Abstract:
:A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized transport proteins ModA for molybdate and TupA for tungstate. Its structural gene is present in the genome of numerous archaea and some bacteria. The identification of this new tungstate and molybdate binding protein clarifies the mechanism of tungstate and molybdate transport in organisms that lack the known uptake systems associated with the ModA and TupA proteins, like many archaea. The periplasmic protein of this ABC transporter, WtpA (PF0080), was cloned and expressed in Escherichia coli. Using isothermal titration calorimetry, WtpA was observed to bind tungstate (dissociation constant [K(D)] of 17 +/- 7 pM) and molybdate (K(D) of 11 +/- 5 nM) with a stoichiometry of 1.0 mol oxoanion per mole of protein. These low K(D) values indicate that WtpA has a higher affinity for tungstate than do ModA and TupA and an affinity for molybdate similar to that of ModA. A displacement titration of molybdate-saturated WtpA with tungstate showed that the tungstate effectively replaced the molybdate in the binding site of the protein.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bevers LE,Hagedoorn PL,Krijger GC,Hagen WRdoi
10.1128/JB.00548-06subject
Has Abstractpub_date
2006-09-01 00:00:00pages
6498-505issue
18eissn
0021-9193issn
1098-5530pii
188/18/6498journal_volume
188pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2042-2048.1989
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pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2020-07-09 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1115-1123.1975
更新日期:1975-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.826-830.1971
更新日期:1971-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6547-6551.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1469-1475.1968
更新日期:1968-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-08-01 00:00:00
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更新日期:1973-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1995-02-01 00:00:00
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更新日期:1988-09-01 00:00:00
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更新日期:1994-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1995-04-01 00:00:00
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更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.89.5.1370-1373.1965
更新日期:1965-05-01 00:00:00