Transductional analysis of Pseudomonas aeruginosa methionineless auxotrophs.

abstract：:A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...

journal_title：Journal of bacteriology

authors： Weiss RL

更新日期：1976-11-01 00:00:00

abstract：:A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein...

journal_title：Journal of bacteriology

authors： de Crecy-Lagard V,Glaser P,Lejeune P,Sismeiro O,Barber CE,Daniels MJ,Danchin A

更新日期：1990-10-01 00:00:00

abstract：:The sets of compounds that can support growth of an organism are defined by the presence of transporters and metabolic pathways that convert nutrient sources into cellular components and energy for growth. A collection of known nutrient sources can therefore serve both as an impetus for investigating new metabolic pat...

journal_title：Journal of bacteriology

authors： Mackie A,Paley S,Keseler IM,Shearer A,Paulsen IT,Karp PD

更新日期：2014-03-01 00:00:00

abstract：:A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

journal_title：Journal of bacteriology

authors： Baum JA

更新日期：1994-05-01 00:00:00

abstract：:Temperate bacteriophages effect chromosomal evolution of their bacterial hosts, mediating rearrangements and the acquisition of novel genes from other taxa. Although the Haemophilus influenzae genome shows evidence of past phage-mediated lateral transfer, the phages presumed responsible have not been identified. To da...

journal_title：Journal of bacteriology

authors： Williams BJ,Golomb M,Phillips T,Brownlee J,Olson MV,Smith AL

更新日期：2002-12-01 00:00:00

abstract：:A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...

journal_title：Journal of bacteriology

authors： Musser JM,Bemis DA,Ishikawa H,Selander RK

更新日期：1987-06-01 00:00:00

abstract：:Cystic fibrosis (CF) patients chronically infected with both Pseudomonas aeruginosa and Staphylococcus aureus have worse health outcomes than patients who are monoinfected with either P. aeruginosa or S. aureus We showed previously that mucoid strains of P. aeruginosa can coexist with S. aureusin vitro due to the tran...

journal_title：Journal of bacteriology

authors： Price CE,Brown DG,Limoli DH,Phelan VV,O'Toole GA

更新日期：2020-03-26 00:00:00

abstract：:Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed fro...

journal_title：Journal of bacteriology

authors： Brøndsted L,Atlung T

更新日期：1994-09-01 00:00:00

abstract：:Here, we present the high-quality draft genome sequence of the agar-degrading marine gammaproteobacterium Alteromonadaceae sp. strain G7, which was isolated from coastal seawater to be utilized as a bioresource for production of agar-derived biofuels. The 3.91-Mb genome contains a number of genes encoding algal polysa...

journal_title：Journal of bacteriology

authors： Kwak MJ,Song JY,Kim BK,Chi WJ,Kwon SK,Choi S,Chang YK,Hong SK,Kim JF

更新日期：2012-12-01 00:00:00

abstract：:An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...

journal_title：Journal of bacteriology

authors： Seeberg E,Steinum AL

更新日期：1980-03-01 00:00:00

abstract：:The Bacillus spo0H gene codes for sigma H, which, as part of the RNA polymerase holoenzyme E sigma H, is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus subtilis by using ...

journal_title：Journal of bacteriology

authors： Weir J,Predich M,Dubnau E,Nair G,Smith I

更新日期：1991-01-01 00:00:00

abstract：:The role of the carboxy terminus of the Escherichia coli cell division protein FtsA in bacterial division has been studied by making a series of short sequential deletions spanning from residue 394 to 420. Deletions as short as 5 residues destroy the biological function of the protein. Residue W415 is essential for th...

journal_title：Journal of bacteriology

authors： Yim L,Vandenbussche G,Mingorance J,Rueda S,Casanova M,Ruysschaert JM,Vicente M

更新日期：2000-11-01 00:00:00

abstract：:PacI and SpeI restriction maps were obtained for the two chromosomes of each of the six species of the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, and B. neotomae. Three complementary techniques were used: hybridization with the two replicons as probes, cross-hybridization of restriction fra...

journal_title：Journal of bacteriology

authors： Michaux-Charachon S,Bourg G,Jumas-Bilak E,Guigue-Talet P,Allardet-Servent A,O'Callaghan D,Ramuz M

更新日期：1997-05-01 00:00:00

abstract：:Rhizopines are selective growth substrates synthesized in nodules only by strains of rhizobia capable of their catabolism. We report the isolation and study of genes for the synthesis and catabolism of a new rhizopine, scyllo-inosamine (sIa), from alfalfa nodules induced by Rhizobium meliloti Rm220-3. This compound is...

