Repression of the Staphylococcus aureus accessory gene regulator in serum and in vivo.

Abstract:

:Subgenomic DNA microarrays were employed to evaluate the expression of the accessory gene regulator (agr locus) as well as multiple virulence-associated genes in Staphylococcus aureus. Gene expression was examined during growth of S. aureus in vitro in standard laboratory medium and rabbit serum and in vivo in subcutaneous chambers implanted in either nonimmune rabbits or rabbits immunized with staphylococcal enterotoxin B. Expression of RNAIII, the effector molecule of the agr locus, was dramatically repressed in serum and in vivo, despite the increased expression of secreted virulence factors sufficient to cause toxic shock syndrome (TSS) in the animals. Statistical analysis and clustering of virulence genes based on their expression profiles in the various experimental conditions demonstrated no positive correlation between the expression of agr and any staphylococcal virulence factors examined. Disruption of the agr locus had only a minimal effect on the expression in vivo of the virulence factors examined. An effect of immunization on the expression of agr and virulence factors was also observed. These results suggest that agr activation is not necessary for development of staphylococcal TSS and that regulatory circuits responding to the in vivo environment override agr activity.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yarwood JM,McCormick JK,Paustian ML,Kapur V,Schlievert PM

doi

10.1128/jb.184.4.1095-1101.2002

keywords:

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

1095-101

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

184

pub_type

杂志文章
  • Identification of a regulator that controls stationary-phase expression of catalase-peroxidase in Caulobacter crescentus.

    abstract::Expression of the catalase-peroxidase of Caulobacter crescentus, a gram-negative member of the alpha subdivision of the Proteobacteria, is 50-fold higher in stationary-phase cultures than in exponential cultures. To identify regulators of the starvation response, Tn5 insertion mutants were isolated with reduced expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.19.6152-6159.1999

    authors: Rava PS,Somma L,Steinman HM

    更新日期:1999-10-01 00:00:00

  • Importance of cis determinants and nitrogenase activity in regulated stability of the Klebsiella pneumoniae nitrogenase structural gene mRNA.

    abstract::The Klebsiella pneumoniae nitrogen fixation (nif) mRNAs are unusually stable, with half-lives of 20 to 30 min under conditions favorable to nitrogen fixation (limiting nitrogen, anaerobiosis, temperatures of 30 degrees C). Addition of O2 or fixed nitrogen or temperature increases to 37 degrees C or more result in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.12.3751-3760.1999

    authors: Simon HM,Gosink MM,Roberts GP

    更新日期:1999-06-01 00:00:00

  • Tungsten transport protein A (WtpA) in Pyrococcus furiosus: the first member of a new class of tungstate and molybdate transporters.

    abstract::A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00548-06

    authors: Bevers LE,Hagedoorn PL,Krijger GC,Hagen WR

    更新日期:2006-09-01 00:00:00

  • Enzymatic specificities and modes of action of the two catalytic domains of the XynC xylanase from Fibrobacter succinogenes S85.

    abstract::The xylanase XynC of Fibrobacter succinogenes S85 was recently shown to contain three distinct domains, A, B, and C (F. W. Paradis, H. Zhu, P. J. Krell, J. P. Phillips, and C. W. Forsberg, J. Bacteriol. 175:7666-7672, 1993). Domains A and B each bear an active site capable of hydrolyzing xylan, while domain C has no e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.13.3885-3894.1994

    authors: Zhu H,Paradis FW,Krell PJ,Phillips JP,Forsberg CW

    更新日期:1994-07-01 00:00:00

  • Regulation of sialic acid metabolism in Escherichia coli: role of N-acylneuraminate pyruvate-lyase.

    abstract::In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.854-860.1985

    authors: Vimr ER,Troy FA

    更新日期:1985-11-01 00:00:00

  • A Sorangium cellulosum (myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A: cloning, characterization, and homology to polyketide synthase genes from actinomycetes.

    abstract::A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.13.3673-3679.1995

    authors: Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

    更新日期:1995-07-01 00:00:00

  • Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast.

    abstract::Using an auxotrophic strain of Saccharomyces cerevisiae, we examined the kinetics of ribonucleic acid (RNA) synthesis following inhibition of protein synthesis caused by amino acid starvation or cycloheximide. Removal of a required amino acid immediately stopped net protein synthesis. After a brief lag, RNA synthesis ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.773-779.1972

    authors: Roth RM,Dampier C

    更新日期:1972-02-01 00:00:00

  • Biosynthesis of branched-chain amino acids in yeast: regulation of leucine biosynthesis in prototrophic and leucine auxotrophic strains.

