Abstract:
:The pattern of transcription of the rplKAJLrpoBC gene cluster of Escherichia coli appears to be complex. At least four different promoters and a transcriptional attenuator have been identified. To compare the relative effect of each of the putative promoters and the attenuator on transcription of these genes, we fused these regulatory sites to lacZ. These transcriptional fusions were constructed on lambda transducing phages so a single copy of each could be stably integrated into the chromosome. The level of beta-galactosidase in a lysogen of each phage reflects the activity of the transcriptional regulatory site. We find that the promoters preceding rplK (rplKp) and rplJ (rplJp) are indeed the major promoters of this gene cluster. The minor promoter before rplL (rplLp) is much weaker and contributes little to the transcription of the downstream genes. Under these conditions, we find no evidence of a promoter (rpoBp) in the rplL-rpoB intercistronic region. The attenuator (atn) terminates ca. 70% of the transcripts initiated at the promoters preceding it. Although we cannot rule out that some transcripts from rplKp may read through into rplJLrpoBC, we find that rplJp alone is sufficient for high-level expression of these genes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ralling G,Linn Tdoi
10.1128/JB.158.1.279-285.1984subject
Has Abstractpub_date
1984-04-01 00:00:00pages
279-85issue
1eissn
0021-9193issn
1098-5530journal_volume
158pub_type
杂志文章abstract::In order to delineate the region involved in feedback regulation of the RNA polymerase beta subunit (encoded by rpoB), a collection of rpoB-lacZ translational fusions with different endpoints both upstream and downstream of the rpoB start site was assembled on lambda phage vectors. The extent of translational repressi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7174-7179.1992
更新日期:1992-11-01 00:00:00
abstract::This work describes the draft genome sequence of Lactococcus garvieae DCC43. The 2.2-Mb draft genome contains 2,227 predicted protein-coding genes, among which is a region encoding the bacteriocin garvicin ML. No antibiotic resistance genes or capsule-related virulence genes were identified. Two plasmid replication re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01864-12
更新日期:2012-12-01 00:00:00
abstract::A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.1.11-19.1995
更新日期:1995-01-01 00:00:00
abstract::We have constructed Escherichia coli strains containing mutations at two different loci, both originally selected for failure to support lambda site-specific recombination: himA and gyrB-him(Ts). Although the gyrB-him(Ts) mutations by themselves reduce supercoiling at high temperature, the double mutants show a far gr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.484-489.1984
更新日期:1984-02-01 00:00:00
abstract::We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.13.4356-4360.1992
更新日期:1992-07-01 00:00:00
abstract::Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-tre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.732-739.1986
更新日期:1986-03-01 00:00:00
abstract::We report the draft genome sequence of methicillin-resistant Staphylococcus aureus (MRSA) strain ST672, an emerging disease clone in India, from a septicemia patient. The genome size is about 2.82 Mb with 2,485 open reading frames (ORFs). The staphylococcal cassette chromosome mec (SCCmec) element (type V) and immune ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01868-12
更新日期:2012-12-01 00:00:00
abstract::The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.249-258.1971
更新日期:1971-01-01 00:00:00
abstract::The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.175-183.1984
更新日期:1984-10-01 00:00:00
abstract::A globomycin-resistant mutant of Escherichia coli was found to produce a precursor of the major outer membrane lipoprotein (prolipoprotein), in which the glycine residue at position 14 within the signal peptide was replaced by an aspartic acid residue. The same mutation has been reported by Lin et al. (Proc. Natl. Aca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.407-411.1983
更新日期:1983-07-01 00:00:00
abstract::To examine the role of the amino acid residues (between positions 258 and 275 and positions 297 and 298) of the alpha-subunit of RNA polymerase in TyrR-mediated activation of the mtr promoter, we have carried out in vitro transcription experiments using a set of mutant RNA polymerases with a supercoiled mtr template. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6187-6191.1997
更新日期:1997-10-01 00:00:00
abstract::We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5699-5704.1991
