Interaction between two regulatory proteins in osmoregulatory expression of ompF and ompC genes in Escherichia coli: a novel ompR mutation suppresses pleiotropic defects caused by an envZ mutation.

abstract：:Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...

journal_title：Journal of bacteriology

authors： Shaw JH,Clewell DB

更新日期：1985-11-01 00:00:00

abstract：:The heat shock response of the groESL operon of Agrobacterium tumefaciens was studied at the RNA level. The operon was found to be activated under heat shock conditions and transcribed as a polycistronic mRNA that contains the groES and groEL genes. After activation, the polycistronic mRNA appeared to be cleaved betwe...

journal_title：Journal of bacteriology

authors： Segal G,Ron EZ

更新日期：1995-02-01 00:00:00

abstract：:Although nitrate stimulated the capacity of Clostridium thermoautotrophicum and Clostridium thermoaceticum to oxidize (utilize) substrates under heterotrophic conditions, it inhibited autotrophic H2-CO2-dependent growth. Under basal medium conditions, nitrate was also inhibitory to the use of one-carbon substrates (i....

journal_title：Journal of bacteriology

authors： Fröstl JM,Seifritz C,Drake HL

更新日期：1996-08-01 00:00:00

abstract：:Flagellar motility is an important determinant of Campylobacter jejuni that is required for promoting interactions with various hosts to promote gastroenteritis in humans or commensal colonization of many animals. In a previous study, we identified a nonmotile mutant of C. jejuni 81-176 with a transposon insertion in ...

journal_title：Journal of bacteriology

authors： Sommerlad SM,Hendrixson DR

更新日期：2007-01-01 00:00:00

abstract：:A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...

journal_title：Journal of bacteriology

authors： Rumbak E,Rawlings DE,Lindsey GG,Woods DR

更新日期：1991-11-01 00:00:00

abstract：:The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...

journal_title：Journal of bacteriology

authors： Köplin R,Arnold W,Hötte B,Simon R,Wang G,Pühler A

更新日期：1992-01-01 00:00:00

abstract：:Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...

journal_title：Journal of bacteriology

authors： Benning C,Somerville CR

更新日期：1992-10-01 00:00:00

abstract：:Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...

journal_title：Journal of bacteriology

authors： Alonso-Vega P,Normand P,Bacigalupe R,Pujic P,Lajus A,Vallenet D,Carro L,Coll P,Trujillo ME

更新日期：2012-08-01 00:00:00

abstract：:Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

journal_title：Journal of bacteriology

authors： Chaffin DO,Beres SB,Yim HH,Rubens CE

更新日期：2000-08-01 00:00:00

abstract：:The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In t...

journal_title：Journal of bacteriology

authors： Lee HS,Cho Y,Lee JH,Kang SG

更新日期：2008-04-01 00:00:00

abstract：:A nitrate-utilizing strain of marine bacteria was isolated in which luciferase was inducible by l-arginine. The induction was highly specific; structural analogues of arginine were ineffective, as were other natural amino acids. Several metabolites of arginine acted as weak inducers but did not affect the rate of indu...

journal_title：Journal of bacteriology

authors： Coffey JJ

更新日期：1967-11-01 00:00:00

abstract：:Drugs known to interrupt the chain of protein synthesis (streptomycin, chloramphenicol, ethidium bromide) appeared to affect production of the group D lysin by Streptococcus zymogenes by inhibiting growth. Drugs which block cell wall synthesis (vancomycin, bacitracin, d-cycloserine, and phosphonomycin) inhibited lytic...

journal_title：Journal of bacteriology

authors： Werth JM,Jackson RW

更新日期：1971-11-01 00:00:00

abstract：:The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...

journal_title：Journal of bacteriology

authors： Padhy RN,Hottat FG,Coene MM,Hoet PP

更新日期：1988-04-01 00:00:00

abstract：:Bacillus subtilis OhrR is the prototype for the one-Cys family of organic peroxide-sensing regulatory proteins. Mutational analyses indicate that the high sensitivity of the active site cysteine (C15) to peroxidation requires three Tyr residues. Y29 and Y40 from the opposing subunit of the functional dimer hydrogen bo...

journal_title：Journal of bacteriology

authors： Soonsanga S,Fuangthong M,Helmann JD

更新日期：2007-10-01 00:00:00

abstract：:The Put3p and Gal4p transcriptional activators are members of a distinct class of fungal regulators called the Cys(6) Zn(II)(2) binuclear cluster family. This family includes over 50 different Saccharomyces cerevisiae proteins that share a similar domain organization. Gal4p activates the genes of the galactose utiliza...

journal_title：Journal of bacteriology

authors： D'Alessio M,Brandriss MC

更新日期：2000-07-01 00:00:00

abstract：:A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire...

