Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein.

abstract：:Previous work has shown that lethal heat treatment of Bacillus subtilis spores lacking the major DNA-binding proteins SASP-alpha and -beta (alpha-beta- spores) causes significant DNA damage, including many single-strand breaks. In this work we have used a reagent specific for aldehydes present in abasic sites in DNA t...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1994-04-01 00:00:00

abstract：:Short sequence repeats (SSRs), potentially representing variable numbers of tandem repeat (VNTR) loci, were identified for the human-pathogenic yeast species Candida albicans by computerized DNA sequence scanning. The individual SSR regions were investigated in different clinical isolates of C. albicans. Most of the C...

journal_title：Journal of bacteriology

authors： Lunel FV,Licciardello L,Stefani S,Verbrugh HA,Melchers WJ,Meis JF,Scherer S,van Belkum A

更新日期：1998-08-01 00:00:00

abstract：:The flagellar regulon of Brucella melitensis 16M contains 31 genes clustered in three loci on the small chromosome. These genes encode a polar sheathed flagellum that is transiently expressed during vegetative growth and required for persistent infection in a mouse model. By following the expression of three flagellar...

journal_title：Journal of bacteriology

authors： Léonard S,Ferooz J,Haine V,Danese I,Fretin D,Tibor A,de Walque S,De Bolle X,Letesson JJ

更新日期：2007-01-01 00:00:00

abstract：:The structure of sheathed flagella from Bdellovibrio bacteriovorus was investigated. The first three periods of these flagella were characterized by progressively smaller wavelengths and amplitudes in periods more distal to the cell. The damped appearance was due to a single nonrandom transition between two helical st...

journal_title：Journal of bacteriology

authors： Thomashow LS,Rittenberg SC

更新日期：1985-09-01 00:00:00

abstract：:Spiralin could not be solubilized in the absence of detergents, and it was shown by charge-shift crossed immunoelectrophoresis that this protein was capable of binding detergents under nondenaturing conditions. These properties indicate the amphiphilic nature of spiralin, which therefore should be regarded as an intri...

journal_title：Journal of bacteriology

authors： Wróblewski H

更新日期：1979-11-01 00:00:00

abstract：:For Escherichia coli, growth on acetate requires the induction of the enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase. The branch point between the glyoxylate bypass and the Krebs cycle is controlled by phosphorylation of isocitrate dehydrogenase (IDH), inhibiting that enzyme's activity and thus...

journal_title：Journal of bacteriology

authors： Ikeda T,LaPorte DC

更新日期：1991-03-01 00:00:00

abstract：:Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...

journal_title：Journal of bacteriology

authors： Faelen M,Toussaint A

更新日期：1978-11-01 00:00:00

abstract：:Maleylacetate reductase (EC 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. Several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precu...

journal_title：Journal of bacteriology

authors： Kaschabek SR,Reineke W

更新日期：1995-01-01 00:00:00

abstract：:sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...

journal_title：Journal of bacteriology

authors： Peters HK 3rd,Carlson HC,Haldenwang WG

更新日期：1992-07-01 00:00:00

abstract：:We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...

journal_title：Journal of bacteriology

authors： Ucker DS,Signer ER

更新日期：1978-12-01 00:00:00

abstract：:Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Detection and persistence of Vi antigen in tissues of actively immunized mice. J. Bacteriol. 89:776-781. 1965.-The presence, distribution, and persistence of Vi antigen in mouse tissue was determined by me...

journal_title：Journal of bacteriology

authors： GAINES S,CURRIE JA,TULLY JG

更新日期：1965-03-01 00:00:00

abstract：:Qualitatively, the protein and fatty acid composition of purified mesosomal vesicles and the plasma membrane isolated from Staphylococcus aureus ATCC 6538P are identical, the major difference between these two cellular components being only quantitative in nature. Mesosomal vesicles and plasma membranes, when subjecte...

journal_title：Journal of bacteriology

authors： Theodore TS,Panos C

更新日期：1973-11-01 00:00:00

abstract：:Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...

journal_title：Journal of bacteriology

authors： Feeney MA,Ke N,Beckwith J

更新日期：2012-03-01 00:00:00

abstract：:A set of Saccharomyces cerevisiae strains with variable expression of only the high-affinity Hxt7 glucose transporter was constructed by partial deletion of the HXT7 promoter in vitro and integration of the gene at various copy numbers into the genome of an hxt1-7 gal2 deletion strain. The glucose transport capacity i...

journal_title：Journal of bacteriology

authors： Ye L,Kruckeberg AL,Berden JA,van Dam K

更新日期：1999-08-01 00:00:00

abstract：:Peptidoglycan was prepared from purified Bacillus subtilis spores of wild-type and several mutant strains. Digestion with muramidase resulted in cleavage of the glycosidic bonds adjacent to muramic acid replaced by peptide or alanine side chains but not the bonds adjacent to muramic lactam. Reduction of the resulting ...