journal_title：Journal of bacteriology

authors： Saint CP,Wexler M,Murphy PJ,Tempé J,Tate ME,Murphy PJ

更新日期：1993-08-01 00:00:00

abstract：:Filaments of a thermosensitive Bacillus megaterium mutant showed an altered macromolecular composition compared with salt-cured mutant cells and parental cells. Filaments contained more peptidoglycan, polyglucose, poly-beta-hydroxy-butyrate, and deoxyribonucleic acid per unit of protein. The ribonucleic acid-to-protei...

journal_title：Journal of bacteriology

authors： Yan LP,Hitchins AD

更新日期：1980-10-01 00:00:00

abstract：:Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal approximately 300 amino acids, while helicase motifs ...

journal_title：Journal of bacteriology

authors： Dostál L,Schildbach JF

更新日期：2010-07-01 00:00:00

abstract：:Group B streptococcus (GBS) pili may enhance colonization and infection by mediating bacterial adhesion to host cells, invasion across endothelial and epithelial barriers, and resistance to bacterial ingestion and killing by host phagocytes. However, it remains unclear how pilus expression is regulated and how modulat...

journal_title：Journal of bacteriology

authors： Jiang S,Park SE,Yadav P,Paoletti LC,Wessels MR

更新日期：2012-05-01 00:00:00

abstract：:The electron transport system of Neisseria gonorrhoeae was partially characterized by using spectrophotometric, spectroscopic, and oxygen consumption measurements. The effects of selected electron transport inhibitors (amytal, rotenone, 2-heptyl-4-hydroxyquinoline, antimycin A1, and potassium cyanide [KCN]) on electro...

journal_title：Journal of bacteriology

authors： Kenimer EA,Lapp DF

更新日期：1978-05-01 00:00:00

abstract：:Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine a...

journal_title：Journal of bacteriology

authors： PIZER LI

更新日期：1965-04-01 00:00:00

abstract：:Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cyste...

journal_title：Journal of bacteriology

authors： Mansilla MC,de Mendoza D

更新日期：1997-02-01 00:00:00

abstract：:Studies with cell-free protein preparations from a series of recombinant strains of Pseudomonas denitrificans demonstrated that precorrin-3 is converted into a further trimethylated intermediate, named precorrin-3B, along the pathway to coenzyme B12. It was then shown that the part of the pathway from precorrin-3 (cal...

journal_title：Journal of bacteriology

authors： Debussche L,Thibaut D,Cameron B,Crouzet J,Blanche F

更新日期：1993-11-01 00:00:00

abstract：:We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc₄-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation t...

journal_title：Journal of bacteriology

authors： Nguyen DH,Park JT,Shim JH,Tran PL,Oktavina EF,Nguyen TL,Lee SJ,Park CS,Li D,Park SH,Stapleton D,Lee JS,Park KH

更新日期：2014-06-01 00:00:00

abstract：:A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved ...

journal_title：Journal of bacteriology

authors： Savic DJ,Ferretti JJ

更新日期：2003-03-01 00:00:00

abstract：:In Bacillus subtilis the citM gene encodes the Mg(2+)-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region, including the cre sequence, to the beta-galactosidase reporter gene were constructed and integrated into the amyE site o...

journal_title：Journal of bacteriology

authors： Warner JB,Krom BP,Magni C,Konings WN,Lolkema JS

更新日期：2000-11-01 00:00:00

abstract：:The mode of synthesis and the regulation of fructose-1,6-bisphosphatase (Fbpase), a gluconeogenic enzyme, and phosphofructokinase (PFK), a glycolytic enzyme, were investigated in Saccharomyces cerevisiae after growth in the presence of different concentrations of glucose or various gluconeogenic carbon sources. The ac...

journal_title：Journal of bacteriology

authors： Foy JJ,Bhattacharjee JK

更新日期：1978-11-01 00:00:00

abstract：:We have sequenced the rightmost 1,700 base pairs of bacteriophage PRD1. This region encompasses the right early region and completes the sequence of all PRD1 early functions. We have also mapped the 5' initiation site of right early transcripts in vivo and in vitro. This has allowed us to assign gene XII to an open re...

journal_title：Journal of bacteriology

authors： Gerendasy D,Ito J

更新日期：1990-04-01 00:00:00

abstract：:The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...

journal_title：Journal of bacteriology

authors： Every D,Skerman TM

更新日期：1980-02-01 00:00:00

abstract：:When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two ge...

journal_title：Journal of bacteriology

authors： Kubitschek HE

更新日期：1971-02-01 00:00:00

abstract：:Cells of the Escherichia coli dnaE(Ts) dnaE74 and dnaE486 mutants die after 4 h of incubation at 40 degrees C in Luria-Bertani medium. Cell death is preceded by elongation, is inhibited by chloramphenicol, tetracycline, or rifampin, and is dependent on cell density. Cells survive at 40 degrees C when they are incubate...

journal_title：Journal of bacteriology

authors： Strauss B,Kelly K,Dincman T,Ekiert D,Biesieda T,Song R

更新日期：2004-04-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00