Abstract:
:Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by injecting rabbits with purified sheath material from S. natans, fractionating the serum with ammonium sulfate, and conjugating the globulin fraction with fluorescein isothiocyanate. To follow sheath formation, S. natans was grown on slides immersed in Stokes' medium at 28 C for 9 hr, and was reacted with labeled antibody. The slide cultures were then washed to remove unbound antibody, and were incubated further in fresh medium. Samples were removed at intervals and examined by fluorescence microscopy. Old portions of the sheath remained discretely labeled with no diminution in intensity of fluorescence, whereas non-fluorescent new sheath material appeared at the ends of the filaments. These results indicate that sheath synthesis does not take place by intussusception or diffuse intercalation, but by linear extension of pre-existing sheath. This interpretation was confirmed by a reverse procedure, whereby old sheath was reacted with unlabeled antibody, and new sheath was identified by addition of labeled antibody after further incubation. In this procedure, fluorescence was most intense at the growing tips of the filaments.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
ROMANO AH,GEASON DJdoi
10.1128/JB.88.4.1145-1150.1964keywords:
subject
Has Abstractpub_date
1964-10-01 00:00:00pages
1145-50eissn
0021-9193issn
1098-5530journal_volume
88pub_type
杂志文章abstract::Spores were isolated from the filamentous cyanobacterium Anabaena cylindrica, and their deoxyribonucleic acid, ribonucleic acid, and protein compositions were determined. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1154-1155.1977
更新日期:1977-02-01 00:00:00
abstract::Outer membrane preparations of Bdellovibrio bacteriovorus grown intraperiplasmically on Escherichia coli containing OmpF were prepared by the Triton X-100 procedure of Schnaitman (J. Bacteriol. 108:545-552, 1971). They contained a protein that migrated to almost the same position as E. coli OmpF in sodium dodecyl sulf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.595-599.1985
更新日期:1985-08-01 00:00:00
abstract::With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.492-500.1977
更新日期:1977-01-01 00:00:00
abstract::In cyanobacteria, gene expression is regulated mainly at the level of transcription initiation, which is mediated by the RNA polymerase holoenzyme. The RNA polymerase core is catalytically active, while the sigma factor recognizes promoter sequences. Group 2 sigma factors are similar to the principal sigma factor but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00132-09
更新日期:2009-06-01 00:00:00
abstract::The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.14.5019-5022.2005
更新日期:2005-07-01 00:00:00
abstract::The two highly homologous cbb operons of Alcaligenes eutrophus H16 that are located on the chromosome and on megaplasmid pHG1 contain genes encoding several enzymes of the Calvin carbon reduction cycle. Sequence analysis of a region from the promoter-distal part revealed two open reading frames, designated cbbT and cb...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7329-7340.1993
更新日期:1993-11-01 00:00:00
abstract::Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1621-1623.1975
更新日期:1975-12-01 00:00:00
abstract::An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5753-5761.2004
更新日期:2004-09-01 00:00:00
abstract::Weiss, K. F. (Iowa State University, Ames), J. C. Ayres, and A. A. Kraft. Inhibitory action of selenite on Escherichia coli, Proteus vulgaris, and Salmonella thompson. J. Bacteriol. 90:857-862. 1965.-The resistance of three microorganisms, Escherichia coli (ISU-41), Proteus vulgaris (ISU-37c), and Salmonella thompson ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.4.857-862.1965
更新日期:1965-10-01 00:00:00
abstract::"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00137-06
更新日期:2006-06-01 00:00:00
abstract::DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.214-220.1982
更新日期:1982-04-01 00:00:00
abstract::Mutation of a conserved His-157 in the second pore loop of KtrB drastically reduced the activity of the K+ transporter from Synechocystis sp. strain PCC 6803. This result suggests that His-157 plays an essential role in the K+ transport activity of the transporter system. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00886-06
更新日期:2006-11-01 00:00:00
