Mapping of rfa Genes in Salmonella typhimurium by ES18 and P22 Transduction and by Conjugation.

Abstract:

:Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthesis of the heptose-containing part of the core. The lipopolysaccharide-indifferent phage ES18 (or its variant ES18.h1) co-transduced rfaF, rfaG, rfaL, rfa(R-res-1), and rfa(R-res-2) alleles with cysE and with pyrE, at rates indicating the order cysE-rfaF-(rfa[R-res-1], rfa[r-res-2], rfaL)-rfaG-pyrE. One proven (and two suspected) rfaE alleles and five proven rfaH alleles were not co-transduced with cysE or pyrE. Hfr crosses indicated that the proven rfaE allele mapped between serA and strA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kuo TT,Stocker BA

doi

10.1128/JB.112.1.48-57.1972

keywords:

subject

Has Abstract

pub_date

1972-10-01 00:00:00

pages

48-57

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

112

pub_type

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