Intracellular serine protease 1 of Bacillus subtilis is formed in vivo as an unprocessed, active protease in stationary cells.

Abstract:

:Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the protease was found.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sheehan SM,Switzer RL

doi

10.1128/jb.172.1.473-476.1990

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

473-6

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

杂志文章
  • Hybrid Escherichia coli sensory transducers with altered stimulus detection and signaling properties.

    abstract::The tar and tap loci of Escherichia coli encode methyl-accepting inner membrane proteins that mediate chemotactic responses to aspartate and maltose or to dipeptides. These genes lie adjacent to each other in the same orientation on the chromosome and have extensive sequence homology throughout the C-terminal portions...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.2938-2944.1987

    authors: Slocum MK,Halden NF,Parkinson JS

    更新日期:1987-07-01 00:00:00

  • Activation of the σE-dependent stress pathway by conjugative TraR may anticipate conjugational stress.

    abstract::Horizontal gene transfer by conjugation plays a major role in bacterial evolution, allowing the acquisition of new traits, such as virulence and resistance to antibacterial agents. With the increased antibiotic resistance in bacterial pathogens, a better understanding of how bacteria modulate conjugation under changin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02279-14

    authors: Grace ED,Gopalkrishnan S,Girard ME,Blankschien MD,Ross W,Gourse RL,Herman C

    更新日期:2015-03-01 00:00:00

  • Staphylococcus aureus Preferentially Liberates Inorganic Phosphate from Organophosphates in Environments where This Nutrient Is Limiting.

    abstract::Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00264-20

    authors: Kelliher JL,Leder Macek AJ,Grudzinski KM,Radin JN,Kehl-Fie TE

    更新日期:2020-10-22 00:00:00

  • Further electron microscope characterization of spore appendages of Clostridium bifermentans.

    abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.231-238.1968

    authors: Yolton DP,Pope L,Williams MG,Rode LJ

    更新日期:1968-01-01 00:00:00

  • Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion.

    abstract::Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.284-296.1962

    authors: ELLNER PD,BOHAN CD

    更新日期:1962-02-01 00:00:00

  • Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase.

    abstract::The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.6112-6121.1990

    authors: Schlindwein C,Giordano G,Santini CL,Mandrand MA

    更新日期:1990-10-01 00:00:00

  • Candida albicans secreted aspartyl proteinases: isoenzyme pattern is determined by cell type, and levels are determined by environmental factors.

    abstract::For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.18.5215-5221.1995

    authors: White TC,Agabian N

    更新日期:1995-09-01 00:00:00

  • Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.

    abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.816-822.1979

    authors: Brandriss MC

    更新日期:1979-06-01 00:00:00

  • The glucose kinase gene of Streptomyces coelicolor is not required for glucose repression of the chi63 promoter.

    abstract::Glucose kinase is required for glucose repression of several catabolite-controlled genes in Streptomyces coelicolor and certainly plays an important role in glucose repression in these organisms. We report here that glucose kinase null mutants of S. coelicolor retain transcriptional regulation of the chitinase 63 gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.12.3587-3588.1995

    authors: Ingram C,Westpheling J

    更新日期:1995-06-01 00:00:00

  • Transcriptome analysis of sorbic acid-stressed Bacillus subtilis reveals a nutrient limitation response and indicates plasma membrane remodeling.

    abstract::The weak organic acid sorbic acid is a commonly used food preservative, as it inhibits the growth of bacteria, yeasts, and molds. We have used genome-wide transcriptional profiling of Bacillus subtilis cells during mild sorbic acid stress to reveal the growth-inhibitory activity of this preservative and to identify po...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01516-07

    authors: Ter Beek A,Keijser BJ,Boorsma A,Zakrzewska A,Orij R,Smits GJ,Brul S

    更新日期:2008-03-01 00:00:00

  • Light-induced, carrier-mediated transport of tetracycline by Rhodopseudomonas sphaeroides.

    abstract::Tetracycline accumulation by the phototrophic bacterium Rhodopseudomonas sphaeroides has been studied, using the fluorescence properties of the antibiotic and measuring uptake of [7- 3H]tetracycline. Accumulation was carrier mediated, with a Km of approximately 300 micronM. Efflux also appeared to be carried mediated,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.678-683.1979

    authors: Weckesser J,Magnuson JA

    更新日期:1979-06-01 00:00:00

  • Group Q streptococci. II. Nutritional characteristics and growth relationship to thymine, folate, and folinate.

    abstract::The vitamin requirements of the group Q streptococci (Streptococcus avium) and the established enterococcal species (S. faecalis and S. faecium) paralleled one another, although S. avium did not characteristically require riboflavine or pyridoxal. S. avium was further differentiated in that it required thymine for gro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.2.297-299.1967

    authors: Nowlan SS,Deibel RH

    更新日期:1967-08-01 00:00:00

  • Structures containing polyphosphate in Micrococcus lysodeikticus.

    abstract::Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.2.544-553.1968

    authors: Friedberg I,Avigad G

    更新日期:1968-08-01 00:00:00

  • Genome sequence of Mycoplasma iowae strain 695, an unusual pathogen causing deaths in turkeys.

