Abstract:
:In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was tightly linked to the leu operon (at 1.5 min), not to tyrB (at 92 min). Chromosomal fragments cloned because they complemented this mutation were found to complement leu mutations, and vice versa, but none of these fragments complemented a tyrB mutation. The Tn5 insertion and flanking host DNA from this anomalous mutant was cloned in vivo, using Mu dII4042, and an in vivo procedure was developed to isolate deletion derivatives of Tn5-containing plasmids. These deletion plasmids were used to determine the DNA sequences flanking the transposon. The data showed that Tn5 was inserted between bp 122 and 132 in the leu leader. In addition, other ilvE leu double mutants were found to be unable to grow on 2-KIC in place of leucine. The accumulation of 2-ketoisovalerate in ilvE leu double mutants was shown to interfere with 2-KIC amination by the tyrB-encoded transaminase and also by the aspC- and avtA-encoded transaminases (which are able to catalyze this reaction in vivo when the corresponding genes are present on multicopy plasmids).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Vartak NB,Liu L,Wang BM,Berg CMdoi
10.1128/jb.173.12.3864-3871.1991keywords:
subject
Has Abstractpub_date
1991-06-01 00:00:00pages
3864-71issue
12eissn
0021-9193issn
1098-5530journal_volume
173pub_type
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abstract::Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...
journal_title:Journal of bacteriology
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doi:10.1128/JB.91.3.953-962.1966
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journal_title:Journal of bacteriology
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doi:10.1128/JB.88.1.151-157.1964
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abstract::The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined. The pspF gene encodes a sigma54 transcriptional activator of the phage shock protein (psp) operon (pspA to pspE). Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL and goaG, convergently oriente...
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.442-445.1979
更新日期:1979-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1085-1097.1966
更新日期:1966-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2433-2438.1990
更新日期:1990-05-01 00:00:00
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doi:10.1128/JB.95.1.132-138.1968
更新日期:1968-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.464-471.1985
更新日期:1985-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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更新日期:1964-02-01 00:00:00
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更新日期:1995-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.4031-4037.1989
更新日期:1989-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.135.2.528-541.1978
更新日期:1978-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-06-01 00:00:00
abstract:UNLABELLED:Edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. However, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. Here, we show that E. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine ma...
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pub_type: 杂志文章
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更新日期:2016-04-28 00:00:00