Abstract:
:Mutations (env) which resulted in increased sensitivity of gonococci to diverse compounds were studied by transformation. Strains carrying an env mutation were more sensitive than wild-type strains to several antibiotics, dyes, and detergents. The env mutations resulted in complete phenotypic suppression of low-level resistance to these same drugs determined by mutation at ery. Recombination was observed in transformation crosses between various env mutants. The env locus was not linked to the cluster of antibiotic resistance genes near str and spc.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Sarubbi FA Jr,Sparling PF,Blackman E,Lewis Edoi
10.1128/JB.124.2.750-756.1975subject
Has Abstractpub_date
1975-11-01 00:00:00pages
750-6issue
2eissn
0021-9193issn
1098-5530journal_volume
124pub_type
杂志文章abstract::Kasugamycin-resistant mutants of Bacillus subtilis were isolated and classified into two groups, one of which had resistance to kasugamycin in in vitro protein synthesis and mapped in the ribosomal region. The other group had no resistance to kasugamycin in in vitro protein synthesis and had weak cross-resistance to g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.1149-1150.1978
更新日期:1978-09-01 00:00:00
abstract::In Escherichia coli, putrescine is metabolized to succinate for use as a carbon and nitrogen source by the putrescine utilization pathway (Puu pathway). One gene in the puu gene cluster encodes a transcription factor, PuuR, which has a helix-turn-helix DNA-binding motif. DNA microarray analysis of an E. coli puuR muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00097-12
更新日期:2012-07-01 00:00:00
abstract::Four regulated promoters that direct the transcription of genes (i.e., korA, tra, kilB, and korB) involved in the transfer of the Streptomyces plasmid pIJ101 were isolated following the in vitro fusion of plasmid DNA fragments to a promoterless gene encoding the S. lividans extracellular enzyme beta-galactosidase. Int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5768-5775.1989
更新日期:1989-11-01 00:00:00
abstract::A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6469-6475.1990
更新日期:1990-11-01 00:00:00
abstract::A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1087-1092.1994
更新日期:1994-02-01 00:00:00
abstract::lisRK encodes a two-component regulatory system in the food pathogen Listeria monocytogenes LO28. Following identification of the operon in an acid-tolerant Tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.21.6840-6843.1999
更新日期:1999-11-01 00:00:00
abstract::The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.4.877-882.1995
更新日期:1995-02-01 00:00:00
abstract::The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00511-19
更新日期:2019-11-20 00:00:00
abstract::The stable inheritance of the broad-host-range plasmid RK2 is due at least in part to functions within a region located at coordinates 32.8 to 35.9 kb, termed the RK2 par locus. This locus encodes four previously identified genes in two operons (parCBA and parD; M. Gerlitz, O. Hrabak, and H. Schwab, J. Bacteriol. 172:...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.24.8119-8132.1992
更新日期:1992-12-01 00:00:00
abstract::In Bacillus subtilis, extracellular peptide signaling regulates several biological processes. Secreted Phr signaling peptides are imported into the cell and act intracellularly to antagonize the activity of regulators known as Rap proteins. B. subtilis encodes several Rap proteins and Phr peptides, and the processes r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00300-06
更新日期:2006-07-01 00:00:00
abstract::The recA441 mutant of Escherichia coli, which has been thought to have thermoinducible constitutive RecA protease activity, is known to have two mutations within recA. We show here that the mutation that alters codon 38 actually confers temperature-independent constitutive protease activity; the second mutation in rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.1.407-409.1985
更新日期:1985-07-01 00:00:00
abstract::A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.976-979.1974
更新日期:1974-11-01 00:00:00
abstract::Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.404-410.1970
更新日期:1970-05-01 00:00:00
abstract::Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2724-2732.2001
更新日期:2001-05-01 00:00:00
abstract::Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5036-5042.1992
更新日期:1992-08-01 00:00:00
abstract::We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.389-398.1997
更新日期:1997-01-01 00:00:00
abstract::In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01016-07
更新日期:2007-12-01 00:00:00
abstract::The gene encoding the fimbrial subunit protein of Bacteroides gingivalis 381, fimbrilin, has been cloned and sequenced. The gene was present as a single copy on the bacterial chromosome, and the codon usage in the gene conformed closely to that expected for an abundant protein. The predicted size of the mature protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1658-1665.1988
更新日期:1988-04-01 00:00:00
abstract::Escherichia coli K-12 deficient in galactose-1-phosphate uridyl transferase is capable of converting significant amounts of d-[1-(14)C]galactose to (14)CO(2), whereas strains deficient in other enzymes of the Leloir pathway cannot do so. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.558-561.1975
更新日期:1975-10-01 00:00:00
abstract::A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2628-2636.1995
更新日期:1995-05-01 00:00:00
abstract::Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.406-414.1971
更新日期:1971-08-01 00:00:00
abstract::Organoautotrophic growth of Alcaligenes eutrophus on formate was dependent on the presence of molybdate in the medium. Supplementation of the medium with tungstate lead to growth cessation. Corresponding effects of these anions were observed for the activity of the soluble, NAD(+)-linked formate dehydrogenase (S-FDH; ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4719-4728.1993
更新日期:1993-08-01 00:00:00
abstract::Bacillus subtilis cells cannot sporulate in the presence of catabolites such as glucose. During the analysis of Tn10-generated mutants, we found that deletion of the C-terminal region of the tnrA gene, which encodes a global regulator that positively regulates a number of genes in response to nitrogen limitation, resu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.5009-5012.2000
更新日期:2000-09-01 00:00:00
abstract::Bacillus subtilis mutants with reduced rates of cell wall autolysis reached a constant rate of wall turnover after a longer lag than the standard strain but eventually showed the same turnover rate. In reverse, a turnover-deficient mutant autolysed at a slightly higher rate than the standard strain. Consequently, ther...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.318-320.1984
更新日期:1984-01-01 00:00:00
abstract::A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2521-2526.1988
更新日期:1988-06-01 00:00:00
abstract::A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4655-4666.1989
更新日期:1989-09-01 00:00:00
abstract::mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1655-1660.1994
更新日期:1994-03-01 00:00:00
abstract::The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01520-06
更新日期:2007-03-01 00:00:00
abstract::The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.264-267.1979
更新日期:1979-04-01 00:00:00
abstract::We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.859-865.1979
更新日期:1979-09-01 00:00:00