Site-directed mutagenesis in Escherichia coli of a stable R772::Ti cointegrate plasmid from Agrobacterium tumefaciens.

abstract：:In Rhizobium meliloti, an aspartate aminotransferase (AspAT) encoded within a 7.3-kb HindIII fragment was previously shown to be required for symbiotic nitrogen fixation and aspartate catabolism (V. K. Rastogi and R.J. Watson, J. Bacteriol. 173:2879-2887, 1991). A gene coding for an aromatic aminotransferase located w...

journal_title：Journal of bacteriology

authors： Watson RJ,Rastogi VK

更新日期：1993-04-01 00:00:00

abstract：:The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...

journal_title：Journal of bacteriology

authors： Chatfield LK,Orr E,Boulnois GJ,Wilkins BM

更新日期：1982-12-01 00:00:00

abstract：:Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...

journal_title：Journal of bacteriology

authors： Higashi T,Iwasaki Y,Ohnishi Y,Horinouchi S

更新日期：2007-05-01 00:00:00

abstract：:A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication...

journal_title：Journal of bacteriology

authors： Witkin EM,Roegner-Maniscalco V

更新日期：1992-06-01 00:00:00

abstract：:Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for huma...

journal_title：Journal of bacteriology

authors： Cohen IR

更新日期：1967-07-01 00:00:00

abstract：:FNR is an Escherichia coli transcription factor that regulates the transcription of many genes in response to anaerobiosis. We have constructed a series of artificial FNR-dependent promoters, based on the melR promoter, in which a consensus FNR binding site was centered at position -41.5 relative to the transcription ...

journal_title：Journal of bacteriology

authors： Barnard AM,Green J,Busby SJ

更新日期：2003-10-01 00:00:00

abstract：:The hutC gene in Pseudomonas putida encodes a repressor protein that negatively regulates the expression of all hut genes. We have overexpressed this cloned hutC gene in Escherichia coli to identify P. putida hut regions that could specifically bind the repressor. Ten restriction fragments, some of which were partiall...

journal_title：Journal of bacteriology

authors： Hu L,Allison SL,Phillips AT

更新日期：1989-08-01 00:00:00

abstract：:The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

journal_title：Journal of bacteriology

authors： Barnett TC,Scott JR

更新日期：2002-04-01 00:00:00

abstract：:Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

journal_title：Journal of bacteriology

authors： Caparrós M,Pisabarro AG,de Pedro MA

更新日期：1992-09-01 00:00:00

abstract：:The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in ana...

journal_title：Journal of bacteriology

authors： Baruah A,Lindsey B,Zhu Y,Nakano MM

更新日期：2004-03-01 00:00:00

abstract：:A new insertion element, IS1549, was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isola...

journal_title：Journal of bacteriology

authors： Plikaytis BB,Crawford JT,Shinnick TM

更新日期：1998-03-01 00:00:00

abstract：:The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

journal_title：Journal of bacteriology

authors： Sundaram TK,Fincham JR

更新日期：1968-03-01 00:00:00

abstract：:Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as...

journal_title：Journal of bacteriology

authors： Watson N,Dunyak DS,Rosey EL,Slonczewski JL,Olson ER

更新日期：1992-01-01 00:00:00

abstract：:We isolated pili from Escherichia coli SS142. These pili had a diameter of 6 nm and an average length of 400 nm. They were composed of subunits with a molecular weight of 18,000. Their amino acid composition was determined; methionine and proline were not detected. The isolated pili retained mannose-resistant hemagglu...

journal_title：Journal of bacteriology

authors： Mett H,Kloetzlen L,Vosbeck K

更新日期：1983-02-01 00:00:00

abstract：:Rhodobacter capsulatus mutants unable to fix nitrogen were isolated by random transposon Tn5 mutagenesis. The Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomal regions designated A, B, and C. The majority of Tn5 insertions (21 mutants) map within nif region A, characterized by two Cl...