更新日期:1991-09-01 00:00:00
abstract::Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.303-308.1992
更新日期:1992-01-01 00:00:00
abstract::Isoaspartyl sites, in which an aspartic acid residue is linked to its C-flanking neighbor via its beta-carboxyl side chain, are generally assumed to be an abnormal modification arising as proteins age. The enzyme protein L-isoaspartate methyltransferase (PIMT), present in many bacteria, plants, and animals, catalyzes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.9.2872-2877.1999
更新日期:1999-05-01 00:00:00
abstract::When the Lac- strain of Escherichia coli, FC40, is incubated with lactose as its sole carbon and energy source, Lac+ revertants arise at a constant rate, a phenomenon known as adaptive mutation. Two alternative models for adaptive mutation have been proposed: (i) recombination-dependent mutation, which specifies that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01706-06
更新日期:2007-03-01 00:00:00
abstract::Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01471-13
更新日期:2014-04-01 00:00:00
abstract::The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.2.693-701.1983
更新日期:1983-05-01 00:00:00
abstract::The gram-positive bacterium Bacillus subtilis contains two minimal Tat translocases, TatAdCd and TatAyCy, which are each involved in the secretion of one or more specific protein substrates. We have investigated the subcellular localization of the TatA components by employing C-terminal green fluorescent protein (GFP)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00215-09
更新日期:2009-07-01 00:00:00
abstract::Doxtader, K. G. (Cornell University, Ithaca, N.Y.), and M. Alexander. Role of 3-nitropropanoic acid in nitrate formation by Aspergillus flavus. J. Bacteriol. 91:1186-1191. 1966.-Aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-NPA), kojic acid, and a substance tentatively identified as N-formyl-N-hydroxy-gl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1186-1191.1966
更新日期:1966-03-01 00:00:00
abstract::We constructed a shuttle vector, pE5-2, which can replicate in both Bacteroides spp. and Escherichia coli. pE5-2 contains a cryptic Bacteroides plasmid (pB8-51), a 3.8-kilobase (kb) EcoRI-D fragment from the 41-kb Bacteroides fragilis plasmid pBF4, and RSF1010, an IncQ E. coli plasmid. pE5-2 was mobilized by R751, an ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.626-632.1985
更新日期:1985-05-01 00:00:00
abstract::Intracellular serine proteases of Bacillus licheniformis and Bacillus subtilis are closely related. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.2.1017-1019.1979
更新日期:1979-02-01 00:00:00
abstract::The mechanism of methyl-beta-D-thiogalactoside-phosphate (TMG-P) expulsion from Streptococcus pyogenes was studied. The expulsion elicited by glucose was not due to exchange vectorial transphosphorylation between the expelled TMG and the incoming glucose since more beta-galactoside was displaced than glucose taken up,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.354-361.1983
更新日期:1983-10-01 00:00:00
abstract::Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00264-20
更新日期:2020-10-22 00:00:00
abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
更新日期:1990-07-01 00:00:00
abstract::The cell envelope of gram-negative bacteria is responsible for many important biological functions: it plays a structural role, it accommodates the selective transfer of material across the cell wall, it undergoes changes made necessary by growth and division, and it transfers information about the environment into th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00132-08
更新日期:2008-06-01 00:00:00
abstract::Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.6015-6017.1995
更新日期:1995-10-01 00:00:00
abstract::Spirochetes are an evolutionary and structurally unique group of bacteria. Outermost is a membrane sheath (OS), and within this sheath are the protoplasmic cell cylinder (PC) and periplasmic flagella (PFs). The PFs are attached at each end of the PC and, depending on the species, may or may not overlap in the center o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6539-6545.1996
更新日期:1996-11-01 00:00:00
abstract::We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is positively growth rate regulated in exponentially growing bacteria and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7395-7400.1991
更新日期:1991-11-01 00:00:00
abstract::Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00212-17
更新日期:2017-06-27 00:00:00
abstract::Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5639-5647.1994
更新日期:1994-09-01 00:00:00