Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.

Abstract:

:DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was cloned on plasmid pBR322. By using recombination in vitro, we mapped the GlnC mutation to a region between glnA and glnG. This region defines a gene, glnL, which codes for a trans-acting product; the GlnC mutant produces an altered product. The glnL product plays a key role in the communication of information concerning the quality and abundance of the nitrogen source in the growth medium to a destination responsible for the regulation of glnA and other genes for enzymes responsible for nitrogen utilization.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Chen YM,Backman K,Magasanik B

doi

10.1128/JB.150.1.214-220.1982

subject

Has Abstract

pub_date

1982-04-01 00:00:00

pages

214-20

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

150

pub_type

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