Induction and repression of nitrate reductase in Neurospora crassa.

Abstract:

:Synthesis of wild-type Neurospora crassa assimilatory nitrate reductase is induced in the presence of nitrate ions and repressed in the presence of ammonium ions. Effects of several Neurospora mutations on the regulation of this enzyme are shown: (i) the mutants, nit-1 and nit-3, involving separate lesions, lack reduced nicotinamide adenine dinucleotide (NADPH)-nitrate reductase activity and at least one of three other activities associated with the wild-type enzyme. The two mutants do not require the presence of nitrate for induction of their aberrant nitrate reductases and are constitutive for their component nitrate reductase activities in the absence of ammonium ions. (ii) An analog of the wild-type enzyme (similar to the nit-1 enzyme) is formed when wild type is grown in a medium in which molybdenum has been replaced by vanadium or tungsten; the resulting enzyme lacks NADPH-nitrate reductase activity. Unlike nit-1, wild type produced this analog only in the presence of nitrate. Contaminating nitrate does not appear to be responsible for the observed mutants' activities. Nitrate reductase is proposed to be autoregulated. (iii) Mutants (am) lacking NADPH-dependent glutamate dehydrogenase activity partially escape ammonium repression of nitrate reductase. The presence of nitrate is required for the enzyme's induction. (iv) A double mutant, nit-1 am-2, proved to be an ideal test system to study the repressive effects of nitrogen-containing metabolites on the induction of nitrate reductase activity. The double mutant does not require nitrate for induction of nitrate reductase, and synthesis of the enzyme is not repressed by the presence of high concentrations of ammonium ions. It is, however, repressed by the presence of any one of six amino acids. Nitrogen metabolites (other than ammonium) appear to be responsible for the mediation of "ammonium repression."

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Dantzig AH,Zurowski WK,Ball TM,Nason A

doi

10.1128/JB.133.2.671-679.1978

keywords:

subject

Has Abstract

pub_date

1978-02-01 00:00:00

pages

671-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

133

pub_type

杂志文章
  • The gene encoding the glutamate dehydrogenase in Lactococcus lactis is part of a remnant Tn3 transposon carried by a large plasmid.

    abstract::The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.14.5019-5022.2005

    authors: Tanous C,Chambellon E,Sepulchre AM,Yvon M

    更新日期:2005-07-01 00:00:00

  • Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci.

    abstract::Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.2.169-180.1961

    authors: FIRSHEIN W

    更新日期:1961-08-01 00:00:00

  • FK-506-binding proteins from streptomycetes producing immunosuppressive macrolactones of the FK-506 type.

    abstract::FK-506-binding proteins (FKBPs), which in T cells are supposed to mediate the immunosuppressive effects of the compounds FK-506 and rapamycin, have been isolated from Streptomyces chrysomallus, S. hygroscopicus subsp. ascomyceticus, and S. hygroscopicus. The latter two strains are producers of ascomycin (the ethyl ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.18.5888-5894.1992

    authors: Pahl A,Keller U

    更新日期:1992-09-01 00:00:00

  • In vivo-selected mutations in methyl-directed mismatch repair suppress the virulence attenuation of Salmonella dam mutant strains following intraperitoneal, but not oral, infection of naïve mice.

    abstract::Salmonella enterica serovar Typhimurium that lacks the DNA adenine methylase (Dam) ectopically expresses multiple genes that are preferentially expressed during infection, is attenuated for virulence, and confers heightened immunity in vaccinated hosts. The safety of dam mutant Salmonella vaccines was evaluated by scr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00299-07

    authors: Heithoff DM,Badie G,Julio SM,Enioutina EY,Daynes RA,Sinsheimer RL,Mahan MJ

    更新日期:2007-07-01 00:00:00

  • Characterization of a newly identified mycoplasma (Mycoplasma orale type 3) from the human oropharynx.

    abstract::Six mycoplasma strains, isolated under anaerobic conditions from the human oropharynx, were studied by biologic and serologic means. The strains produced nippled colonies with weak hemolytic activity for guinea pig erythrocytes on agar medium. In addition, the strains metabolized arginine with a concomitant alkaline s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.36-43.1969

    authors: Fox H,Purcell RH,Chanock RM

    更新日期:1969-04-01 00:00:00

  • Physiological effects of Crl in Salmonella are modulated by sigmaS level and promoter specificity.

