Host-induced modification-restriction and the utilization of 5-bromouracil by thymineless mutants of Escherichia coli.

abstract：:Heterocysts isolated from Anabaena sp. strain 7120 with lysozyme plus sonication were permeabilized with the cationic detergent cetyltrimethylammonium bromide, and they then exhibited comparable acetylene reduction activity in the light and dark with an ATP-regenerating system plus dithionite. The detergent diminished...

journal_title：Journal of bacteriology

authors： Privalle LS,Burris RH

更新日期：1983-08-01 00:00:00

abstract：:The conditions for the infection of Bacillus stearothermophilus 4S with TP-1C phage deoxyribonucleic acid (DNA) are described. Cells from log-phase cultures are the most susceptible to phage DNA infection (transfection). A cellular component (competence factor) which enhances transfection is released into the culture ...

journal_title：Journal of bacteriology

authors： Streips UN,Welker NE

更新日期：1971-06-01 00:00:00

abstract：:An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...

journal_title：Journal of bacteriology

authors： Li T,Rosazza JP

更新日期：1997-06-01 00:00:00

abstract：:Fructose uptake and catabolism in Azospirillum brasilense is dependent on three fructose-inducible enzymes (fru-enzymes): (i) enzyme I and (ii) enzyme II of the phosphoenolpyruvate:fructose phosphotransferase system and (iii) 1-phosphofructokinase. In minimal medium containing 3.7 mM succinate and 22 mM fructose as so...

journal_title：Journal of bacteriology

authors： Mukherjee A,Ghosh S

更新日期：1987-09-01 00:00:00

abstract：:Eight species representative of the serological diversity of the Mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. Cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (Eagle). For Acholeplasma laidl...

journal_title：Journal of bacteriology

authors： McIvor RS,Kenny GE

更新日期：1978-08-01 00:00:00

abstract：:Type 1 fimbriae and flagella have been previously shown to contribute to the virulence of uropathogenic Escherichia coli (UPEC) within the urinary tract. In this study, the relationship between motility and type 1 fimbrial expression was tested for UPEC strain CFT073 by examining the phenotypic effect of fimbrial expr...

journal_title：Journal of bacteriology

authors： Lane MC,Simms AN,Mobley HL

更新日期：2007-08-01 00:00:00

abstract：:Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...

journal_title：Journal of bacteriology

authors： Ruppert C,Wimmers S,Lemker T,Müller V

更新日期：1998-07-01 00:00:00

abstract：:The gene encoding the major NAD(P)H-flavin oxidoreductase (flavin reductase) of the luminous bacterium Vibrio fischeri ATCC 7744 was isolated by using synthetic oligonucleotide probes corresponding to the N-terminal amino acid sequence of the enzyme. Nucleotide sequence analysis suggested that the major flavin reducta...

journal_title：Journal of bacteriology

authors： Zenno S,Saigo K,Kanoh H,Inouye S

更新日期：1994-06-01 00:00:00

abstract：:Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...

journal_title：Journal of bacteriology

authors： Grogan DW,Rockwood J

更新日期：2010-09-01 00:00:00

abstract：:Vibrio vulnificus is a human pathogen that produces lethal septicemia in susceptible persons, and the primary virulence factor for this organism is capsular polysaccharide (CPS). The role of the capsule in V. vulnificus biofilms was examined under a variety of conditions, by using either defined CPS mutants or spontan...

journal_title：Journal of bacteriology

authors： Joseph LA,Wright AC

更新日期：2004-02-01 00:00:00

abstract：:The DnaA protein concentration in Escherichia coli was increased above the wild-type level by inducing a lacP-controlled dnaA gene located on a plasmid. In these cells with different DnaA protein levels, we measured several parameters: dnaA gene expression; cell size, amount of DNA per cell, and number of origins per ...

journal_title：Journal of bacteriology

authors： Atlung T,Hansen FG

更新日期：1993-10-01 00:00:00

abstract：:Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...

journal_title：Journal of bacteriology

authors： Crowe BA,Owen P

更新日期：1983-03-01 00:00:00

abstract：:We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in th...

journal_title：Journal of bacteriology

authors： Duthy TG,Staendner LH,Manning PA,Heuzenroeder MW

更新日期：1999-09-01 00:00:00

abstract：:The allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. The implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organ...

journal_title：Journal of bacteriology

authors： Jensen RA,Stenmark SL

更新日期：1970-03-01 00:00:00

abstract：:The urease activity of Ureaplasma urealyticum was found to be located in the cytoplasmic fraction and consisted of multiple stable enzyme forms representing at least four biotypes. ...