abstract::The suf operon is composed of four genes (sufB, sufC, sufD, and sufS) and is highly conserved in the genomes of cyanobacteria. Open reading frame sll0088 in Synechocystis sp. strain PCC 6803 is located near the 5' end of the suf operon but is transcribed in the direction opposite that of the suf operon. We previously ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.956-967.2004
更新日期:2004-02-01 00:00:00
abstract::The yeast-phase cell cycle of Wangiella dermatitidis was studied using flow microfluorimetry and the deoxyribonucleic acid (DNA) synthesis inhibitor hydroxyurea (HU). Exposure of exponential-phase yeastlike cells to 0.1 M HU for 3 to 6 h resulted in the arrest of the cells in DNA synthesis and produced a nearly homoge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.721-731.1980
更新日期:1980-11-01 00:00:00
abstract::The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.20.5885-5892.2000
更新日期:2000-10-01 00:00:00
abstract::The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.273-277.1967
更新日期:1967-01-01 00:00:00
abstract::The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6580-6589.1992
更新日期:1992-10-01 00:00:00
abstract::Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1437-1443.1969
更新日期:1969-03-01 00:00:00
abstract::The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.1.250-258.1968
更新日期:1968-07-01 00:00:00
abstract::Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and hmw1, the products of which are known to be essential for cytadherence, the rpsD gene, and six open reading frames of un...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.4978-4985.1999
更新日期:1999-08-01 00:00:00
abstract::The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.2.443-448.1975
更新日期:1975-08-01 00:00:00
abstract::Lazaroff, Norman (British Columbia Research Council, Vancouver, B.C., Canada). Sulfate requirement for iron oxidation by Thiobacillus ferrooxidans. J. Bacteriol. 85:78-83. 1963.-The growth of Thiobacillus ferrooxidans is initially inhibited in media containing ferrous chloride in place of ferrous sulfate. This inhibit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.1.78-83.1963
更新日期:1963-01-01 00:00:00
abstract::During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.10.3470-3476.2006
更新日期:2006-05-01 00:00:00
abstract::Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00485-08
更新日期:2008-11-01 00:00:00
abstract::Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.2.676-681.1972
更新日期:1972-11-01 00:00:00
abstract::The effects of starving Escherichia coli for glucose and required amino acids were determined. The disappearance of the majority of the ribosomal population, particularly of the ribonucleic acid (RNA) moiety, was noted. The events during recovery are detailed, with emphasis placed on the timing, requirements, and cont...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.1030-1039.1968
更新日期:1968-03-01 00:00:00
abstract::gldA, the structural gene for the NAD(+)-dependent glycerol dehydrogenase, was mapped at 89.2 min on the Escherichia coli linkage map, cotransducible with, but not adjacent to, the glpFKX operon encoding the proteins for the uptake and phosphorylation of glycerol. gldA was cloned, and its position on the physical map ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1796-1800.1994
更新日期:1994-03-01 00:00:00
abstract::After frog auricles have been in contact with a suspension of bacteria or bacteria-free supernatant fluid, newly synthesized bacterial ribonucleic acid (RNA) is recovered in animal cells. It appears that the presence of bacterial deoxyribonucleic acid (DNA)-dependent RNA polymerase is necessary for the transcription o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.114-120.1973
更新日期:1973-04-01 00:00:00
abstract::The distribution of the extracellular enzyme, thiaminase I, was determined for logarithmically growing cultures of Bacillus thiaminolyticus. About 60% of the enzyme is associated with the cells throughout the growth cycle. The remainder of the enzyme is in the culture medium. The release of the cell-bound thiaminase I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.949-956.1973
更新日期:1973-09-01 00:00:00
abstract::We used a Southern hybridization assay to locate precisely the sites at which DNA replication is arrested in the terminus region of the Escherichia coli chromosome. The assay was based on the properties of restriction fragments that contain stalled replication forks. Replication forks that entered the terminus from th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4293-4298.1988
更新日期:1988-09-01 00:00:00