Abstract:
:A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both pathogenicity toward tomato and ability to induce hypersensitive reaction (HR) on tobacco (hrp mutants). The insert is colinear with the genome and provides restoration of the HR-inducing ability when transferred into several Tn5-induced hrp mutants, but failed to complement deletion mutants extending on both sides of the pVir2 region. Localized mutagenesis demonstrated that the hrp genes are clustered within a 17.5-kb region of pVir2 and that this cluster probably extends on the genomic region adjacent to the pVir2 insert. A 3-kb region adjacent to the hrp cluster modulates aggressiveness toward tomato but does not control HR-inducing ability. Sequences within the hrp cluster of pVir2 have homology with the genomic DNA of Xanthomonas campestris strains representing eight different pathovars, suggesting that a set of common pathogenicity functions could be shared by P. solanacearum and X. campestris.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Boucher CA,Van Gijsegem F,Barberis PA,Arlat M,Zischek Cdoi
10.1128/jb.169.12.5626-5632.1987subject
Has Abstractpub_date
1987-12-01 00:00:00pages
5626-32issue
12eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.9.3273-3279.2006
更新日期:2006-05-01 00:00:00
abstract::In Escherichia coli, the additon of ethanol resulted in the synthesis of an increased proportion of phospholipids containing two unsaturated fatty acids. The addition of hexanol resulted in the opposite effect, an increase in the proportion of monounsaturated molecular species. The alcohol-induced changes were quantit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.3.1040-1044.1980
更新日期:1980-06-01 00:00:00
abstract::Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535-545. 1966.-Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.535-545.1966
更新日期:1966-02-01 00:00:00
abstract::In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.254-261.1980
更新日期:1980-04-01 00:00:00
abstract::The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.327-330.1992
更新日期:1992-01-01 00:00:00
abstract::The genes for the complete pathways for two polycyclic aromatic polyketides of the angucyclinone class have been cloned and heterologously expressed. Genomic DNAs of Streptomyces rimosus NRRL 3016 and Streptomyces strain WP 4669 were partially digested with MboI, and libraries (ca. 40-kb fragments) in Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.470-476.1997
更新日期:1997-01-01 00:00:00
abstract::Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat inno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.1-7.1974
更新日期:1974-01-01 00:00:00
abstract::Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00607-10
更新日期:2010-10-01 00:00:00
abstract::Copper (Cu) is a required micronutrient, but it is highly toxic at high concentrations. Therefore, the levels of Cu must be tightly regulated in all living cells. The phagosome of Mycobacterium tuberculosis has been shown to have variable levels of Cu. Previously, we showed that M. tuberculosis contains a copper-sensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01847-07
更新日期:2008-04-01 00:00:00
abstract::We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.7.2711-2714.2006
更新日期:2006-04-01 00:00:00
abstract::Dworkin, Martin (University of Minnesota, Minneapolis), and Donald J. Niederpruem. Electron transport system in vegetative cells and microcysts of Myxococcus xanthus. J. Bacteriol. 87:316-322. 1964.-Respiration by intact cells of the fruiting myxobacterium Myxococcus xanthus is cyanide-sensitive and can be demonstrate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.316-322.1964
更新日期:1964-02-01 00:00:00
abstract::Structural analogs of S-adenosylhomocysteine were tested in vitro for inhibition of the yeast S-adenosylmethionine:delta 24-sterol-C-methyltransferase enzyme. A wide inhibitory range by these compounds was observed, suggesting which structural features of the parent compound are important for binding to the enzyme. No...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.106-112.1981
更新日期:1981-01-01 00:00:00
abstract::Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00477-09
更新日期:2009-09-01 00:00:00
abstract::The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.20.5625-5632.2002
更新日期:2002-10-01 00:00:00
abstract::The Methanococcus maripaludis energy-conserving hydrogenase B (Ehb) generates low potential electrons required for autotrophic CO(2) assimilation. To analyze the importance of individual subunits in Ehb structure and function, markerless in-frame deletions were constructed in a number of M. maripaludis ehb genes. Thes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01446-09
更新日期:2010-08-01 00:00:00
abstract::A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.495-501.1974
更新日期:1974-10-01 00:00:00
abstract::The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00330-07
更新日期:2007-07-01 00:00:00
abstract::Genes involved in the biosynthesis of leucine have been mapped in Bacillus megaterium QM B1551, using transducing phage MP13. Mutations were designated leuA, leuB, or leuC on the basis of enzyme assays. Two mutant strains were deficient in the enzyme activities of leuA (alpha-isopropylmalate synthase) and leuC (beta-i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.454-459.1984
更新日期:1984-02-01 00:00:00
abstract::Sigma 54 associates with bacterial core RNA polymerase and converts it into an enhancer-responsive enzyme. Deletion of the N-terminal 40 amino acids is known to result in loss of the ability to respond to enhancer binding proteins. In this work PCR mutagenesis and genetic screens were used to identify a small patch, f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5619-5625.1998
更新日期:1998-11-01 00:00:00
abstract::Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01215-12
更新日期:2012-11-01 00:00:00
abstract::The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00577-10
更新日期:2010-09-01 00:00:00
abstract::Uracil-DNA glycosylase activity was found in Streptococcus pneumoniae, and the enzyme was partially purified. An ung mutant lacking the activity was obtained by positive selection of cells transformed with a plasmid containing uracil in its DNA. The effects of the ung mutation on mutagenic processes in S. pneumoniae w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.283-290.1991
更新日期:1991-01-01 00:00:00
abstract::Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02136-12
更新日期:2013-03-01 00:00:00
abstract::Escherichia coli sigma54-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator. Sigma70-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF deltaHTH. Negative autoregulation of PspF...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.16.5232-5237.1997
更新日期:1997-08-01 00:00:00
abstract::The entry of oligogalacturonates into Dickeya dadantii occurs through the specific channel KdgM. The genome of the bacterium encodes a second member of this family of outer membrane proteins, KdgN. We showed that this protein is also involved in the uptake of oligogalacturonates. When KdgN was reconstituted in proteol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00218-07
更新日期:2007-08-01 00:00:00
abstract::The expression of Escherichia coli purR, which encodes the pur regulon repressor protein, is autoregulated. Autoregulation at the level of transcription requires two operator sites, designated purRo1 and purRo2 (O1 and O2). Operator O1 is in the region of DNA between the transcription start site and the site for trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5758-5766.1990
更新日期:1990-10-01 00:00:00
abstract::In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00401-10
更新日期:2010-10-01 00:00:00
abstract::Mycelial mats of Ascodesmis sphaerospora were fixed and embedded for electron microscopy, and thin sections of 1-mm blocks, taken from the 1st to the 7th mm behind the hyphal tips, were cut parallel to the long axis of the hyphae. The hyphal tip region is characterized by an outer zone of electron-transparent vesicles...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.658-671.1968
更新日期:1968-02-01 00:00:00
abstract::The importance of proton movements was assessed in the moderate halophile Vibrio costicola. When anaerobic cells in acidic buffer (pH 6.5) were given an O2 pulse, protons were extruded regardless of the presence of Na+. At pH 8.5, however, V. costicola produced an acidic response to an O2 pulse in the absence of Na+ a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.681-686.1985
更新日期:1985-02-01 00:00:00
abstract::Vibrio cholerae uses a variety of strategies for obtaining iron in its diverse environments. In this study we report the identification of a novel iron utilization protein in V. cholerae, VciB. The vciB gene and its linked gene, vciA, were isolated in a screen for V. cholerae genes that permitted growth of an Escheric...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00569-08
更新日期:2008-09-01 00:00:00