Effect of cerulenin on macromolecule synthesis in chemoheterotrophically and photoheterotrophically grown Rhodopseudomonas sphaeroides.

abstract：:A detailed study of the cultural characteristics and cellular fatty acid composition of 27 isolates of Corynebacterium acnes was performed to establish the properties by which this organism may be identified and characterized. The fatty acids were extracted directly from whole cells and examined as methyl esters by ga...

journal_title：Journal of bacteriology

authors： Moss CW,Dowell VR Jr,Lewis VJ,Schekter MA

更新日期：1967-11-01 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) quorum sensing is common to many Proteobacteria including a clinical isolate of Burkholderia cepacia. The B. cepacia isolate produces low levels of octanoyl-HSL. We have examined an environmental isolate of Burkholderia vietnamiensis. This isolate produced several acyl-HSLs. The most...

journal_title：Journal of bacteriology

authors： Conway BA,Greenberg EP

更新日期：2002-02-01 00:00:00

abstract：:Cohen, Jay O. (Communicable Disease Center, Atlanta, Ga.), Glenda S. Cowart, and William B. Cherry. Antibodies against Staphylococcus aureus in nonimmunized rabbits. J. Bacteriol. 82:110-114. 1961.-Antibody against staphylococci was demonstrated in the serum of each of 36 nonimmunized specific-pathogen-free rabbits th...

journal_title：Journal of bacteriology

authors： COHEN JO,COWART GS,CHERRY WB

更新日期：1961-07-01 00:00:00

abstract：:A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinat...

journal_title：Journal of bacteriology

authors： Edwards DL,Belsole DM,Guzik HJ,Unger BW

更新日期：1979-02-01 00:00:00

abstract：:Streptomyces venezuelae ATCC 10595 harbors seven rRNA gene clusters which can be distinguished by BglII digestion. The three rRNA genes present in each set are closely linked with the general structure 16S-23S-5S. We cloned rrnA and sequenced the 16S-23S spacer region and the region downstream of the 5S rRNA gene. No ...

journal_title：Journal of bacteriology

authors： La Farina M,Stira S,Mancuso R,Grisanti C

更新日期：1996-03-01 00:00:00

abstract：:Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

journal_title：Journal of bacteriology

authors： Fitz-James PC

更新日期：1971-03-01 00:00:00

abstract：:The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...

journal_title：Journal of bacteriology

authors： Haddad JE,al-Jaufy AY,Jackson MP

更新日期：1993-08-01 00:00:00

abstract：:Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pa...

journal_title：Journal of bacteriology

authors： Mowa MB,Warner DF,Kaplan G,Kana BD,Mizrahi V

更新日期：2009-02-01 00:00:00

abstract：:Strains of Caulobacter crescentus elaborate an S-layer, a two-dimensional protein latticework which covers the cell surface. The S-layer protein (RsaA) is secreted by a type I mechanism (relying on a C-terminal signal) and is unusual among type I secreted proteins because high levels of protein are produced continuous...

journal_title：Journal of bacteriology

authors： Umelo-Njaka E,Bingle WH,Borchani F,Le KD,Awram P,Blake T,Nomellini JF,Smit J

更新日期：2002-05-01 00:00:00

abstract：:By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...

journal_title：Journal of bacteriology

authors： Vermeulen CA,Venema G

更新日期：1974-05-01 00:00:00

abstract：:Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS). Earlier studies indicated that an alternate glucose transport system functions in this organism under conditions of high growth rates, low pH, or excess glucose. To identify this system, S. mutans...

journal_title：Journal of bacteriology

authors： Cvitkovitch DG,Boyd DA,Thevenot T,Hamilton IR

更新日期：1995-05-01 00:00:00

abstract：:Protoplasts of Saccharomyces cerevisiae were mixed with linear DNA plasmids, pGKl1 and pGKl2, isolated from a Kluyveromyces lactis killer strain and treated with polyethylene glycol. Out of 2,000 colonies regenerated on a nonselective medium, two killer transformants were obtained. The pGKl plasmids and the killer cha...

journal_title：Journal of bacteriology

authors： Gunge N,Murata K,Sakaguchi K

更新日期：1982-07-01 00:00:00

abstract：:We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and al...

journal_title：Journal of bacteriology

authors： Ou JT,Anderson TF

更新日期：1970-06-01 00:00:00

abstract：:DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. Dna...

journal_title：Journal of bacteriology

authors： Bonilla CY,Grossman AD

更新日期：2012-09-01 00:00:00

abstract：:Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ...

journal_title：Journal of bacteriology

authors： ENGLESBERG E,ANDERSON RL,WEINBERG R,LEE N,HOFFEE P,HUTTENHAUER G,BOYER H

更新日期：1962-07-01 00:00:00

abstract：:We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extra...