journal_title：Journal of bacteriology

authors： Delisle GJ

更新日期：1977-06-01 00:00:00

abstract：:The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

journal_title：Journal of bacteriology

authors： Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

更新日期：1990-05-01 00:00:00

abstract：:Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...

journal_title：Journal of bacteriology

authors： Levy SB

更新日期：1971-10-01 00:00:00

abstract：:Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

journal_title：Journal of bacteriology

authors： Coyne MJ,Fletcher CM,Reinap B,Comstock LE

更新日期：2011-10-01 00:00:00

abstract：:Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...

journal_title：Journal of bacteriology

authors： Price LB,Shand RF

更新日期：2000-09-01 00:00:00

abstract：:Infection of Escherichia coli with amber mutants of phage fd, in contrast to infection with wild-type phage, leads to cell death and the proliferation of intracytoplasmic membranes observed in electron micrographs at the poles of the cells. The accumulation of membranes correlates with changes in structural phospholip...

journal_title：Journal of bacteriology

authors： Onishi Y

更新日期：1971-09-01 00:00:00

abstract：:Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...

journal_title：Journal of bacteriology

authors： Wang X,Gao Z,Xu X,Ruan L

更新日期：2011-10-01 00:00:00

abstract：:Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Detection and persistence of Vi antigen in tissues of actively immunized mice. J. Bacteriol. 89:776-781. 1965.-The presence, distribution, and persistence of Vi antigen in mouse tissue was determined by me...

journal_title：Journal of bacteriology

authors： GAINES S,CURRIE JA,TULLY JG

更新日期：1965-03-01 00:00:00

abstract：:The antibiotic cerulenin causes the immediate cessation of phospholipid biosynthesis in both chemoheterotrophic and photoheterotrophic cultures of Rhodopseudomonas sphaeroides. Macromolecule biosynthesis in photoheterotrophic cells was unaffected by cerulenin for the first 2 h after antibiotic addition and then contin...

journal_title：Journal of bacteriology

authors： Shepherd WD,Kaplan S

更新日期：1983-12-01 00:00:00

abstract：:Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...

journal_title：Journal of bacteriology

authors： Sebulsky MT,Hohnstein D,Hunter MD,Heinrichs DE

更新日期：2000-08-01 00:00:00

abstract：:The ability of Clostridium perfringens type A to produce an enterotoxin active in human food poisoning has been shown to be directly related to the ability of the organism to sporulate. Enterotoxin was produced only in a sporulation medium and not in a growth medium in which sporulation was repressed. Mutants with an ...

journal_title：Journal of bacteriology

authors： Duncan CL,Strong DH,Sebald M

更新日期：1972-04-01 00:00:00

abstract：:Gill lamellae from the young fruiting bodies of the basidiomycete Coprinus micaceus possess cytoplasmic particles with cytochemically demonstrable acid phosphatase activity; they are presumed to be lysosomes. ...

journal_title：Journal of bacteriology

authors： Nehemiah JL

更新日期：1973-07-01 00:00:00

abstract：:We previously described a cell surface anionic polysaccharide (APS) in Porphyromonas gingivalis that is required for cell integrity and serum resistance. APS is a phosphorylated branched mannan that shares a common epitope with posttranslational additions to some of the Arg-gingipains. This study aimed to determine th...

journal_title：Journal of bacteriology

authors： Rangarajan M,Aduse-Opoku J,Paramonov N,Hashim A,Bostanci N,Fraser OP,Tarelli E,Curtis MA

更新日期：2008-04-01 00:00:00

abstract：:Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival...

journal_title：Journal of bacteriology

authors： ELDER RL,BEERS RF Jr

更新日期：1965-05-01 00:00:00

abstract：:Two bacteriophage BF23 late genes, genes 24 and 25, were isolated on a 7.4-kb PstI fragment from the phage DNA, and their nucleotide sequences were determined. Gene 24 encodes a minor tail protein with the expected M(r) of 34,309, and gene 25 located 4 bp upstream of gene 24 encodes a major tail protein with the expec...

journal_title：Journal of bacteriology

authors： Nakayama S,Kaneko T,Ishimaru H,Moriwaki H,Mizobuchi K

更新日期：1994-12-01 00:00:00

abstract：:A rough (R) Brucella abortus 45/20 mutant was more sensitive to the bactericidal activity of polymyxin B and lactoferricin B than was its smooth (S) counterpart but considerably more resistant than Salmonella montevideo. The outer membrane (OM) and isolated lipopolysaccharide (LPS) of S. montevideo showed a higher aff...

journal_title：Journal of bacteriology

authors： Freer E,Moreno E,Moriyón I,Pizarro-Cerdá J,Weintraub A,Gorvel JP

更新日期：1996-10-01 00:00:00