Abstract:
:We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extracellular spaces. The method has been used to transform bacterial cells with an efficiency greater than 10(9) transformants per microgram of plasmid. It has also been used to extract intact plasmid from transformed cells with efficiencies comparable to those of the traditional alkaline lysis or CsCl equilibrium density gradient techniques. The technique is simple and rapid, allowing a transformation or the preparation of microgram quantities of plasmid to be accomplished in minutes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Calvin NM,Hanawalt PCdoi
10.1128/jb.170.6.2796-2801.1988subject
Has Abstractpub_date
1988-06-01 00:00:00pages
2796-801issue
6eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.48-57.1972
更新日期:1972-10-01 00:00:00
abstract:UNLABELLED:Silica is deposited in and around the spore coat layer of Bacillus cereus, and enhances the spore's acid resistance. Several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). Homologous sequence search revealed a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00447-15
更新日期:2015-10-26 00:00:00
abstract::The effect of dihydrostreptomycin on the incorporation of amino acids into protein in antibiotic-deprived cells of a streptomycin-dependent strain of Escherichia coli B has been compared with its effect on protein synthesis in extracts from cells of the same strain. Stimulation of phenylalanine incorporation into prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.247-253.1969
更新日期:1969-10-01 00:00:00
abstract::The segrosome is the nucleoprotein complex that mediates accurate plasmid segregation. In addition to its multifunctional role in segrosome assembly, the ParG protein of multiresistance plasmid TP228 is a transcriptional repressor of the parFG partition genes. ParG is a homodimeric DNA binding protein, with C-terminal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01630-08
更新日期:2009-06-01 00:00:00
abstract::The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5127-5131.1992
更新日期:1992-08-01 00:00:00
abstract::Injection of mice with a sublethal dose of endotoxin 2 hr after administration of 0.1 ml of carbon tetrachloride (CCl(4)) killed 75% of the animals. CCl(4) alone killed no controls. Significant protection against this effect was afforded by 5 mg of cortisone and by 5 mg of nicotinamide adenine dinucleotide (NAD). With...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.6.1817-1823.1967
更新日期:1967-12-01 00:00:00
abstract::The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5197-5199.1988
更新日期:1988-11-01 00:00:00
abstract::Neely, W. Brock (The Dow Chemical Co., Midland, Mich.). Action of formaldehyde on microorganisms. I. Correlation of activity with formaldehyde metabolism. J. Bacteriol. 85:1028-1031. 1963.-The action of formaldehyde in concentrations sublethal for the culture appeared to be at least twofold. The first phase caused a d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.1028-1031.1963
更新日期:1963-05-01 00:00:00
abstract::Diazouracil (DZU) was shown to increase the mean cell volume of several bacterial species. The filaments which it induced with Escherichia coli B and Lactobacillus casei were examined in section by electron microscopy. In addition to the inhibition of division of E. coli by DZU, its effects were studied on mass increa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.416-425.1969
更新日期:1969-01-01 00:00:00
abstract::Products of the approximately 9.5-kb virB operon are proposed to direct the export of T-DNA/protein complexes across the Agrobacterium tumefaciens envelope en route to plant cells. The presence of conserved nucleoside triphosphate (NTP)-binding domains in VirB4 and VirB11 suggests that one or both proteins couple ener...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.6.1723-1734.1993
更新日期:1993-03-01 00:00:00
abstract::The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.4.877-882.1995
更新日期:1995-02-01 00:00:00
abstract::Nitrogenase activity in Rhodospirillum rubrum and in some other photosynthetic bacteria is regulated in part by the availability of light. This regulation is through a posttranslational modification system that is itself regulated by P(II) homologs in the cell. P(II) is one of the most broadly distributed regulatory p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00585-09
更新日期:2009-09-01 00:00:00
abstract::Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.17.5291-5294.1996
更新日期:1996-09-01 00:00:00
abstract::The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.759-765.1986
更新日期:1986-09-01 00:00:00
