EFFECTS OF BICARBONATE ON GROWTH OF PASTEURELLA PESTIS. I. DIFFERENTIAL RESPONSE OF VIRULENT AND AVIRULENT CELLS.

abstract：:Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

journal_title：Journal of bacteriology

authors： Bingle WH,Nomellini JF,Smit J

更新日期：1997-02-01 00:00:00

abstract：:The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

journal_title：Journal of bacteriology

authors： Cosby WM,Zuber P

更新日期：1997-11-01 00:00:00

abstract：:Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A ...

journal_title：Journal of bacteriology

authors： Marcus SL,Reeves HC,Ajl SJ

更新日期：1968-10-01 00:00:00

abstract：:Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

journal_title：Journal of bacteriology

authors： Metcalf WW,Steed PM,Wanner BL

更新日期：1990-06-01 00:00:00

abstract：:Piliated, competent gonococci are known to preferentially take up homologous transforming DNA into the cell. We examined the mechanism for DNA uptake with pFA10, a hybrid 11.5-kilobase (kb) penicillin-resistant (Pcr) plasmid composed of heterologous DNA from a 7.2-kb Pcr plasmid and homologous DNA from a 4.2-kb gonoco...

journal_title：Journal of bacteriology

authors： Graves JF,Biswas GD,Sparling PF

更新日期：1982-12-01 00:00:00

abstract：:Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose w...

journal_title：Journal of bacteriology

authors： Bhumiratana A,Anderson RL,Costilow RN

更新日期：1974-08-01 00:00:00

abstract：:DNA involved in the mobilization of broad-host-range plasmid R1162 was localized to a region of 2.7 kilobases within coordinates 3.4 to 6.1 kilobases on the R1162 map. By examining the transfer properties of plasmids containing cloned fragments of DNA from within this region, we showed that at least four trans-active ...

journal_title：Journal of bacteriology

authors： Brasch MA,Meyer RJ

更新日期：1986-08-01 00:00:00

abstract：:Escherichia coli UM146 was isolated from the ileum of a Crohn's disease patient. It adheres to and invades enterocytes and can replicate inside macrophages. Its complete genome sequence reveals that it is most closely related to the human urinary tract pathogen E. coli CFT073, but it has a host of genes that are novel...

journal_title：Journal of bacteriology

authors： Krause DO,Little AC,Dowd SE,Bernstein CN

更新日期：2011-01-01 00:00:00

abstract：:A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

journal_title：Journal of bacteriology

authors： Baum JA

更新日期：1994-05-01 00:00:00

abstract：:Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia...

journal_title：Journal of bacteriology

authors： Dreyfus LA,Iglewski BH

更新日期：1985-01-01 00:00:00

abstract：:Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...

journal_title：Journal of bacteriology

authors： Pucci MJ,Daneo-Moore L

更新日期：1984-08-01 00:00:00

abstract：:A genetic system was developed to investigate the supercoil structure of bacterial chromosomes. New res-carrying transposons were derived from MudI1734 (MudJr1 and MudJr2) and Tn10 (Tn10dGn). The MudJr1 and MudJr2 elements each have a res site in opposite orientation so that when paired with a Tn10dGn element in the s...

journal_title：Journal of bacteriology

authors： Higgins NP,Yang X,Fu Q,Roth JR

更新日期：1996-05-01 00:00:00

abstract：:The promoter selectivity of two extracytoplasmic function (ECF) subfamily sigma subunits, sigma(E) (sigma(24)) and sigma(FecI) (sigma(18)), of Escherichia coli RNA polymerase was analyzed by using an in vitro transcription system and various promoters. The Esigma(E) holoenzyme recognized only the known cognate promote...

journal_title：Journal of bacteriology

authors： Maeda H,Jishage M,Nomura T,Fujita N,Ishihama A

更新日期：2000-02-01 00:00:00

abstract：:The molecular basis of the ability of bacteria to live on caffeine via the C-8 oxidation pathway is unknown. The first step of this pathway, caffeine to trimethyluric acid (TMU), has been attributed to poorly characterized caffeine oxidases and a novel quinone-dependent caffeine dehydrogenase. Here, we report the deta...

journal_title：Journal of bacteriology

authors： Mohanty SK,Yu CL,Das S,Louie TM,Gakhar L,Subramanian M

更新日期：2012-08-01 00:00:00

abstract：:The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...

journal_title：Journal of bacteriology

authors： Briles EB,Tomasz A

更新日期：1975-04-01 00:00:00

abstract：:The carbon monoxide (CO) dehydrogenase activity band from Clostridium pasteurianum was shown to contain nickel by in situ activity staining of polyacrylamide gels. However, the majority of the nickel in cell extracts was found to electrophorese independently of CO dehydrogenase. Comparative analysis with Clostridium t...

journal_title：Journal of bacteriology

authors： Drake HL

更新日期：1982-02-01 00:00:00

abstract：:When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a beta-galactosidase gene transcriptionally fused to the inactivated target gene, was used to ide...