    abstract::The small regulatory protein Crl activates sigma(S) (RpoS), the stationary-phase and general stress response sigma factor. Crl has been reported to bind sigma(S) in vitro and to facilitate the formation of RNA polymerase holoenzyme. In Salmonella enterica serovar Typhimurium, Crl is required for the development of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01919-06

    authors: Robbe-Saule V,Lopes MD,Kolb A,Norel F

    更新日期:2007-04-01 00:00:00

  • Enzymatic specificities and modes of action of the two catalytic domains of the XynC xylanase from Fibrobacter succinogenes S85.

    abstract::The xylanase XynC of Fibrobacter succinogenes S85 was recently shown to contain three distinct domains, A, B, and C (F. W. Paradis, H. Zhu, P. J. Krell, J. P. Phillips, and C. W. Forsberg, J. Bacteriol. 175:7666-7672, 1993). Domains A and B each bear an active site capable of hydrolyzing xylan, while domain C has no e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.13.3885-3894.1994

    authors: Zhu H,Paradis FW,Krell PJ,Phillips JP,Forsberg CW

    更新日期:1994-07-01 00:00:00

  • Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease.

    abstract::Mushin, Rose (University of Melbourne, Victoria, Australia) and Frances M. Ashburner. Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease. J. Bacteriol. 83:1260-1267. 1962.-The aerobic bacterial intestinal flora of mutton birds, healthy and affected with "limy" disease, wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1260-1267.1962

    authors: MUSHIN R,ASHBURNER FM

    更新日期:1962-06-01 00:00:00

  • Mutations at several loci cause increased expression of ribonucleotide reductase in Escherichia coli.

    abstract::Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05989-11

    authors: Feeney MA,Ke N,Beckwith J

    更新日期:2012-03-01 00:00:00

  • Phospholipid turnover in a conditional polyamine auxotroph of Escherichia coli.

    abstract::Polyamines are required for rapid phospholipid turnover in Escherichia coli growing in media of low osmolarity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1479-1481.1973

    authors: Munro GF,Bell CA

    更新日期:1973-12-01 00:00:00

  • DRUG RESISTANCE OF ENTERIC BACTERIA. II. TRANSDUCTION OF TRANSMISSIBLE DRUG-RESISTANCE (R) FACTORS WITH PHAGE EPSILON.

    abstract::Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. II. Transduction of transmissible drug-resistance (R) factors with phage epsilon. J. Bacteriol. 86:1332-1338. 1963.-Transmissible drug-resistance (R) factors, which transfer resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1332-1338.1963

    authors: HARADA K,KAMEDA M,SUZUKI M,MITSUHASHI S

    更新日期:1963-12-01 00:00:00

  • Chromosomal mutation that affects excretion of hemolysin in Escherichia coli.

    abstract::Two types of mutants unable to excrete hemolysin were obtained when E. coli 5K carrying the multicopy hemolytic recombinant plasmid pANN202-312 was mutagenized with Mu d1. One type is altered in the plasmid hly-specific gene, hlyBb, but the other is caused by an insertion of Mu d1 into a chromosomal locus. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.3.1083-1085.1984

    authors: Juarez A,Goebel W

    更新日期:1984-09-01 00:00:00

  • Impact of Membrane Phospholipid Alterations in Escherichia coli on Cellular Function and Bacterial Stress Adaptation.

    abstract::Bacteria have evolved multiple strategies to sense and rapidly adapt to challenging and ever-changing environmental conditions. The ability to alter membrane lipid composition, a key component of the cellular envelope, is crucial for bacterial survival and adaptation in response to environmental stress. However, the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00849-16

    authors: Rowlett VW,Mallampalli VKPS,Karlstaedt A,Dowhan W,Taegtmeyer H,Margolin W,Vitrac H

    更新日期:2017-06-13 00:00:00

  • Autoplaquing in Myxococcus strains.

    abstract::Autoplaquing has been observed in Myxococcus strains freshly isolated from soil. Initial observations suggest that this phenomenon is not induced by elevated temperature or visible light; we suggest that it may be the result of a derangement in the developmental autolytic mechanism within the cell. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.1163-1164.1984

    authors: Breyen SA,Dworkin M

    更新日期:1984-06-01 00:00:00

  • Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters.

