Abstract:
:Rohlfing, S. R. (Western Reserve University, Cleveland, Ohio), and I. P. Crawford. Purification and characterization of the beta-galactosidase of Aeromonas formicans. J. Bacteriol. 91:1085-1097. 1966.-The beta-galactosidase of Aeromonas formicans was purified by diethylaminoethyl cellulose chromatography and gel filtration on Sephadex G-200. The properties of the enzyme molecule were compared with purified beta-galactosidase from Escherichia coli. The sedimentation coefficients and electrophoretic mobilities of the two enzymes were not significantly different; the electrophoretic mobility of urea-produced subunits of the two enzymes was also similar. The stabilities of the two enzymes to denaturing agents provided measurable differences; E. coli beta-galactosidase is relatively more heat-stable and more resistant to the action of urea. The amino acid compositions of the two proteins revealed significant differences in several amino acids, particularly alanine, arginine, glycine, and leucine. The comparisons cited suggest that A. formicans and E. coli are not completely unrelated, for their beta-galactosidases show considerable structural similarity.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rohlfing SR,Crawford IPdoi
10.1128/JB.91.3.1085-1097.1966subject
Has Abstractpub_date
1966-03-01 00:00:00pages
1085-97issue
3eissn
0021-9193issn
1098-5530journal_volume
91pub_type
杂志文章abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1776-1782.2003
更新日期:2003-03-01 00:00:00
abstract::The HPB12 protein from the nucleoid of Bacillus subtilis was previously described, and its DNA binding properties have been reported previously (V. Salti, F. Le Hégarat, and L. Hirschbein, Biochim. Biophys. Acta 1009:161-167, 1989). The DNA-HPB12 complexes were examined by electron microscopy. They appeared as short, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.1.50-60.1994
更新日期:1994-01-01 00:00:00
abstract::The growth of Bacillus subtilis mutant betaA177 can be inhibited under special conditions in which not enough autolytic enzymes are produced for optimal growth. Electron microscopy studies show that during growth inhibition there is localized thickening of the cell wall at positions where cells bend. A model is propos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.3.1247-1257.1972
更新日期:1972-03-01 00:00:00
abstract::Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01578-08
更新日期:2009-04-01 00:00:00
abstract::The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5084-5089.2005
更新日期:2005-08-01 00:00:00
abstract::Spontaneous mutators of Salmonella typhimurium LT2 were generated by inserting the transposable element Tn5 or Tn10 into the bacterial chromosome. Two mutators mapped at the position of the mutH and mutL loci of S. typhimurium, and two other mutators mapped at positions corresponding to the mutS and uvrD loci of Esche...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.827-835.1981
更新日期:1981-09-01 00:00:00
abstract::Rabbits immunized with mucopeptide derived from cell wall debris of Streptococcus agalactiae by use of the formamide extraction technique developed specific antibodies for bovine heart, skeletal muscle, lymph nodes, and blood buffy coat extracts. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.577-579.1967
更新日期:1967-02-01 00:00:00
abstract::Slein, Milton W. (Fort Detrick, Frederick, Md.) and Gerald F. Logan, Jr. Mechanism of action of the toxin of Bacillus anthracis. II. Alkaline phosphatasemia produced by culture filtrates of various bacilli. J. Bacteriol. 83:359-369. 1962.-A factor which produces hyperphosphatasemia after intravenous injection into ani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.359-369.1962
更新日期:1962-02-01 00:00:00
abstract::A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.862-870.1998
更新日期:1998-02-01 00:00:00
abstract::We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.9.2711-2722.2003
更新日期:2003-05-01 00:00:00
abstract::We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4665-4684.2004
更新日期:2004-07-01 00:00:00
abstract::Mutations in a regulator gene (capR) that causes increased synthesis of capsular polysaccharide and derepressed synthesis of several enzymes involved in polysaccharide synthesis also derepress synthesis of guanosine diphosphate (GDP)-mannose pyrophosphorylase. In addition, a second mucoid mutation (capS, which maps se...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.965-972.1970
更新日期:1970-03-01 00:00:00
