Abstract:
:The pattern of transcription of the rplKAJLrpoBC gene cluster of Escherichia coli appears to be complex. At least four different promoters and a transcriptional attenuator have been identified. To compare the relative effect of each of the putative promoters and the attenuator on transcription of these genes, we fused these regulatory sites to lacZ. These transcriptional fusions were constructed on lambda transducing phages so a single copy of each could be stably integrated into the chromosome. The level of beta-galactosidase in a lysogen of each phage reflects the activity of the transcriptional regulatory site. We find that the promoters preceding rplK (rplKp) and rplJ (rplJp) are indeed the major promoters of this gene cluster. The minor promoter before rplL (rplLp) is much weaker and contributes little to the transcription of the downstream genes. Under these conditions, we find no evidence of a promoter (rpoBp) in the rplL-rpoB intercistronic region. The attenuator (atn) terminates ca. 70% of the transcripts initiated at the promoters preceding it. Although we cannot rule out that some transcripts from rplKp may read through into rplJLrpoBC, we find that rplJp alone is sufficient for high-level expression of these genes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ralling G,Linn Tdoi
10.1128/JB.158.1.279-285.1984subject
Has Abstractpub_date
1984-04-01 00:00:00pages
279-85issue
1eissn
0021-9193issn
1098-5530journal_volume
158pub_type
杂志文章abstract::The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.869-874.1981
更新日期:1981-09-01 00:00:00
abstract::Feldman, L. A. (Baylor University College of Medicine, Houston, Tex.), J. L. Melnick, and F. Rapp. Influence of SV40 genome on the replication of an adenovirus-SV40 "hybrid" population. J. Bacteriol. 90:778-782. 1965.-Replication of a type 7 adenovirus-SV40 hybrid population in primary African green monkey kidney cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.778-782.1965
更新日期:1965-09-01 00:00:00
abstract::Bacillus subtilis grows under anaerobic conditions utilizing nitrate ammonification and various fermentative processes. The two-component regulatory system ResDE and the redox regulator Fnr are the currently known parts of the regulatory system for anaerobic adaptation. Mutation of the open reading frame ywiD located ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.23.6815-6821.2001
更新日期:2001-12-01 00:00:00
abstract::Microbacterium barkeri strain 2011-R4 is a Gram-positive epiphyte which has been confirmed as a biocontrol agent against several plant pathogens in our previous studies. Here, we present the draft genome sequence of this strain, which was isolated from the rice rhizosphere in Tonglu city, Zhejiang province, China. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01468-12
更新日期:2012-12-01 00:00:00
abstract::Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1386-1394.1979
更新日期:1979-03-01 00:00:00
abstract::GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated an open reading frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.13.4045-4051.1993
更新日期:1993-07-01 00:00:00
abstract::LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.5.1207-1214.1998
更新日期:1998-03-01 00:00:00
abstract::The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was clon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5661-5671.2004
更新日期:2004-09-01 00:00:00
abstract::Strains monosomic for chromosome I of Saccharomyces cerevisiae contain 25 to 35% fewer rRNA genes than do normal diploid strains. When these strains are repeatedly subcultured, colonies are isolated that have magnified their number of rRNA genes to the diploid amount while remaining monosomic for chromosome I. We have...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.1.237-245.1978
更新日期:1978-04-01 00:00:00
abstract::CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.1037-1044.2003
更新日期:2003-02-01 00:00:00
abstract::Strains of Lactobacillus casei subsp. rhamnosus possessing two cell wall polysaccharides, a hexosamine-containing H-polysaccharide and a rhamnose-containing R-polysaccharide, were examined for the effect of growth conditions on the production of these two components. In strain NCTC 6375, R- and H-polysaccharides accou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.84-92.1983
更新日期:1983-01-01 00:00:00
abstract::sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4629-4637.1992
更新日期:1992-07-01 00:00:00
abstract::With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1228-1233.1986
更新日期:1986-12-01 00:00:00
abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1133-1136.1979
更新日期:1979-12-01 00:00:00
abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.299-302.1989
更新日期:1989-01-01 00:00:00
abstract::Bacterial biofilms are important in natural settings, biotechnology, and medicine. However, regulation of biofilm development and its persistence in different niches is complex and only partially understood. One key step during the biofilm life cycle is dispersal, when motile cells abandon the mature biofilm to spread...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00473-18
更新日期:2018-12-20 00:00:00
abstract::The 2-(methylthio)ethanesulfonic acid (CH3-S-CoM) reductase catalyzes the final methane-yielding reaction in fastidiously anaerobic methanogenic archaebacteria. This step involves the reductive demethylation of CH3-S-CoM with reducing equivalents from N-7-(mercaptoheptanoyl)-L-threonine O3-phosphate (HS-HTP) to yield ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.1007-1012.1992
更新日期:1992-02-01 00:00:00
abstract::Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.1281-1284.1968
更新日期:1968-10-01 00:00:00
abstract::Immunological methods were used to examine the flagellin production of Salmonella typhimurium strains that carried a mutation in one of the two possible genes for flagellin (H1 or H2) and also were incapable of expressing the other gene. Some mutants produced flagellin that was excreted into the culture medium; others...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.1.291-296.1987
更新日期:1987-01-01 00:00:00
abstract::The pink yeast Rhodotorula rubra of marine origin was found to be capable of extended growth at very low phosphate concentrations (K(0.5) = 10.8 nm). Average intracellular phosphate concentrations, based on isotope exchange techniques, were 15 to 200 nm, giving concentration gradients across the cell envelope of about...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.599-611.1973
更新日期:1973-02-01 00:00:00
abstract::Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.7.1900-1902.1995
更新日期:1995-04-01 00:00:00
abstract::rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.20.6185-6191.2003
更新日期:2003-10-01 00:00:00
abstract::The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00406-08
更新日期:2008-07-01 00:00:00
abstract::The enhancer-binding protein NIFA is required for transcriptional activation of nif promoters by the alternative holoenzyme form of RNA polymerase, which contains the sigma factor sigma 54 (sigma N). NIFA hydrolyzes nucleoside triphosphates to catalyze the isomerization of closed promoter complexes to transcriptionall...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.5.1186-1195.1995
更新日期:1995-03-01 00:00:00
abstract::Tyrosine-mediated repression of aroF and tyrP was studied by inserting DNA sequences between the two adjacent TYR R boxes which, in each case, overlap the respective RNA polymerase binding sites of these genes. In both cases, repression was greatest when homologous regions of these two TYR R boxes were on the same fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5079-5085.1991
更新日期:1991-08-01 00:00:00
abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.35-45.1996
更新日期:1996-01-01 00:00:00
abstract::The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1219-1222.1989
更新日期:1989-02-01 00:00:00
abstract::The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::The composition of the Pseudomonas solanacearum lipolysaccharide (LPS) was found to be similar to that described for the LPS of enterobacteria. The lipid A contained fatty acids and glucosamine in a molar ratio of 5:2. The LPS fraction contained 2-keto-3-deoxyoctulosonic acid, L-glycero-D-mannoheptose, hexoses (glucos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.504-509.1985
更新日期:1985-05-01 00:00:00
abstract::The transcriptional regulatory protein Rgg coordinates amino acid catabolism and virulence factor expression in Streptococcus pyogenes. We used a proteomic approach to compare cytoplasmic proteins isolated from S. pyogenes wild-type strain NZ131 (serotype M49) to proteins isolated from an rgg mutant strain during the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7091-7099.2004
更新日期:2004-11-01 00:00:00