journal_title：Journal of bacteriology

authors： Popham DL,Helin J,Costello CE,Setlow P

更新日期：1996-11-01 00:00:00

abstract：:Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...

journal_title：Journal of bacteriology

authors： Yamanaka K,Inouye M

更新日期：2001-05-01 00:00:00

abstract：:An inducible phosphoenolpyruvate:fructose phosphotransferase system has been detected in Azospirillum brasilense, which requires a minimum of two components of the crude extracts for activity: (i) a soluble fraction (enzyme I) and (ii) a membrane fraction (enzyme II). The uninduced cells neither show any uptake of fru...

journal_title：Journal of bacteriology

authors： Gupta KD,Ghosh S

更新日期：1984-12-01 00:00:00

abstract：:We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

journal_title：Journal of bacteriology

authors： Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

更新日期：1996-06-01 00:00:00

abstract：:The yeast-phase cell cycle of Wangiella dermatitidis was studied using flow microfluorimetry and the deoxyribonucleic acid (DNA) synthesis inhibitor hydroxyurea (HU). Exposure of exponential-phase yeastlike cells to 0.1 M HU for 3 to 6 h resulted in the arrest of the cells in DNA synthesis and produced a nearly homoge...

journal_title：Journal of bacteriology

authors： Roberts RL,Szaniszlo PJ

更新日期：1980-11-01 00:00:00

abstract：:Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeled Mycoplasma histotropicum was found related to and perhaps best classified as M. pul...

journal_title：Journal of bacteriology

authors： Kenny GE

更新日期：1969-06-01 00:00:00

abstract：:Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...

journal_title：Journal of bacteriology

authors： Gillin FD,Ganesan AT

更新日期：1975-09-01 00:00:00

abstract：:Whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. A recent report implied that not only may the former be true but also, in fact, the clearly distinct Campylobacter jejuni and Campylobacter coli species may...

journal_title：Journal of bacteriology

authors： Caro-Quintero A,Rodriguez-Castaño GP,Konstantinidis KT

更新日期：2009-09-01 00:00:00

abstract：:A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...

journal_title：Journal of bacteriology

authors： Bankaitis VA,Kline EL

更新日期：1981-08-01 00:00:00

abstract：:Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...

journal_title：Journal of bacteriology

authors： Rothstein DM,Lazinski D,Osburne MS,Sonenshein AL

更新日期：2017-06-27 00:00:00

abstract：:Fourteen bacterial strains of marine origin, which formed colonies containing crystals of a blue-black pigment, were submitted to a morphological, physiological, and nutritional characterization. The results indicated that these organisms form a new species of the genus Beneckea. ...

journal_title：Journal of bacteriology

authors： Baumann P,Baumann L,Mandel M,Allen RD

更新日期：1971-12-01 00:00:00

abstract：:Campylobacter jejuni, a spiral-shaped gram-negative bacterium, is a leading bacterial cause of human food-borne illness. Acute disease is associated with C. jejuni invasion of the intestinal epithelium. Further, maximal host cell invasion requires the secretion of proteins termed Campylobacter invasion antigens (Cia)....

journal_title：Journal of bacteriology

authors： Malik-Kale P,Parker CT,Konkel ME

更新日期：2008-04-01 00:00:00

abstract：:Bacillus subtilis strains containing deletions in the genes coding for one or two of the major small, acid-soluble spore proteins (SASP; termed SASP-alpha and SASP-beta) were constructed. These mutants sporulated normally, but the spores lacked either SASP-alpha, SASP-beta, or both proteins. The level of minor SASP di...

journal_title：Journal of bacteriology

authors： Mason JM,Setlow P

更新日期：1986-07-01 00:00:00

abstract：:To define additional components of the export machinery of Escherichia coli, I have isolated extragenic suppressors of a mutant [secA(Ts)] that is temperature sensitive for growth and secretion at 37 degrees C. Suppressors that restored growth at 37 degrees C, but that rendered the cell cold sensitive for growth at 28...

journal_title：Journal of bacteriology

authors： Oliver DB

更新日期：1985-01-01 00:00:00

abstract：:A mutant sensitive to colicin M at 30 degrees C and tolerant at 42 degrees C to high concentrations of colicin M was isolated from Escherichia coli K-12. A temperature shift from 30 to 42 degrees C rescued all cells up to the time they started to lyse at 30 degrees C (25 min after addition of colicin M). The growth ra...

journal_title：Journal of bacteriology

authors： Schaller K,Krauel A,Braun V

更新日期：1981-07-01 00:00:00

abstract：:The toxR gene of Vibrio cholerae encodes a transcriptional activator required for the expression of the cholera toxin genes (ctxAB) and more than 15 other genes encoding secreted or membrane proteins. The latter group includes virulence genes involved in the biogenesis of the TCP pilus, the accessory colonization fact...

journal_title：Journal of bacteriology

authors： Parsot C,Mekalanos JJ

更新日期：1991-05-01 00:00:00