract：:Dowell, C. E. (The University of Texas, Dallas) and E. D. Rosenblum. Staphylococcal transducing particle. J. Bacteriol. 84:1076-1079. 1962.-When novobiocin-resistant transductants were isolated under conditions that permitted superinfection, almost all the clones were lysogenic for the transducing phage. If superinfec...

journal_title：Journal of bacteriology

authors： Dowell CE,Rosenblum ED

更新日期：1962-11-01 00:00:00

abstract：:Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsI, ftsQ, or ftsZ) required for septation were constructed. Such mutants were able to grow for about two mass doublings at a normal rate at the restri...

journal_title：Journal of bacteriology

authors： Begg KJ,Donachie WD

更新日期：1985-08-01 00:00:00

abstract：:Sphingomonas wittichii RW1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. We used inverse PCR to amplify dxnB2, a gene encoding one of three meta-cleavage product (MCP) hydrolases identified in the organism that are homologues of BphD involved in biphenyl catabolism. Purified DxnB2 catalyz...

journal_title：Journal of bacteriology

authors： Seah SY,Ke J,Denis G,Horsman GP,Fortin PD,Whiting CJ,Eltis LD

更新日期：2007-06-01 00:00:00

abstract：:The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alp...

journal_title：Journal of bacteriology

authors： Yamaguchi K,Nagata Y,Maruo B

更新日期：1974-08-01 00:00:00