abstract::Haemophilus parasuis is a swine pathogen of significant industry concern, but little is known about how the organism causes disease. A related human pathogen, Haemophilus influenzae, has been better studied, and many of its virulence factors have been identified. Two of these, outer membrane proteins P2 and P5, are kn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00469-09
更新日期:2009-10-01 00:00:00
abstract::Oxygen utilization in splenic cells of normal and BCG-sensitized guinea pigs was measured in a Warburg respirometer in the absence and in the presence of Old Tuberculin (OT). In the absence of OT, normal and sensitive cells respired at the same rate; in the presence of OT, normal cell respiration remained unchanged, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.1-5.1969
更新日期:1969-01-01 00:00:00
abstract::The spores of Bacillus subtilis show remarkable resistance to many environmental stresses, due in part to the presence of an outer proteinaceous structure known as the spore coat. GerQ is a spore coat protein essential for the presence of CwlJ, an enzyme involved in the hydrolysis of the cortex during spore germinatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5567-5575.2004
更新日期:2004-09-01 00:00:00
abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01569-07
更新日期:2008-10-01 00:00:00
abstract::Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.1703-1710.1990
更新日期:1990-04-01 00:00:00
abstract::A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.769-771.1985
更新日期:1985-02-01 00:00:00
abstract::Shah, D. B. (University of Wisconsin, Madison), and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. II. Characterization of the lipase activity. J. Bacteriol. 89:949-953. 1965.-The staphylococcal egg yolk factor was characterized as a lipase. The enzyme had an optimal pH of 7.8, but the optimal pH of stability...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.949-953.1965
更新日期:1965-04-01 00:00:00
abstract::rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.20.6185-6191.2003
更新日期:2003-10-01 00:00:00
abstract::We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.4719-4729.2000
更新日期:2000-09-01 00:00:00
abstract::Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2318-2321.1987
更新日期:1987-05-01 00:00:00
abstract:UNLABELLED:Rhodobacter sphaeroides is a free-living alphaproteobacterium that contains two clusters of functional flagellar genes in its genome: one acquired by horizontal gene transfer (fla1) and one that is endogenous (fla2). We have shown that the Fla2 system is normally quiescent and under certain conditions produc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00170-15
更新日期:2015-09-01 00:00:00
abstract::Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5741-5747.1996
更新日期:1996-10-01 00:00:00
abstract::A previously undetected Bacillus subtilis aspartokinase isozyme, which we have called aspartokinase III, has been characterized. The new isozyme was most readily detected in extracts of cells grown with lysine, which repressed aspartokinase II and induced aspartokinase III, or in extracts of strain VS11, a mutant lack...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.218-223.1990
更新日期:1990-01-01 00:00:00
abstract::Campbell, J. J. R. (The University of British Columbia, Vancouver, B.C., Canada), Loretta A. Hogg, and G. A. Strasdine. Enzyme distribution in Pseudomonas aeruginosa. J. Bacteriol. 83:1155-1160. 1962.-Previous studies on the distribution of enzymes in bacteria have indicated that, although individual enzymes were pred...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.5.1155-1160.1962
更新日期:1962-05-01 00:00:00
abstract:UNLABELLED:Bacteriophytochrome photoreceptors (BphPs) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. Most of these systems utilize single-domain response regulators to transduce signal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00872-15
更新日期:2016-03-31 00:00:00
abstract::Microtubular structures, which were demonstrated as straight, dense-walled cylinders attached to cell membranes, were found in a stable staphylococcal L-form grown in the absence of antibiotic. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.3.1024-1026.1977
更新日期:1977-12-01 00:00:00
abstract::A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.13.3673-3679.1995
更新日期:1995-07-01 00:00:00
abstract::Capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (W. Godchaux III and E. R. Leadbetter, J. Bacteriol. 144:592-602, 1980). One of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, N-acylated versions of capnine. The lipids were foun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1238-1246.1983
更新日期:1983-03-01 00:00:00