    abstract::Mycoplasma iowae is associated mainly with reduced hatchability in turkeys and is well known for the unusual ability of phenotypic variation in the Mycoplasma surface components as well as a relative resistance to heat, bile salts, and many antimicrobials. A subset of unique genes and a gene cluster responsible for th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06297-11

    authors: Wei S,Guo Z,Li T,Zhang T,Li X,Zhou Z,Li Z,Liu M,Luo R,Bi D,Chen H,Zhou R,Jin H

    更新日期:2012-01-01 00:00:00

  • Genome sequence of the bacteriocin-producing strain Lactococcus garvieae DCC43.

    abstract::This work describes the draft genome sequence of Lactococcus garvieae DCC43. The 2.2-Mb draft genome contains 2,227 predicted protein-coding genes, among which is a region encoding the bacteriocin garvicin ML. No antibiotic resistance genes or capsule-related virulence genes were identified. Two plasmid replication re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01864-12

    authors: Gabrielsen C,Brede DA,Hernández PE,Nes IF,Diep DB

    更新日期:2012-12-01 00:00:00

  • Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.

    abstract::A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.16.4794-4800.1996

    authors: Morbidoni HR,de Mendoza D,Cronan JE Jr

    更新日期:1996-08-01 00:00:00

  • Mutants of Salmonella typhimurium that are insensitive to catabolite repression of proline degradation.

    abstract::In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.2.375-382.1972

    authors: Newell SL,Brill WJ

    更新日期:1972-08-01 00:00:00

  • Oxidation of phenolate siderophores by the multicopper oxidase encoded by the Escherichia coli yacK gene.

    abstract::A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.16.4866-4875.2001

    authors: Kim C,Lorenz WW,Hoopes JT,Dean JF

    更新日期:2001-08-01 00:00:00

  • Oxidation of aliphatic olefins by toluene dioxygenase: enzyme rates and product identification.

    abstract::Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.3858-3865.1997

    authors: Lange CC,Wackett LP

    更新日期:1997-06-01 00:00:00

  • Diguanylate cyclases control magnesium-dependent motility of Vibrio fischeri.

    abstract::Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could mi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00728-06

    authors: O'Shea TM,Klein AH,Geszvain K,Wolfe AJ,Visick KL

    更新日期:2006-12-01 00:00:00

  • Characteristics of some multiply recombination-deficient strains of Escherichia coli.

    abstract::Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13. All multiple rec(-) strains carrying recA13 were similar to AB...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.231-239.1969

    authors: Willetts NS,Clark AJ

    更新日期:1969-10-01 00:00:00

  • Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.

    abstract::The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.18.5971-5977.1992

    authors: Talarico TL,Ray PH,Dev IK,Merrill BM,Dallas WS

    更新日期:1992-09-01 00:00:00

  • Growth pattern of Rickettsia tsutsugamushi in irradiated L cells.

    abstract::Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.879-892.1983

    authors: Hase T

    更新日期:1983-05-01 00:00:00

  • The bile response repressor BreR regulates expression of the Vibrio cholerae breAB efflux system operon.

    abstract::Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00584-08

    authors: Cerda-Maira FA,Ringelberg CS,Taylor RK

    更新日期:2008-11-01 00:00:00

  • Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli.

    abstract::The nucleotide sequence of a 2220-base-pair fragment containing the btuB gene of Escherichia coli was determined. There was a single open reading frame which was translated into a 614-amino-acid polypeptide, the first 20 amino acids of which comprised a typical leader sequence. The putative mature or processed form ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.3.904-908.1985

    authors: Heller K,Kadner RJ

    更新日期:1985-03-01 00:00:00

  • Analysis of storage lipid accumulation in Alcanivorax borkumensis: Evidence for alternative triacylglycerol biosynthesis routes in bacteria.

    abstract::Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01292-06

    authors: Kalscheuer R,Stöveken T,Malkus U,Reichelt R,Golyshin PN,Sabirova JS,Ferrer M,Timmis KN,Steinbüchel A

    更新日期:2007-02-01 00:00:00

  • DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES WITH 8-AZAGUANINE.

    abstract::Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.6.1618-1623.1964

    authors: JOHNSON RC,ROGERS P

    更新日期:1964-12-01 00:00:00

  • Heterotrophic bacteria from cultures of autotrophic Thiobacillus ferrooxidans: relationships as studied by means of deoxyribonucleic acid homology.

    abstract::From several presumably pure cultures of Thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. One was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.448-454.1980

    authors: Harrison AP Jr,Jarvis BW,Johnson JL

    更新日期:1980-07-01 00:00:00

  • A DNA region recognized by the nitric oxide-responsive transcriptional activator NorR is conserved in beta- and gamma-proteobacteria.

    abstract::The sigma(54)-dependent regulator NorR activates transcription of target genes in response to nitric oxide (NO) or NO-generating agents. In Ralstonia eutropha H16, NorR activates transcription of the dicistronic norAB operon that encodes NorA, a protein of unknown function, and NorB, a nitric oxide reductase. A consti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.23.7980-7987.2004

    authors: Büsch A,Pohlmann A,Friedrich B,Cramm R

    更新日期:2004-12-01 00:00:00

  • Characterization of EvgAS-YdeO-GadE branched regulatory circuit governing glutamate-dependent acid resistance in Escherichia coli.

    abstract::Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.21.7378-7389.2004

    authors: Ma Z,Masuda N,Foster JW

    更新日期:2004-11-01 00:00:00