Selection of succinic dehydrogenase mutants of Neurospora crassa.

Abstract:

:A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinate and phenazine methosulfate. Wild-type colonies reduced the dye and turned blue, whereas mutant colonies remained colorless. In this communication we present studies of a mutant, SDH-1, isolated by this method. The mutant had 18% of the succinic dehydrogenase activity of the parent strain used in the mutation experiments as determined from the ratio of Vmax activities obtained from Lineweaver-Burk plots. The SDH-1 mutant segregated in a Mendelian manner when back-crossed to its parent strain. Succinate oxidase activity in SDH-1 was low and was markedly inhibited by adenosine 5'-diphosphate. The succinate oxidase activity of the parent strain was high and was not affected by the presence of adenosine 5'-diphosphate.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Edwards DL,Belsole DM,Guzik HJ,Unger BW

doi

10.1128/JB.137.2.900-904.1979

keywords:

subject

Has Abstract

pub_date

1979-02-01 00:00:00

pages

900-4

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

137

pub_type

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