journal_title：Journal of bacteriology

authors： Bible AN,Stephens BB,Ortega DR,Xie Z,Alexandre G

更新日期：2008-10-01 00:00:00

abstract：:Bacillus subtilis strains carrying div-341 or sacU mutations, or both, have been characterized to reveal the roles of both genes in the initiation of sporulation, as well as in cell division and exoenzyme secretion. Both mutations were closely linked by transformation and caused the pleiotropic effects on sporulation ...

journal_title：Journal of bacteriology

authors： Sadaie Y,Kada T

更新日期：1985-08-01 00:00:00

abstract：:We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

journal_title：Journal of bacteriology

authors： Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

更新日期：1985-01-01 00:00:00

abstract：:The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

journal_title：Journal of bacteriology

authors： Hille J,van Kan J,Klasen I,Schilperoort R

更新日期：1983-05-01 00:00:00

abstract：:Peripheral hyphae were separated from the remaining thallus of Rhizoctonia solani in exponential and stationary phases of growth. The QO(2) in whole cells of peripheral hyphae from young fungal colonies was on the average 2.6 times and the protein content 1.6 times greater than in peripheral hyphae from old fungal col...

journal_title：Journal of bacteriology

authors： Skowronski BS,Gottlieb D

更新日期：1970-11-01 00:00:00

abstract：:The magnetosomes of many magnetotactic bacteria consist of membrane-enveloped magnetite crystals, whose synthesis is favored by a low redox potential. However, the cellular redox processes governing the biomineralization of the mixed-valence iron oxide have remained unknown. Here, we show that in the alphaproteobacter...

journal_title：Journal of bacteriology

authors： Li Y,Katzmann E,Borg S,Schüler D

更新日期：2012-09-01 00:00:00

abstract：:The elemental composition of individual cells of rapidly frozen and cryosectioned Escherichia coli B was measured with electron optical microanalytic methods. The Ca content was high (26.2 mmol/kg) in a 10-nm-wide region of the cell envelope. Amounts of cytoplasmic Ca in actively dividing cells were significantly high...

journal_title：Journal of bacteriology

authors： Chang CF,Shuman H,Somlyo AP

更新日期：1986-09-01 00:00:00

abstract：:An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K. ...

journal_title：Journal of bacteriology

authors： Matney TS,MacDougall NL,Butler MA,Suit JC

更新日期：1970-10-01 00:00:00

abstract：:Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

journal_title：Journal of bacteriology

authors： van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

更新日期：1999-03-01 00:00:00

abstract：:When cells of Streptococcus faecalis ATCC 9790 were incubated at temperatures above 10 C before being frozen for freeze-fracture, a random distribution of particles was observed on the outer fracture face of the freeze-cleaved cell membrane. However, when cells were incubated below 10 C before freezing, particleless p...

journal_title：Journal of bacteriology

authors： Tsien HC,Higgins ML

更新日期：1974-05-01 00:00:00

abstract：:The marRAB operon is one of two operons in the mar locus of Escherichia coli that are divergently transcribed from a central regulatory region, marO. The marRAB operon, transcribed from marOII, controls intrinsic resistance or susceptibility to multiple antibiotics and is inducible by structurally unrelated compounds ...

journal_title：Journal of bacteriology

authors： Seoane AS,Levy SB

更新日期：1995-06-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli O157:H7 (EHEC) causes bloody diarrhea and hemolytic-uremic syndrome. EHEC encodes the sRNA chaperone Hfq, which is important in posttranscriptional regulation. In EHEC strain EDL933, Hfq acts as a negative regulator of the locus of enterocyte effacement (LEE), which encodes most of t...

journal_title：Journal of bacteriology

authors： Kendall MM,Gruber CC,Rasko DA,Hughes DT,Sperandio V

更新日期：2011-12-01 00:00:00

abstract：:Eight different derivatives of R plasmid RP1 with thermosensitive mutations affecting maintenance in Escherichia coli and Pseudomonas aeruginosa were introduce into Rhizobium meliloti. None of the plasmids showed a thermosensitive character in R. meliloti. On the other hand, a certain deletion mutation in RP1 was foun...

journal_title：Journal of bacteriology

authors： Hooykaas PJ,den Dulk-Ras H,Schilperoort RA

更新日期：1982-04-01 00:00:00

abstract：:The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

journal_title：Journal of bacteriology

authors： Anderson DG,Moseley SL

更新日期：1988-10-01 00:00:00

abstract：:Expression of the major porin structural genes, ompF and ompC, is influenced by medium osmotic strength and requires the products of two regulatory genes, ompR and envZ. To help define the sites required for the expression of both porin genes, we have used a novel selection to identify mutations that decrease ompF tra...

journal_title：Journal of bacteriology

authors： Taylor RK,Garrett S,Sodergren E,Silhavy TJ

更新日期：1985-06-01 00:00:00