Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium.

abstract：:Mutations (env) which resulted in increased sensitivity of gonococci to diverse compounds were studied by transformation. Strains carrying an env mutation were more sensitive than wild-type strains to several antibiotics, dyes, and detergents. The env mutations resulted in complete phenotypic suppression of low-level ...

journal_title：Journal of bacteriology

authors： Sarubbi FA Jr,Sparling PF,Blackman E,Lewis E

更新日期：1975-11-01 00:00:00

abstract：:Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...

journal_title：Journal of bacteriology

authors： Kreuzer KN,Cozzarelli NR

更新日期：1979-11-01 00:00:00

abstract：:The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same cu...

journal_title：Journal of bacteriology

authors： Hiraga S,Jaffé A,Ogura T,Mori H,Takahashi H

更新日期：1986-04-01 00:00:00

abstract：:The suf operon is composed of four genes (sufB, sufC, sufD, and sufS) and is highly conserved in the genomes of cyanobacteria. Open reading frame sll0088 in Synechocystis sp. strain PCC 6803 is located near the 5' end of the suf operon but is transcribed in the direction opposite that of the suf operon. We previously ...

journal_title：Journal of bacteriology

authors： Wang T,Shen G,Balasubramanian R,McIntosh L,Bryant DA,Golbeck JH

更新日期：2004-02-01 00:00:00

abstract：:cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of ...

journal_title：Journal of bacteriology

authors： Ambulos NP Jr,Duvall EJ,Lovett PS

更新日期：1987-03-01 00:00:00

abstract：:Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the...

journal_title：Journal of bacteriology

authors： Toh E,Kurtz HD Jr,Brun YV

更新日期：2008-11-01 00:00:00

abstract：:The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...

journal_title：Journal of bacteriology

authors： Kew OM,Douglas HC

更新日期：1976-01-01 00:00:00

abstract：:A reversal in the relative amounts of the two major species of tyrosine transfer ribonucleic acid (tRNATyr) (I and II) has been previously observed by others during the development of Bacillus subtilis. These species have been purified by benzoylated diethylaminoethyl-cellulose chromatography and were shown to differ ...

journal_title：Journal of bacteriology

authors： Menichi B,Heyman T

更新日期：1976-07-01 00:00:00

abstract：:We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc₄-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation t...

journal_title：Journal of bacteriology

authors： Nguyen DH,Park JT,Shim JH,Tran PL,Oktavina EF,Nguyen TL,Lee SJ,Park CS,Li D,Park SH,Stapleton D,Lee JS,Park KH

更新日期：2014-06-01 00:00:00

abstract：:The enzyme phosphatidylglycerolphosphate synthase (PGPS; CDP-diacylglycerol glycerol 3-phosphate 3-phosphatidyltransferase; EC 2.7.8.5) catalyzes the committed step in the cardiolipin biosynthetic pathway. To study the regulation of PGPS in Schizosaccharomyces pombe, we characterized the enzyme biochemically. Maximum ...

journal_title：Journal of bacteriology

authors： Karkhoff-Schweizer RR,Kelly BL,Greenberg ML

更新日期：1991-10-01 00:00:00

abstract：:Injection of mice with a sublethal dose of endotoxin 2 hr after administration of 0.1 ml of carbon tetrachloride (CCl(4)) killed 75% of the animals. CCl(4) alone killed no controls. Significant protection against this effect was afforded by 5 mg of cortisone and by 5 mg of nicotinamide adenine dinucleotide (NAD). With...

journal_title：Journal of bacteriology

authors： Synder IS,Agarwal MK,Berry LJ

更新日期：1967-12-01 00:00:00

abstract：:Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by ...

journal_title：Journal of bacteriology

authors： HEDGECOCK LW,COSTELLO RL

更新日期：1962-08-01 00:00:00

abstract：:Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthes...

journal_title：Journal of bacteriology

authors： Hadden CT

更新日期：1979-07-01 00:00:00

abstract：:Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...

journal_title：Journal of bacteriology

authors： Nam SH,Choi SH,Kang A,Lee KS,Kim DW,Kim RN,Kim DS,Park HS

更新日期：2012-04-01 00:00:00

abstract：:The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...

journal_title：Journal of bacteriology

authors： Premakumar R,Sorger GJ,Gooden D

更新日期：1980-07-01 00:00:00

abstract：:Analyses of Rhizobium meliloti trp auxotrophs suggest that anthranilate biosynthesis by the R. meliloti trpE(G) gene product is necessary during nodule development for establishment of an effective symbiosis. trpE(G) mutants, as well as mutants blocked earlier along this pathway in aromatic amino acid biosynthesis, fo...

journal_title：Journal of bacteriology

authors： Barsomian GD,Urzainqui A,Lohman K,Walker GC

更新日期：1992-07-01 00:00:00

abstract：:A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the p...

