Abstract:
:Horizontal gene transfer by conjugation plays a major role in bacterial evolution, allowing the acquisition of new traits, such as virulence and resistance to antibacterial agents. With the increased antibiotic resistance in bacterial pathogens, a better understanding of how bacteria modulate conjugation under changing environments and the genetic factors involved is needed. Despite the evolutionary advantages conjugation may confer, the process can be quite stressful for the donor cell. Here, we characterize the ability of TraR, encoded on the episomal F' plasmid, to upregulate the σ(E) extracytoplasmic stress pathway in Escherichia coli. TraR, a DksA homolog, modulates transcription initiation through the secondary channel of RNA polymerase. We show here that TraR activates transcription directly; however, unlike DksA, it does so without using ppGpp as a cofactor. TraR expression can stimulate the σ(E) extracytoplasmic stress response independently of the DegS/RseA signal transduction cascade. In the absence of TraR, bacteria carrying conjugative plasmids become more susceptible to external stress. We propose that TraR increases the concentrations of periplasmic chaperones and proteases by directly activating the transcription of σ(E)-dependent promoters; this increased protein folding capacity may prepare the bacterium to endure the periplasmic stress of sex pilus biosynthesis during mating.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Grace ED,Gopalkrishnan S,Girard ME,Blankschien MD,Ross W,Gourse RL,Herman Cdoi
10.1128/JB.02279-14subject
Has Abstractpub_date
2015-03-01 00:00:00pages
924-31issue
5eissn
0021-9193issn
1098-5530pii
JB.02279-14journal_volume
197pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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更新日期:2002-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1970-03-01 00:00:00
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