journal_title：Journal of bacteriology

authors： Calvin NM,Hanawalt PC

更新日期：1988-06-01 00:00:00

abstract：:The anaerobic pathogen Clostridium perfringens encodes either toxin genes or antibiotic resistance determinants on a unique family of conjugative plasmids that have a novel conjugation region, the tcp locus. Studies of the paradigm conjugative plasmid from C. perfringens, the 47-kb tetracycline resistance plasmid pCW3...

journal_title：Journal of bacteriology

authors： Wisniewski JA,Teng WL,Bannam TL,Rood JI

更新日期：2015-02-15 00:00:00

abstract：:Integrons facilitate the evolution of complex phenotypes by physical and transcriptional linkage of genes. They can be categorized as chromosomal integrons (CIs) or mobile resistance integrons (MRIs). The significance of MRIs for the problem of multiple antibiotic resistance is well established. CIs are more widesprea...

journal_title：Journal of bacteriology

authors： Tetu SG,Holmes AJ

更新日期：2008-07-01 00:00:00

abstract：:A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...

journal_title：Journal of bacteriology

authors： Brandner JP,McEwan AG,Kaplan S,Donohue TJ

更新日期：1989-01-01 00:00:00

abstract：:The lprG-Rv1410c operon is critical for the survival of Mycobacterium tuberculosis during infection, but very little is known about the functions of its proteins. LprG is a lipoprotein, and Rv1410c encodes the major facilitator superfamily small molecule transporter P55. P55 likely exports small molecules outside of t...

journal_title：Journal of bacteriology

authors： Farrow MF,Rubin EJ

更新日期：2008-03-01 00:00:00

abstract：:Intact cells of Bacillus stearothermophilus PV72 revealed, after conventional thin-sectioning procedures, the typical cell wall profile of S-layer-carrying gram-positive eubacteria consisting of a ca. 10-nm-thick peptidoglycan-containing layer and a ca. 10-nm-thick S layer. Cell wall preparations obtained by breaking ...

journal_title：Journal of bacteriology

authors： Breitwieser A,Gruber K,Sleytr UB

更新日期：1992-12-01 00:00:00

abstract：:The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...

journal_title：Journal of bacteriology

authors： Every D,Skerman TM

更新日期：1980-02-01 00:00:00

abstract：:Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...

journal_title：Journal of bacteriology

authors： Burke WF Jr,Slinker BS

更新日期：1982-02-01 00:00:00

abstract：:The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

journal_title：Journal of bacteriology

authors： Sundaram TK,Fincham JR

更新日期：1968-03-01 00:00:00

abstract：:Adenosine 5'-triphosphate (ATP) synthesis energized by an artificially imposed protonmotive force (delta p) in adenosine 5'-diphosphate-loaded membrane vesicles of Escherichia coli was investigated. The protonmotive force is composed of an artificially imposed pH gradient (delta pH) or membrane potential (deltapsi), o...

journal_title：Journal of bacteriology

authors： Tsuchiya T

更新日期：1977-02-01 00:00:00

abstract：:The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with deoxycytidylate deaminase and with the RIB7 gene of Saccharomyces cerevisiae. The ribG gene of B. subtilis and the ribD gene of ...

journal_title：Journal of bacteriology

authors： Richter G,Fischer M,Krieger C,Eberhardt S,Lüttgen H,Gerstenschläger I,Bacher A

更新日期：1997-03-01 00:00:00

abstract：:A number of catalase-deficient mutants of Escherichia coli which exhibit no assayable catalase activity were isolated. The only physiological difference between the catalase mutants and their parents was a 50- to 60-fold greater sensitivity to killing by hydrogen peroxide. For comparison, mutations in the xthA and rec...

journal_title：Journal of bacteriology

authors： Loewen PC

更新日期：1984-02-01 00:00:00

abstract：:Wall turnover was studied in Bacillus subtilis. The loss of radioactively labeled wall polymers was followed during exponential growth in batch and chemostat cultures. Turnover kinetics were identical under all growth conditions; pulse-labeled wall material was lost with first-order kinetics, but only after exponentia...

journal_title：Journal of bacteriology

authors： De Boer WR,Kruyssen FJ,Wouters JT

更新日期：1981-01-01 00:00:00

abstract：:Protein and ribonucleic acid (RNA) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. Immediately after heating, cells were incubated in growth media containing either [14C]leucine or [3H]uracil. The labeled cells were subsequently treated with pen...

journal_title：Journal of bacteriology

authors： Dean RG,McGroarty EJ

更新日期：1979-05-01 00:00:00

abstract：:A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...

journal_title：Journal of bacteriology

authors： Smith CJ

更新日期：1985-10-01 00:00:00