Nature of the complementation products formed by a complementing mutant of neurospora crassa.

abstract：:The predicted amino acid sequence of Bacillus subtilis ycsK exhibits similarity to the GDSL family of lipolytic enzymes. Northern blot analysis showed that ycsK mRNA was first detected from 4 h after the onset of sporulation and that transcription of ycsK was dependent on SigK and GerE. The fluorescence of the YcsK-gr...

journal_title：Journal of bacteriology

authors： Masayama A,Kuwana R,Takamatsu H,Hemmi H,Yoshimura T,Watabe K,Moriyama R

更新日期：2007-03-01 00:00:00

abstract：:Methylation of DNA at the C-5 position of cytosine occurs in diverse organisms. This modification can increase the rate of C→T transitions at the methylated position. In Escherichia coli and related enteric bacteria, the inner C residues of the sequence CCWGG (W is A or T) are methylated by the Dcm enzyme. These sites...

journal_title：Journal of bacteriology

authors： Cherry JL

更新日期：2018-11-26 00:00:00

abstract：:The gene encoding the major NAD(P)H-flavin oxidoreductase (flavin reductase) of the luminous bacterium Vibrio fischeri ATCC 7744 was isolated by using synthetic oligonucleotide probes corresponding to the N-terminal amino acid sequence of the enzyme. Nucleotide sequence analysis suggested that the major flavin reducta...

journal_title：Journal of bacteriology

authors： Zenno S,Saigo K,Kanoh H,Inouye S

更新日期：1994-06-01 00:00:00

abstract：:The distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species was examined. Enzyme activity was demonstrated in cell-free growth media from all members of the family Vibrionaceae which were tested except Plesiomonas shigelloides. In each case, enzyme was produced in exponential to e...

journal_title：Journal of bacteriology

authors： MacIntyre S,Trust TJ,Buckley JT

更新日期：1979-07-01 00:00:00

abstract：:Haloarchaea in their natural environment are exposed to hypersalinity, intense solar radiation, and desiccation, all of which generate high levels of oxidative stress. Previous work has shown that haloarchaea are an order of magnitude more resistant to oxidative stress than most mesophilic organisms. Despite this resi...

journal_title：Journal of bacteriology

authors： Gelsinger DR,DiRuggiero J

更新日期：2018-04-09 00:00:00

abstract：:The SsuDAT1I restriction-modification (R-M) system, which contains two methyltransferases and two restriction endonucleases with recognition sequence 5'-GATC-3', was first found in a field isolate of Streptococcus suis serotype 2. Isoschizomers of the R-M system were found in the same locus between purH and purD in a ...

journal_title：Journal of bacteriology

authors： Sekizaki T,Osaki M,Takamatsu D,Shimoji Y

更新日期：2001-09-01 00:00:00

abstract：:Bacillus subtilis gene ypfP, which is located at 196 degrees on the genetic map, shows similarity to both the monogalactosyldiacylglycerol synthase gene of Cucumis sativus, which encodes a galactosyltransferase, and the murG genes of B. subtilis, Escherichia coli, Haemophilus influenzae, and Synechocystis sp. strain P...

journal_title：Journal of bacteriology

authors： Price KD,Roels S,Losick R

更新日期：1997-08-01 00:00:00

abstract：:Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

journal_title：Journal of bacteriology

authors： Madigan MT,Brock TD

更新日期：1975-05-01 00:00:00

abstract：:In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...

journal_title：Journal of bacteriology

authors： Pianzzola MJ,Soubes M,Touati D

更新日期：1996-12-01 00:00:00

abstract：:Bulk membrane fragments were prepared from cells of Bacillus cereus ATCC 4342 harvested at different stages of growth and sporulation and examined for enzymes involved in electron transport functions. The presence of succinate: DCPIP oxidoreductase (EC 1.3.99.1), succinate: cytochrome c oxidoreductase (EC 1.3.2.1), NA...

journal_title：Journal of bacteriology

authors： Lang DR,Felix J,Lundgren DG

更新日期：1972-06-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

journal_title：Journal of bacteriology

authors： Gardner RM,Doerner KC,White BA

更新日期：1987-10-01 00:00:00

abstract：:The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...

journal_title：Journal of bacteriology

authors： Suzuki K,Wang X,Weilbacher T,Pernestig AK,Melefors O,Georgellis D,Babitzke P,Romeo T

更新日期：2002-09-01 00:00:00

abstract：:An in vitro system of cell envelopes from Salmonella typhimurium with functional flagella was used to determine the minimal requirements for flagellar rotation. Rotation in the absence of cytoplasmic constituents could be driven either by respiration or by an artificially imposed chemical gradient of protons. No speci...

