Transglycosylase and endopeptidase participate in the degradation of murein during autolysis of Escherichia coli.

Abstract:

:The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted for more than 85% of the released material. Compound 1 (60 to 80% of released material) was a disaccharide tetrapeptide monomer containing a 1,6-anhydromuramic acid residue. Compound 2 (15 to 30% of released material) was a mixture of a tritripeptide and a tritetrapeptide without hexosamines. Taken together the findings suggest that autolytic cell wall degradation in E. coli is selective and involves the activity of both the hydrolytic transglycosylase and an endopeptidase. Upon release, at least some of the wall components were also exposed to the activity of the N-acetylmuramic acid-L-alanine amidase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kitano K,Tuomanen E,Tomasz A

doi

10.1128/jb.167.3.759-765.1986

subject

Has Abstract

pub_date

1986-09-01 00:00:00

pages

759-65

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

167

pub_type

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