Abstract:
:rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificially changing ribosome synthesis rates and observing responses of rRNA promoters. However, it has not been demonstrated previously that the initiating nucleoside triphosphate (iNTP) and guanosine 5'-diphosphate 3'-diphosphate (ppGpp), the molecular effectors responsible for controlling rRNA promoters in response to changes in the nutritional environment, are responsible for altering rRNA promoter activities under these feedback conditions. Here, we show that most feedback situations result in changes in the concentrations of both the iNTP and ppGpp and that the directions of these changes are consistent with a role for these two small-molecule regulators in feedback control of rRNA synthesis. In contrast, we observed no change in the level of DNA supercoiling under the feedback conditions examined.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schneider DA,Gourse RLdoi
10.1128/jb.185.20.6185-6191.2003keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
6185-91issue
20eissn
0021-9193issn
1098-5530journal_volume
185pub_type
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journal_title:Journal of bacteriology
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更新日期:1988-04-01 00:00:00
abstract::A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates. Genes encoding the enzymes that catabolize intracellular glucose to pyruvate and glyceraldehyde 3-phosphate are coordinately regulated, clustered at 39 min on the chromosome, and collectively form the hex re...
journal_title:Journal of bacteriology
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更新日期:2011-01-01 00:00:00
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doi:10.1128/JB.149.1.106-113.1982
更新日期:1982-01-01 00:00:00
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doi:10.1128/JB.181.14.4154-4160.1999
更新日期:1999-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-04-01 00:00:00
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更新日期:1966-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.159.1.57-62.1984
更新日期:1984-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1964-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1983-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.6.1270-1274.1963
更新日期:1963-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6041-6047.1997
更新日期:1997-10-01 00:00:00
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更新日期:1979-09-01 00:00:00
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更新日期:2003-04-01 00:00:00
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更新日期:1996-07-01 00:00:00
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更新日期:1987-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1991-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
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更新日期:2001-03-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1985-04-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2012-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1991-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00