Physiological and biochemical characterization of the soluble formate dehydrogenase, a molybdoenzyme from Alcaligenes eutrophus.

abstract：:Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Lel...

journal_title：Journal of bacteriology

authors： Vaughan EE,van den Bogaard PT,Catzeddu P,Kuipers OP,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:Chloromuconate cycloisomerases of bacteria utilizing chloroaromatic compounds are known to convert 3-chloro-cis,cis-muconate to cis-dienelactone (cis-4-carboxymethylenebut-2-en-4-olide), while usual muconate cycloisomerases transform the same substrate to the bacteriotoxic protoanemonin. Formation of protoanemonin req...

journal_title：Journal of bacteriology

authors： Kaulmann U,Kaschabek SR,Schlömann M

更新日期：2001-08-01 00:00:00

abstract：:A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the...

journal_title：Journal of bacteriology

authors： Verwer RW,Nanninga N,Keck W,Schwarz U

更新日期：1978-11-01 00:00:00

abstract：:Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...

journal_title：Journal of bacteriology

authors： de Graaff J,Crosa JH,Heffron F,Falkow S

更新日期：1978-06-01 00:00:00

abstract：:The 2,647-bp nucleotide sequence of cryptic plasmid pTD1, isolated from the oral spirochete Treponema denticola, was determined. The sequence revealed two open reading frames, A and B, which encode polypeptides of 335 and 235 amino acids, respectively. Open reading frame A shows sequence similarity to genes that encod...

journal_title：Journal of bacteriology

authors： MacDougall J,Margarita D,Saint Girons I

更新日期：1992-04-01 00:00:00

abstract：:The gap-2 gene, encoding the NAD(P)-dependent D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH2) of the cyanobacterium Synechocystis sp. strain PCC 6803, was cloned by functional complementation of an Escherichia coli gap mutant with a genomic DNA library; this is the first time that this cloning strategy has been us...

journal_title：Journal of bacteriology

authors： Valverde F,Losada M,Serrano A

更新日期：1997-07-01 00:00:00

abstract：:During meiosis and sporulation in Saccharomyces cerevisiae, the recessive genetic marker for cycloheximide resistance, believed to be due to an altered ribosomal protein (C. S. McLaughlin, p. 815-827, in M. Nomura et al., ed., Ribosomes, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.), is expressed as early a...

journal_title：Journal of bacteriology

authors： Emanuel JR,Magee PT

更新日期：1981-03-01 00:00:00

abstract：:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...

journal_title：Journal of bacteriology

authors： Previte JJ

更新日期：1968-06-01 00:00:00

abstract：:The involvement of the recently characterized 5' exonuclease activity of RNase J1 and endonuclease activity of RNase Y in the turnover of ΔermC mRNA in Bacillus subtilis was investigated. Evidence is presented that both of these activities determine the half-life of ΔermC mRNA. ...

journal_title：Journal of bacteriology

authors： Yao S,Richards J,Belasco JG,Bechhofer DH

更新日期：2011-11-01 00:00:00

abstract：:CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Furth...

journal_title：Journal of bacteriology

authors： Youn H,Kerby RL,Conrad M,Roberts GP

更新日期：2004-03-01 00:00:00

abstract：UNLABELLED:Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis of the genome of the thermophilic bacterium Alicyclobacillus...

journal_title：Journal of bacteriology

authors： Ding JM,Yu TT,Han NY,Yu JL,Li JJ,Yang YJ,Tang XH,Xu B,Zhou JP,Tang HZ,Huang ZX

更新日期：2015-11-02 00:00:00

abstract：:The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is...

journal_title：Journal of bacteriology

authors： Zúñiga-Ripa A,Barbier T,Conde-Álvarez R,Martínez-Gómez E,Palacios-Chaves L,Gil-Ramírez Y,Grilló MJ,Letesson JJ,Iriarte M,Moriyón I

更新日期：2014-08-15 00:00:00

abstract：:Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. ...

journal_title：Journal of bacteriology

authors： Young C,Burlage RS,Figurski DH

更新日期：1987-03-01 00:00:00

abstract：:Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...

journal_title：Journal of bacteriology

authors： Hook-Barnard I,Johnson XB,Hinton DM

更新日期：2006-12-01 00:00:00

abstract：:This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...

journal_title：Journal of bacteriology

authors： Margalit H,Grover NB

更新日期：1987-11-01 00:00:00

abstract：:The Bacillus subtilis ureABC operon encodes homologs of the three subunits of urease enzymes of the family Enterobacteriaceae. Disruption of ureC prevented utilization of urea as a nitrogen source and resulted in a partial growth defect in minimal medium containing limiting amounts of arginine or allantoin as the sole...