abstract::Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.788-795.1981
更新日期:1981-02-01 00:00:00
abstract::A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.495-501.1974
更新日期:1974-10-01 00:00:00
abstract::Gaspar, Andrew J. (Fort Detrick, Frederick, Md.), Hugh B. Tresselt, and Martha K. Ward. New solid medium for enhanced growth of Pasteurella tularensis. J. Bacteriol. 82:564-569. 1961.-The purpose of this work was to develop a solid medium for more rapid growth of Pasteurella tularensis, especially from small inocula c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.4.564-569.1961
更新日期:1961-10-01 00:00:00
abstract::A polysaccharide-rich particulate fraction was isolated from cytoplasmic extracts of Candida albicans by a procedure using differential centrifugation. The polysaccharide particles obtained after purification with deoxycholate treatment were essentially free of nitrogen and were identified chemically as polyglucosan, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.441-449.1974
更新日期:1974-10-01 00:00:00
abstract::Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepressio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5762-5764.1998
更新日期:1998-11-01 00:00:00
abstract::Salmonella typhimurium contains three "major proteins" or "porins" (34K, 35K, and 36K) in the outer membrane. A mutant strain producing only the 35K porin was first grown in media containing high concentrations of NaCl to "repress" the porin synthesis and then was shifted into a medium without NaCl. The newly made por...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.687-702.1978
更新日期:1978-08-01 00:00:00
abstract::The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.22.6948-6957.1999
更新日期:1999-11-01 00:00:00
abstract::An incompetent mutant (4S Com(-)) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.955-959.1971
更新日期:1971-06-01 00:00:00
abstract::The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive oxidase (CIO), aa3-type cytochrome c oxidase (aa3), and two cbb(3)-type cytochrome c oxidases (cbb(3)-1and cbb(3)-2). These terminal oxidases are differentially regulate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02176-14
更新日期:2014-12-01 00:00:00
abstract::Isolated sheaths of Leptothrix discophora SP-6 (ATCC 51168) were tested for susceptibility to degradation by a variety of chemical denaturants and lytic enzymes and found to be resistant to many reagents and enzyme treatments. However, disulfide bond-reducing agents such as dithiothreitol (DTT), beta-mercaptoethanol, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.7819-7827.1993
更新日期:1993-12-01 00:00:00
abstract::Exogenously supplied long-chain fatty acids have been shown to markedly alleviate the inhibition of phototrophic growth of cultures of Rhodopseudomonas sphaeroides caused by the antibiotic cerulenin. Monounsaturated and polyunsaturated C18 fatty acids were most effective in relieving growth inhibition mediated by ceru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.782-790.1983
更新日期:1983-02-01 00:00:00
abstract::Deoxyribonucleic acid (DNA), pulse labeled after ultraviolet irradiation of excision-defective mutants of Haemophilus influenzae, is of lower single strand molecular weight than that of unirradiated cells but approaches the size of DNA from unirradiated cells upon further incubation in growth medium. This gap-filling ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.176-181.1975
更新日期:1975-10-01 00:00:00
abstract::Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms. The E. coli che region was introduced into Salmonella recipients by means of a series of F-prime e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.107-114.1979
更新日期:1979-07-01 00:00:00
abstract::The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.657-663.1983
更新日期:1983-08-01 00:00:00
abstract::Surgalla, M. J. (Fort Detrick, Frederick, Md.), A. W. Andrews, and C. L. Baugh. Effects of bicarbonate on growth of Pasteurella pestis. I. Differential response of virulent and avirulent cells. J. Bacteriol. 88:269-272. 1964.-Virulent Pasteurella pestis and the avirulent mutants always present in a virulent inoculum h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.2.269-272.1964
更新日期:1964-08-01 00:00:00
abstract::The formation of recombinants in Hfr crosses was studied in Escherichia coli strains carrying combinations of genes known to affect recombination and DNA repair. Mutations in ruv and recG eliminate activities that have been shown to process Holliday junction intermediates by nuclease cleavage and/or branch migration. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1570-1577.1994
更新日期:1994-03-01 00:00:00