journal_title：Journal of bacteriology

authors： Fukui K,Sagara Y,Yoshida N,Matsuoka T

更新日期：1969-04-01 00:00:00

abstract：:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

journal_title：Journal of bacteriology

authors： Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

更新日期：1992-05-01 00:00:00

abstract：:We examined the kinetics of beta-galactosidase (EC 3.2.1.23) induction in the yeast Kluyveromyces lactis. Enzyme activity began to increase 10 to 15 min, about 1/10 of a cell generation, after the addition of inducer and continued to increase linearly for from 7 to 9 cell generations before reaching a maximum, some 12...

journal_title：Journal of bacteriology

authors： Dickson RC,Markin JS

更新日期：1980-06-01 00:00:00

abstract：:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

journal_title：Journal of bacteriology

authors： Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

更新日期：2000-03-01 00:00:00

abstract：:The heat shock response of the groESL operon of Agrobacterium tumefaciens was studied at the RNA level. The operon was found to be activated under heat shock conditions and transcribed as a polycistronic mRNA that contains the groES and groEL genes. After activation, the polycistronic mRNA appeared to be cleaved betwe...

journal_title：Journal of bacteriology

authors： Segal G,Ron EZ

更新日期：1995-02-01 00:00:00

abstract：:Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...

journal_title：Journal of bacteriology

authors： Kwon M,Lee J,Lee C,Park C

更新日期：2012-04-01 00:00:00

abstract：:The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

journal_title：Journal of bacteriology

authors： den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

更新日期：2008-07-01 00:00:00

abstract：:Nucleotide excision repair (NER) is universally used to recognize and remove many types of DNA damage. In eubacteria, the NER system typically consists of UvrA, UvrB, UvrC, the UvrD helicase, DNA polymerase I, and ligase. In addition, when DNA damage blocks transcription, transcription-repair coupling factor (TRCF), t...

journal_title：Journal of bacteriology

authors： LeCuyer BE,Criss AK,Seifert HS

更新日期：2010-02-01 00:00:00

abstract：:Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...

journal_title：Journal of bacteriology

authors： Kanipes MI,Papp-Szabo E,Guerry P,Monteiro MA

更新日期：2006-05-01 00:00:00

abstract：:The complete nucleotide sequence of the hutH gene, encoding histidine ammonia-lyase (histidase), in Pseudomonas putida ATCC 12633 has been determined from the appropriate portions of the hut region that had been cloned into Escherichia coli. The resulting DNA sequence revealed an open reading frame of 1,530 base pairs...

journal_title：Journal of bacteriology

authors： Consevage MW,Phillips AT

更新日期：1990-05-01 00:00:00

abstract：:Sulfate transport capacity was not regulated by cysteine, methionine, or glutathione in Pseudomonas halodurans, but growth on sulfate or thiosulfate suppressed transport. Subsequent sulfur starvation of cultures grown on all sulfur sources except glutathione stimulated uptake. Only methionine failed to regulate sulfat...

journal_title：Journal of bacteriology

authors： Cuhel RL,Taylor CD,Jannasch HW

更新日期：1981-08-01 00:00:00

abstract：:The ompB operon of Escherichia coli contains the structural genes for two proteins, OmpR and EnvZ, which control the osmoregulated biosynthesis of the porin proteins OmpF and OmpC. By inserting XbaI octamer linkers into the cloned ompB locus, four distinct frameshift mutants were isolated and subsequently characterize...

journal_title：Journal of bacteriology

authors： Comeau DE,Ikenaka K,Tsung KL,Inouye M

更新日期：1985-11-01 00:00:00

abstract：:Despite the considerable knowledge of bacterial high-molecular-weight (HMW) polycyclic aromatic hydrocarbon (PAH) metabolism, the key enzyme(s) and its pleiotropic and epistatic behavior(s) responsible for low-molecular-weight (LMW) PAHs in HMW PAH-metabolic networks remain poorly understood. In this study, a phenotyp...

journal_title：Journal of bacteriology

authors： Kweon O,Kim SJ,Kim DW,Kim JM,Kim HL,Ahn Y,Sutherland JB,Cerniglia CE

更新日期：2014-10-01 00:00:00

abstract：:Eight different derivatives of R plasmid RP1 with thermosensitive mutations affecting maintenance in Escherichia coli and Pseudomonas aeruginosa were introduce into Rhizobium meliloti. None of the plasmids showed a thermosensitive character in R. meliloti. On the other hand, a certain deletion mutation in RP1 was foun...

journal_title：Journal of bacteriology

authors： Hooykaas PJ,den Dulk-Ras H,Schilperoort RA

更新日期：1982-04-01 00:00:00