Abstract:
:The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted for more than 85% of the released material. Compound 1 (60 to 80% of released material) was a disaccharide tetrapeptide monomer containing a 1,6-anhydromuramic acid residue. Compound 2 (15 to 30% of released material) was a mixture of a tritripeptide and a tritetrapeptide without hexosamines. Taken together the findings suggest that autolytic cell wall degradation in E. coli is selective and involves the activity of both the hydrolytic transglycosylase and an endopeptidase. Upon release, at least some of the wall components were also exposed to the activity of the N-acetylmuramic acid-L-alanine amidase.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kitano K,Tuomanen E,Tomasz Adoi
10.1128/jb.167.3.759-765.1986subject
Has Abstractpub_date
1986-09-01 00:00:00pages
759-65issue
3eissn
0021-9193issn
1098-5530journal_volume
167pub_type
杂志文章abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.169.6.2579-2590.1987
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journal_title:Journal of bacteriology
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abstract::Lysogenization of Salmonella typhimurium with either of the bacteriophages A3 and A4 results in O-acetylation of the L-rhamnose residues of the O-polysaccharide chain of the lipopolysaccharide of the bacterial cell envelope. The O-acetyl group is found on both O-2 and O-3 of the L-rhamnosyl residues. This lysogenic co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1003-1009.1987
更新日期:1987-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.156.2.625-635.1983
更新日期:1983-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.150.2.755-761.1982
更新日期:1982-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1992-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.508-516.1995
更新日期:1995-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2017-08-08 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.646-649.1992
更新日期:1992-01-01 00:00:00
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journal_title:Journal of bacteriology
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abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.999-1005.1971
更新日期:1971-03-01 00:00:00
abstract::The ornithine lipid of Thiobacillus thiooxidans was found to be 1.9% of the total polar lipids. Approximately 80% of this lipid was found to be localized in the outer membrane. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.798-799.1982
更新日期:1982-02-01 00:00:00
abstract::Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2160-2166.1998
更新日期:1998-04-01 00:00:00
abstract::The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.845-857.1980
更新日期:1980-02-01 00:00:00
abstract::The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches. Although tbp2 and tbp3 deletion mutants were readily obtained, a tbp1 mutant was not obtained, and the growth of a conditional tbp1 expre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01165-09
更新日期:2010-03-01 00:00:00