Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli.

Abstract:

:Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter situation. Plasmid-mediated elevation in tryptophanyl-tRNA synthetase reversed the inhibitory effect of indolmycin. Indolmycin did not facilitate the binding of purified Trp repressor protein to trp operator DNA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bogosian G,Haydock PV,Somerville RL

doi

10.1128/JB.153.2.1120-1123.1983

subject

Has Abstract

pub_date

1983-02-01 00:00:00

pages

1120-3

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

153

pub_type

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