    abstract::The glnB gene from Bradyrhizobium japonicum, the endosymbiont of soybeans (Glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. The B. japonicum glnB gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnB gene product...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5638-5645.1989

    authors: Martin GB,Thomashow MF,Chelm BK

    更新日期:1989-10-01 00:00:00

  • Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli.

    abstract::Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.2.545-555.1976

    authors: Kumar S

    更新日期:1976-02-01 00:00:00

  • Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway.

    abstract::We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.111-118.2002

    authors: Tian H,Beckwith J

    更新日期:2002-01-01 00:00:00

  • ZmaR, a novel and widespread antibiotic resistance determinant that acetylates zwittermicin A.

    abstract::ZmaR is a resistance determinant of unusual abundance in the environment and confers on gram-positive and gram-negative bacteria resistance to zwittermicin A, a novel broad-spectrum antibiotic produced by species of Bacillus. The ZmaR protein has no sequence similarity to proteins of known function; thus, the purpose ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.17.5455-5460.1999

    authors: Stohl EA,Brady SF,Clardy J,Handelsman J

    更新日期:1999-09-01 00:00:00

  • Analysis of Halobacterium halobium gas vesicles.

    abstract::Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.3.1058-1067.1973

    authors: Krantz MJ,Ballou CE

    更新日期:1973-06-01 00:00:00

  • Regulation at the phosphoenolpyruvate branchpoint in Azotobacter vinelandii: phosphoenolpyruvate carboxylase.

    abstract::Phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.1.31-36.1971

    authors: Liao CL,Atkinson DE

    更新日期:1971-04-01 00:00:00

  • Replication of a plasmid lacking the normal site for initiation of one strand.

    abstract::The origin of replication of the plasmid R1162 contains an initiation site for the synthesis of each DNA strand. When one of these sites (oriL) is deleted, synthesis on the corresponding strand is no longer initiated efficiently in vitro by the R1162-encoded replication proteins, and the plasmid is no longer stably ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.16.4870-4876.1996

    authors: Becker EC,Zhou H,Meyer RJ

    更新日期:1996-08-01 00:00:00

  • Molecular cloning and transcriptional analysis of a guanosine kinase gene of Brevibacterium acetylicum ATCC 953.

    abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.6959-6964.1997

    authors: Usuda Y,Kawasaki H,Shimaoka M,Utagawa T

    更新日期:1997-11-01 00:00:00

  • Initiation of swarming motility by Proteus mirabilis occurs in response to specific cues present in urine and requires excess L-glutamine.

    abstract::Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02136-12

    authors: Armbruster CE,Hodges SA,Mobley HL

    更新日期:2013-03-01 00:00:00

  • Effect of alanine, leucine and fructose on lysyl-transfer ribonucleic acid ligase activity in a mutant of Escherichia coli K-12.

    abstract::A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.167-177.1973

    authors: Hirshfield IN,Bukald NE

    更新日期:1973-01-01 00:00:00

  • Role of lithium ions in proline transport in Escherichia coli.

    abstract::Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.560-564.1979

    authors: Kayama-Gonda Y,Kawasaki T

    更新日期:1979-08-01 00:00:00

  • Purification, characterization, and physiological function of Bacillus subtilis ornithine transcarbamylase.

    abstract::A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.512-521.1983

    authors: Neway JO,Switzer RL

    更新日期:1983-08-01 00:00:00

  • ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification.

    abstract::Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.3.1245-1253.1988

    authors: Grodberg J,Dunn JJ

    更新日期:1988-03-01 00:00:00

  • Replication Rapidly Recovers and Continues in the Presence of Hydroxyurea in Escherichia coli.

    abstract::In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00713-17

    authors: Nazaretyan SA,Savic N,Sadek M,Hackert BJ,Courcelle J,Courcelle CT

    更新日期:2018-02-23 00:00:00

  • Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells.

    abstract::Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.300-308.1971

    authors: Levy SB

    更新日期:1971-10-01 00:00:00

  • THE GENUS VEILLONELLA. I. GENERAL CULTURAL, ECOLOGICAL, AND BIOCHEMICAL CONSIDERATIONS.

    abstract::Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.162-170.1964

    authors: ROGOSA M

    更新日期:1964-01-01 00:00:00