journal_title：Journal of bacteriology

authors： Klipp W,Masepohl B,Pühler A

更新日期：1988-02-01 00:00:00

abstract：:Synthesis of wild-type Neurospora crassa assimilatory nitrate reductase is induced in the presence of nitrate ions and repressed in the presence of ammonium ions. Effects of several Neurospora mutations on the regulation of this enzyme are shown: (i) the mutants, nit-1 and nit-3, involving separate lesions, lack reduc...

journal_title：Journal of bacteriology

authors： Dantzig AH,Zurowski WK,Ball TM,Nason A

更新日期：1978-02-01 00:00:00

abstract：:Chemical, physical, and immunological properties of the envelope antigen of Yersinia pestis strains have been investigated. The antigen consists of two components with isoelectric points (pI) of 4.6 and 4.8. One component (pI 4.6) is a protein bound to a small carbohydrate moiety identified as an oligomeric galactan; ...

journal_title：Journal of bacteriology

authors： Bennett LG,Tornabene TG

更新日期：1974-01-01 00:00:00

abstract：:The colicinogenic B factor, transferred from Escherichia coli strain K77 (and termed ColB2-K77 or ColB2) to an E. coli K12 F(-) strain, is capable of promoting its own transfer to other K12 F(-) strains at a low rate (from LFC cultures) which can be increased under special conditions (HFC cultures). LFC cultures of K1...

journal_title：Journal of bacteriology

authors： Hausmann C,Clowes RC

更新日期：1971-09-01 00:00:00

abstract：:Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...

journal_title：Journal of bacteriology

authors： Perez E,Zheng H,Stock AM

更新日期：2006-06-01 00:00:00

abstract：:With the exception of Escherichia coli lysyl-tRNA synthetase, the genes coding for the different aminoacyl-tRNA synthetases in procaryotes are always unique. Here we report on the occurrence and cloning of two genes (thrSv and thrS2), both encoding functional threonyl-tRNA synthetase in Bacillus subtilis. The two prot...

journal_title：Journal of bacteriology

authors： Putzer H,Brakhage AA,Grunberg-Manago M

更新日期：1990-08-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...

journal_title：Journal of bacteriology

authors： DANIEL JW,BABCOCK KL,SIEVERT AH,RUSCH HP

更新日期：1963-08-01 00:00:00

abstract：:Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...

journal_title：Journal of bacteriology

authors： Gregory ST,Cate JH,Dahlberg AE

更新日期：2001-07-01 00:00:00

abstract：:The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...

journal_title：Journal of bacteriology

authors： Beck C,von Meyenburg HK

更新日期：1968-08-01 00:00:00

abstract：:A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...

journal_title：Journal of bacteriology

authors： Bankaitis VA,Kline EL

更新日期：1981-08-01 00:00:00

abstract：:The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...

journal_title：Journal of bacteriology

authors： Kingsbury DT

更新日期：1967-09-01 00:00:00

abstract：:We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...

journal_title：Journal of bacteriology

authors： Ralt D,Wishnok JS,Fitts R,Tannenbaum SR

更新日期：1988-01-01 00:00:00

abstract：:Replication initiator proteins in bacteria not only allow DNA replication but also often regulate the rate of replication initiation as well. The regulation is mediated by limiting the synthesis or availability of initiator proteins. The applicability of this principle is demonstrated here for RctB, the replication in...

journal_title：Journal of bacteriology

authors： Pal D,Venkova-Canova T,Srivastava P,Chattoraj DK

更新日期：2005-11-01 00:00:00

abstract：:Hauge, Jens G. (National Institute for Public Health, Oslo, Norway). Glucose dehydrogenation in bacteria: a comparative study. J. Bacteriol. 82:609-614. 1961.-Extracts of a series of bacteria reported to convert glucose to gluconic acid via particulate enzyme systems were fractionated by differential centrifugation in...

journal_title：Journal of bacteriology

authors： HAUGE JG

更新日期：1961-10-01 00:00:00

abstract：:Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...

journal_title：Journal of bacteriology

authors： LeVier K,Walker GC

更新日期：2001-11-01 00:00:00

abstract：:Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...

journal_title：Journal of bacteriology

authors： Silbert DF,Ruch F,Vagelos PR

更新日期：1968-05-01 00:00:00