journal_title：Journal of bacteriology

authors： Cruz-Ramos H,Glaser P,Wray LV Jr,Fisher SH

更新日期：1997-05-01 00:00:00

abstract：:When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...

journal_title：Journal of bacteriology

authors： Sagar V,Kumar R,Ganguly NK,Menon T,Chakraborti A

更新日期：2007-04-01 00:00:00

abstract：:Three groups of linked markers were mapped in Bacillus thuringiensis 4042B by using two-, three-, and four-factor crosses mediated by the temperate bacteriophages TP-13 and TP-18. The order of markers was (trp-11, trp-2)-(leu-1, leu-2)-his-1-(lys-1, lys-2)-cys-1 in the first group; met-1-(argCl, argOl)-met-2-(pyr-1, p...

journal_title：Journal of bacteriology

authors： Barsomian GD,Robillard NJ,Thorne CB

更新日期：1984-03-01 00:00:00

abstract：:The Salmonella typhimurium pi-histidine episome was identified by Ames et al. (2) in an unstable partial revertant of a deletion mutation-containing strain, hisG203. HisG203 lacks the histidine operator, promoter, and part of the first structural gene. In this paper, we study some properties of pi factor and demonstra...

journal_title：Journal of bacteriology

authors： Levinthal M,Yeh J

更新日期：1972-03-01 00:00:00

abstract：:Spontaneous mutators of Salmonella typhimurium LT2 were generated by inserting the transposable element Tn5 or Tn10 into the bacterial chromosome. Two mutators mapped at the position of the mutH and mutL loci of S. typhimurium, and two other mutators mapped at positions corresponding to the mutS and uvrD loci of Esche...

journal_title：Journal of bacteriology

authors： Shanabruch WG,Behlau I,Walker GC

更新日期：1981-09-01 00:00:00

abstract：:A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

journal_title：Journal of bacteriology

authors： Kelly R,Kwon-Chung KJ

更新日期：1992-01-01 00:00:00

abstract：:FNR is an Escherichia coli transcription factor that regulates the transcription of many genes in response to anaerobiosis. We have constructed a series of artificial FNR-dependent promoters, based on the melR promoter, in which a consensus FNR binding site was centered at position -41.5 relative to the transcription ...

journal_title：Journal of bacteriology

authors： Barnard AM,Green J,Busby SJ

更新日期：2003-10-01 00:00:00

abstract：:The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phe...

journal_title：Journal of bacteriology

authors： Wehrl W,Niederweis M,Schumann W

更新日期：2000-07-01 00:00:00

abstract：:In Escherichia coli, the SecB/SecA branch of the Sec pathway and the twin-arginine translocation (Tat) pathway represent two alternative possibilities for posttranslational translocation of proteins across the cytoplasmic membrane. Maintenance of pathway specificity was analyzed using a model precursor consisting of t...

journal_title：Journal of bacteriology

authors： Blaudeck N,Kreutzenbeck P,Freudl R,Sprenger GA

更新日期：2003-05-01 00:00:00

abstract：:A reversal in the relative amounts of the two major species of tyrosine transfer ribonucleic acid (tRNATyr) (I and II) has been previously observed by others during the development of Bacillus subtilis. These species have been purified by benzoylated diethylaminoethyl-cellulose chromatography and were shown to differ ...

journal_title：Journal of bacteriology

authors： Menichi B,Heyman T

更新日期：1976-07-01 00:00:00

abstract：:A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...

journal_title：Journal of bacteriology

authors： Malki S,Saimmaime I,De Luca G,Rousset M,Dermoun Z,Belaich JP

更新日期：1995-05-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell mem...

journal_title：Journal of bacteriology

authors： Wakim BT,Uffen RL

更新日期：1983-01-01 00:00:00

abstract：:The virulence of Pseudomonas aeruginosa and other surface pathogens involves the coordinate expression of a wide range of virulence determinants, including type IV pili. These surface filaments are important for the colonization of host epithelial tissues and mediate bacterial attachment to, and translocation across, ...

journal_title：Journal of bacteriology

authors： Leech AJ,Mattick JS

更新日期：2006-12-01 00:00:00

abstract：:The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR...

journal_title：Journal of bacteriology

authors： Choi SH,Greenberg EP

更新日期：1992-06-01 00:00:00