journal_title：Journal of bacteriology

authors： Ravid S,Eisenbach M

更新日期：1984-06-01 00:00:00

abstract：:In many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. We report the characterization of a gene coding for an alkane hydroxylase in a Burkholderia cepacia strain isolated from an oil-contaminated site. The protein encoded showed similarity to ot...

journal_title：Journal of bacteriology

authors： Marín MM,Smits TH,van Beilen JB,Rojo F

更新日期：2001-07-01 00:00:00

abstract：:Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

journal_title：Journal of bacteriology

authors： Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

更新日期：2002-07-01 00:00:00

abstract：:Spirochetes are an evolutionary and structurally unique group of bacteria. Outermost is a membrane sheath (OS), and within this sheath are the protoplasmic cell cylinder (PC) and periplasmic flagella (PFs). The PFs are attached at each end of the PC and, depending on the species, may or may not overlap in the center o...

journal_title：Journal of bacteriology

authors： Goldstein SF,Buttle KF,Charon NW

更新日期：1996-11-01 00:00:00

abstract：:Plasmids pRAS3.1 and pRAS3.2 are natural variants of the IncQ-2 plasmid family, that except for two differences, have identical plasmid backbones. Plasmid pRAS3.1 has four 22-bp iterons in its oriV region, while pRAS3.2 has only three 6-bp repeats and pRAS3.1 has five 6-bp repeats in the promoter region of the mobB-mo...

journal_title：Journal of bacteriology

authors： Loftie-Eaton W,Rawlings DE

更新日期：2010-12-01 00:00:00

abstract：:The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine prot...

journal_title：Journal of bacteriology

authors： Aizawa SI,Dean GE,Jones CJ,Macnab RM,Yamaguchi S

更新日期：1985-03-01 00:00:00

abstract：:Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns. A group of the tested bacteria (group A), Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus, was...

journal_title：Journal of bacteriology

authors： Iwasaki H,Shimada A,Ito E

更新日期：1986-08-01 00:00:00

abstract：:Replication-proficient (Rep+) revertants were isolated from mutants of IncFII plasmid NR1 that were replication defective (Rep-). The parental Rep- plasmids contained a mutation that inactivated promoter PE for transcription of RNA-E, a trans-acting repressor of translation of the essential RepA1 replication initiatio...

journal_title：Journal of bacteriology

authors： Wu R,Wang X,Womble DD,Rownd RH

更新日期：1993-05-01 00:00:00

abstract：:We have previously shown that both ends of the Tn3 family transposon Tn4652 contain integration host factor (IHF) binding sites and that IHF positively regulates expression of the Tn4652 transposase gene tnpA in Pseudomonas putida (R. Hõrak, and M. Kivisaar, J. Bacteriol. 180:2822-2829, 1998). Tn4652 can activate sile...

journal_title：Journal of bacteriology

authors： Teras R,Hõrak R,Kivisaar M

更新日期：2000-02-01 00:00:00

abstract：:Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...

journal_title：Journal of bacteriology

authors： Vinopal RT,Fraenkel DG

更新日期：1974-06-01 00:00:00

abstract：:Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

journal_title：Journal of bacteriology

authors： Stern ML,Phillips AW,Gottlieb AJ

更新日期：1976-02-01 00:00:00

abstract：:The skin represents an important barrier for pathogens and is known to produce fatty acids that are toxic toward gram-positive bacteria. A screen of fatty acids as growth inhibitors of Staphylococcus aureus revealed structure-specific antibacterial activity. Fatty acids like oleate (18:1Δ9) were nontoxic, whereas palm...

journal_title：Journal of bacteriology

authors： Parsons JB,Yao J,Frank MW,Jackson P,Rock CO

更新日期：2012-10-01 00:00:00

abstract：:Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...

journal_title：Journal of bacteriology

authors： Cerda-Maira FA,Ringelberg CS,Taylor RK

更新日期：2008-11-01 00:00:00

abstract：:The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...

journal_title：Journal of bacteriology

authors： Huh WK,Kang SO

更新日期：1999-07-01 00:00:00

abstract：:Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patter...

journal_title：Journal of bacteriology

authors： Dale JW,Al-Ghusein H,Al-Hashmi S,Butcher P,Dickens AL,Drobniewski F,Forbes KJ,Gillespie SH,Lamprecht D,McHugh TD,Pitman R,Rastogi N,Smith AT,Sola C,Yesilkaya H

更新日期：2003-04-01 00:00:00

abstract：:Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

journal_title：Journal of bacteriology

authors： Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

更新日期：2007-04-01 00:00:00

abstract：:Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...

journal_title：Journal of bacteriology

authors： Chang YF,Feingold DS

更新日期：1969-09-01 00:00:00