    abstract::The first enzyme in the biosynthesis of leucine in yeast, alpha-isopropylmalate synthetase, is inhibited by l-leucine. In a mutant resistant to the analogue 5',5',5'-trifluoroleucine, the enzyme is markedly resistant to inhibition by l-leucine. Growth ing the presence of exogenous l-leucine results in repression of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.6.2018-2024.1968

    authors: Satyanarayana T,Umbarger HE,Lindegren G

    更新日期:1968-12-01 00:00:00

  • Efficient turnover of chlorocatechols is essential for growth of Ralstonia eutropha JMP134(pJP4) in 3-chlorobenzoic acid.

    abstract::Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.5.1534-1542.2003

    authors: Pérez-Pantoja D,Ledger T,Pieper DH,González B

    更新日期:2003-03-01 00:00:00

  • Effect of glutamate on exogenous citrate catabolism of Neisseria meningitidis and of other species of Neisseria.

    abstract::Resting cell suspensions of Neisseria meningitidis group B (strain 2091) do not catabolize citrate as the sole substrate to an appreciable degree. When another substrate, such as glutamate, is also present to furnish energy for transport, citrate metabolism is greatly stimulated. Within limits, the amount of CO(2) pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.819-823.1971

    authors: Hill JC

    更新日期:1971-06-01 00:00:00

  • Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae.

    abstract::Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.2.616-619.1975

    authors: Ballou DL

    更新日期:1975-08-01 00:00:00

  • The elastic properties of the caulobacter crescentus adhesive holdfast are dependent on oligomers of N-acetylglucosamine.

    abstract::The aquatic bacterium Caulobacter crescentus attaches to solid surfaces through an adhesive holdfast located at the tip of its polar stalk, a thin cylindrical extension of the cell membrane. In this paper, the elastic properties of the C. crescentus stalk and holdfast assembly were studied by using video light microsc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.257-265.2005

    authors: Li G,Smith CS,Brun YV,Tang JX

    更新日期:2005-01-01 00:00:00

  • Functional characterization of a novel ArgA from Mycobacterium tuberculosis.

    abstract::The Mycobacterium tuberculosis gene Rv2747 encodes a novel 19-kDa ArgA that catalyzes the initial step in L-arginine biosynthesis, namely the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. Initial velocity studies reveal that Rv2747 proceeds through a sequential kinetic mechanism, with K(m) values of 280 mM ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.9.3039-3044.2005

    authors: Errey JC,Blanchard JS

    更新日期:2005-05-01 00:00:00

  • Isolation and characterization of symbiotic mutants of bradyrhizobium sp. (Arachis) strain NC92: mutants with host-specific defects in nodulation and nitrogen fixation.

    abstract::Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.2177-2186.1987

    authors: Wilson KJ,Anjaiah V,Nambiar PT,Ausubel FM

    更新日期:1987-05-01 00:00:00

  • Induced sensitization of normal laboratory animals to Brucella abortus endotoxin.

    abstract::A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.2.286-290.1968

    authors: Freedman HH,Fox AE,Willis RS,Schwartz BS

    更新日期:1968-02-01 00:00:00

  • DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a phage-tail-like bacteriocin of phytopathogenic Erwinia carotovora subsp. carotovora Er.

    abstract::Carotovoricin Er is a phage-tail-like bacteriocin produced by Erwinia carotovora subsp. carotovora strain Er, a causative agent for soft rot disease in plants. Here we studied binding and killing spectra of carotovoricin Er preparations for various strains of the bacterium (strains 645Ar, EC-2, N786, and P7) and found...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.21.6274-6281.2001

    authors: Nguyen HA,Tomita T,Hirota M,Kaneko J,Hayashi T,Kamio Y

    更新日期:2001-11-01 00:00:00

  • Genetic and biochemical characterization of a high-affinity betaine uptake system (BusA) in Lactococcus lactis reveals a new functional organization within bacterial ABC transporters.

    abstract::The cytoplasmic accumulation of exogenous betaine stimulates the growth of Lactococcus lactis cultivated under hyperosmotic conditions. We report that L. lactis possesses a single betaine transport system that belongs to the ATP-binding cassette (ABC) superfamily of transporters. Through transposon mutagenesis, a muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6238-6246.1999

    authors: Obis D,Guillot A,Gripon JC,Renault P,Bolotin A,Mistou MY

    更新日期:1999-10-01 00:00:00

  • Localization of the immunity protein-reactive domain in unmodified and chemically modified COOH-terminal peptides of colicin E1.

    abstract::The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.237-244.1985

    authors: Bishop LJ,Bjes ES,Davidson VL,Cramer WA

    更新日期:1985-10-01 00:00:00

  • Analysis of autolysins in temperature-sensitive morphological mutants of Bacillus subtilis.

    abstract::The content and distribution of autolysin were measured in temperature-sensitive morphological mutants of Bacillus subtilis. Strains RUB1000 and RUB1012 grew as rods at 30 C. At 45 C the mutants contained disproportionately less teichoic acid than peptidoglycan and grew as irregular spheres. The amount of enzyme that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.166-173.1976

    authors: Brown WC,Wilson CR,Lukehart S,Young FE,Shiflett MA

    更新日期:1976-01-01 00:00:00

  • Complementation of the lytD1 mutation of Escherichia coli by either the cI or cro gene of bacteriophage lambda.