journal_title：Journal of bacteriology

authors： Prágai Z,Eschevins C,Bron S,Harwood CR

更新日期：2001-04-01 00:00:00

abstract：:The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...

journal_title：Journal of bacteriology

authors： Chen MW,Kotaka M,Vonrhein C,Bricogne G,Rao F,Chuah ML,Svergun D,Schneider G,Liang ZX,Lescar J

更新日期：2012-09-01 00:00:00

abstract：:Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by means of an oligohistid...

journal_title：Journal of bacteriology

authors： Smith DL,Young R

更新日期：1998-08-01 00:00:00

abstract：:The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is...

journal_title：Journal of bacteriology

authors： Zúñiga-Ripa A,Barbier T,Conde-Álvarez R,Martínez-Gómez E,Palacios-Chaves L,Gil-Ramírez Y,Grilló MJ,Letesson JJ,Iriarte M,Moriyón I

更新日期：2014-08-15 00:00:00

abstract：:CluA is a cell surface-presented protein that causes cell aggregation and is essential for a high-efficiency conjugation process in Lactococcus lactis. We know from previous work that in addition to promoting cell-to-cell contact, CluA is involved in sex factor DNA transfer. To define the CluA domains involved in aggr...

journal_title：Journal of bacteriology

authors： Stentz R,Gasson M,Shearman C

更新日期：2006-03-01 00:00:00

abstract：:We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is positively growth rate regulated in exponentially growing bacteria and...

journal_title：Journal of bacteriology

authors： Tsui HC,Arps PJ,Connolly DM,Winkler ME

更新日期：1991-11-01 00:00:00

abstract：:The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

journal_title：Journal of bacteriology

authors： Duncan CL,Sugiyama H,Strong DH

更新日期：1968-05-01 00:00:00

abstract：:The respiratory control of adhE, which encodes ethanol (alcohol) dehydrogenase in Escherichia coli, was examined at the transcriptional level by using various phi (adhE'-lacZ) adhE+ merodiploid strains. Expression of the adhE'-lacZ operon fusion was increased about eightfold by anaerobic growth. Under anaerobic growth...

journal_title：Journal of bacteriology

authors： Chen YM,Lin EC

更新日期：1991-12-01 00:00:00

abstract：:The pattern of transcription of the rplKAJLrpoBC gene cluster of Escherichia coli appears to be complex. At least four different promoters and a transcriptional attenuator have been identified. To compare the relative effect of each of the putative promoters and the attenuator on transcription of these genes, we fused...

journal_title：Journal of bacteriology

authors： Ralling G,Linn T

更新日期：1984-04-01 00:00:00

abstract：:Pyridine-2,6-bis(thiocarboxylate) (PDTC), produced by certain pseudomonads, is a sulfur-containing siderophore that binds iron, as well as a wide range of transition metals, and it affects the net hydrolysis of the environmental contaminant carbon tetrachloride. The pathway of PDTC biosynthesis has not been defined. H...

journal_title：Journal of bacteriology

authors： Lewis TA,Glassing A,Harper J,Franklin MJ

更新日期：2013-09-01 00:00:00

abstract：:The Escherichia coli beta-galactosidase enzyme was used as a reporter molecule for genetic fusions in Rhodobacter capsulatus. DNA fragments that were from the upstream region of the hydrogenase structural operon hupSLM and contained 5' hupS sequences were fused in frame to a promoterless lacZ gene, yielding fusion pro...

journal_title：Journal of bacteriology

authors： Colbeau A,Vignais PM

更新日期：1992-07-01 00:00:00

abstract：:The citrate utilization (Cit+) transposon Tn3411 was shown to be flanked by directly repeated sequences (IS3411L and IS3411R) by restriction enzyme analysis and electron microscope observation. Cit- deletion mutants were frequently found to be generated in pBR322::Tn3411 by intramolecular recombination between the two...

journal_title：Journal of bacteriology

authors： Ishiguro N,Sato G

更新日期：1984-11-01 00:00:00

abstract：:CatR, a LysR family protein, positively regulates the Pseudomonas putida catBC operon, which is required for growth on benzoate as a sole carbon source. Transcriptional studies show that the catR and catBC promoters are divergent and overlapping by 2 bp. A beta-galactosidase promoter probe vector was constructed to an...

journal_title：Journal of bacteriology

authors： Rothmel RK,Shinabarger DL,Parsek MR,Aldrich TL,Chakrabarty AM

更新日期：1991-08-01 00:00:00

abstract：UNLABELLED:Biofilm formation is responsible for increased antibiotic tolerance in pathogenic bacteria. Cyclic di-GMP (c-di-GMP) is a widely used second-messenger signal that plays a key role in bacterial biofilm formation. c-di-GMP is synthesized by diguanylate cyclases (DGCs), a conserved class of enzymes absent in ma...

journal_title：Journal of bacteriology

authors： Fernicola S,Paiardini A,Giardina G,Rampioni G,Leoni L,Cutruzzolà F,Rinaldo S

更新日期：2015-09-28 00:00:00