Abstract:
:In Bacillus subtilis, extracellular peptide signaling regulates several biological processes. Secreted Phr signaling peptides are imported into the cell and act intracellularly to antagonize the activity of regulators known as Rap proteins. B. subtilis encodes several Rap proteins and Phr peptides, and the processes regulated by many of these Rap proteins and Phr peptides are unknown. We used DNA microarrays to characterize the roles that several rap-phr signaling modules play in regulating gene expression. We found that rapK-phrK regulates the expression of a number of genes activated by the response regulator ComA. ComA activates expression of genes involved in competence development and the production of several secreted products. Two Phr peptides, PhrC and PhrF, were previously known to stimulate the activity of ComA. We assayed the roles that PhrC, PhrF, and PhrK play in regulating gene expression and found that these three peptides stimulate ComA-dependent gene expression to different levels and are all required for full expression of genes activated by ComA. The involvement of multiple Rap proteins and Phr peptides allows multiple physiological cues to be integrated into a regulatory network that modulates the timing and magnitude of the ComA response.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Auchtung JM,Lee CA,Grossman ADdoi
10.1128/JB.00300-06subject
Has Abstractpub_date
2006-07-01 00:00:00pages
5273-85issue
14eissn
0021-9193issn
1098-5530pii
188/14/5273journal_volume
188pub_type
杂志文章abstract::The nonstarter lactic acid bacterium Lactococcus raffinolactis is prevalent in a wide range of environments, such as the dairy environment, but little is known about this species. Here, we present the draft genome of Lactococcus raffinolactis strain 4877, isolated from a natural mesophilic dairy starter culture. ...
journal_title:Journal of bacteriology
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doi:10.1128/JB.135.3.1008-1014.1978
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
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doi:10.1128/JB.187.11.3678-3686.2005
更新日期:2005-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.182.9.2416-2421.2000
更新日期:2000-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.729-737.1973
更新日期:1973-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1656-1663.1967
更新日期:1967-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.1056-1058.1982
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
abstract::During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the plasmid-encoded relaxase. This strand is then unwound from its complement and transferred in the 5'-to-3' direction, with the 3' end likely extended by rolling-circle replication. The resulting, newly synthesized oriT must th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
abstract::Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1100-1109.1985
更新日期:1985-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.582-589.1974
更新日期:1974-05-01 00:00:00
abstract::The cysB region in Salmonella typhimurium regulates in a positive manner the noncontiguous structural genes for the enzymes responsible for sulfate reduction in cysteine biosynthesis. We treated three cysB mutants with chemical mutagens and selected 81 secondary mutants in which the inability to utilize sulfate was su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.94-101.1976
更新日期:1976-01-01 00:00:00