    abstract::The lytD1 mutant of Escherichia coli exhibits temperature-sensitive growth which is attributed to cellular autolysis at the restrictive temperature. Either of two cloned phage lambda genes, identified as cI and cro, suppressed the lytD1(Ts) lysis phenotype, suggesting that lytD encodes a DNA-binding protein with a DNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.893-895.1991

    authors: Dai DX,Ishiguro EE

    更新日期:1991-01-01 00:00:00

  • DNA binding: a novel function of Pseudomonas aeruginosa type IV pili.

    abstract::The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1455-1464.2005

    authors: van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

    更新日期:2005-02-01 00:00:00

  • The Haemophilus influenzae dprABC genes constitute a competence-inducible operon that requires the product of the tfoX (sxy) gene for transcriptional activation.

    abstract::We previously showed that dprA is required for efficient processing of linear DNA during cellular transformation in Haemophilus influenzae. In this study the transcriptional regulation of dprA and two downstream genes, dprB and dprC, is examined. We demonstrate by Northern blot analysis that the dprABC genes are trans...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.15.4815-4820.1997

    authors: Karudapuram S,Barcak GJ

    更新日期:1997-08-01 00:00:00

  • Determination of genome size, macrorestriction pattern polymorphism, and nonpigmentation-specific deletion in Yersinia pestis by pulsed-field gel electrophoresis.

    abstract::Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.7.2078-2086.1992

    authors: Lucier TS,Brubaker RR

    更新日期:1992-04-01 00:00:00

  • Competence beyond Genes: Filling in the Details of the Pneumococcal Competence Transcriptome by a Systems Approach.

    abstract::DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen Streptococcus pneumoniae A study reported in this issue (J. Slager, R. Aprianto, and J.-W. Veening, J. Bacteriol. 201:e00780-18, https://doi.org/10.1128/JB.007...

    journal_title:Journal of bacteriology

    pub_type: 评论,杂志文章

    doi:10.1128/JB.00238-19

    authors: Winkler ME,Morrison DA

    更新日期:2019-06-10 00:00:00

  • Meeting report on the ASM Conference on Mechanisms of Interbacterial Cooperation and Competition.

    abstract::The ASM Conference on Mechanisms of Interbacterial Cooperation and Competition was held in Washington DC, from 1 to 4 March 2017. The conference provided an international forum for sociomicrobiologists from different disciplines to present and discuss new findings. The meeting covered a wide range of topics, spanning ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00403-17

    authors: Lories B,Parijs I,Foster KR,Steenackers HP

    更新日期:2017-08-14 00:00:00

  • Ribosomal features essential for tna operon induction: tryptophan binding at the peptidyl transferase center.

    abstract::Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01869-06

    authors: Cruz-Vera LR,New A,Squires C,Yanofsky C

    更新日期:2007-04-01 00:00:00

  • Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima.

    abstract::Edwards, P. R. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), R. Sakazaki, and I. Kato. Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima. J. Bacteriol. 84:99-103. 1962-Study of cultures of Salmonella mikawashima isolated from reptiles revealed that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.1.99-103.1962

    authors: EDWARDS PR,SAKAZAKI R,KATO I

    更新日期:1962-07-01 00:00:00

  • Stability of ribosomes and ribosomal ribonucleic acid from Bacillus stearothermophilus.

    abstract::After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1521-1526.1967

    authors: Friedman SM,Axel R,Weinstein IB

    更新日期:1967-05-01 00:00:00

  • A mutation of the transactivation gene of satellite bacteriophage P4 that suppresses the rpoA109 mutation of Escherichia coli.

    abstract::Satellite bacteriophage P4 requires the products of the late genes of a helper such as P2 in order to grow lytically. The Escherichia coli rpoA109 mutation, which alters the alpha subunit of RNA polymerase, prevents transcription of the late genes of bacteriophage P2. Suppressor mutations that define the P2 ogr gene o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3541-3548.1990

    authors: Halling C,Sunshine MG,Lane KB,Six EW,Calendar R

    更新日期:1990-07-01 00:00:00

  • Entrainment and temperature dependence of the response oscillator in Halobacterium halobium.

    abstract::Halobacterium halobium spontaneously reverses its swimming direction with a frequency of about 0.1 s-1. The self-sustained periodicity can be entrained by rhythmic light stimuli within a limited range between 0.13 and 0.3 s-1. Increase of temperature increases the reversal frequency and shortens the time to reach maxi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.2.689-692.1986

    authors: Schimz A,Hildebrand E

    更新日期:1986-05-01 00:00:00