Abstract:
:Catheter-related bloodstream infections due to Staphylococcus aureus are of increasing clinical importance. The pathophysiological steps leading to colonization and infection, however, are still incompletely defined. We observed growth and detachment of S. aureus biofilms in an in vitro catheter-infection model by using time-lapse microscopy. Biofilm emboli were characterized by their size and their susceptibility for oxacillin. Biofilm dispersal was found to be a dynamic process in which clumps of a wide range of diameters detach. Large detached clumps were highly tolerant to oxacillin compared with exponential-phase planktonic cultures. Interestingly, the degree of antibiotic tolerance in stationary-phase planktonic cultures was equal to that in the large clumps. The mechanical disruption of large clumps reduced the minimal bactericidal concentration (MBC) by more than 1,000 times. The MBC for whole biofilm effluent, consisting of particles with an average number of 20 bacteria was 3.5 times higher than the MBC for planktonic cultures. We conclude that the antibiotic resistance of detached biofilm particles depends on the embolus size and could be attributed to nutrient-limited stationary-phase physiology of cells within the clumps. We hypothesize that the detachment of multicellular clumps may explain the high rate of symptomatic metastatic infections seen with S. aureus.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Fux CA,Wilson S,Stoodley Pdoi
10.1128/JB.186.14.4486-4491.2004keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
4486-91issue
14eissn
0021-9193issn
1098-5530pii
186/14/4486journal_volume
186pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/JB.127.2.904-916.1976
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doi:10.1128/JB.133.2.527-535.1978
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.160.1.427-429.1984
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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doi:10.1128/jb.173.22.7395-7400.1991
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journal_title:Journal of bacteriology
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doi:10.1128/JB.104.2.658-667.1970
更新日期:1970-11-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.148.1.210-219.1981
更新日期:1981-10-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.168.2.708-714.1986
更新日期:1986-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1987-10-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4939-4941.1988
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.21-26.1988
更新日期:1988-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5835-5837.1987
更新日期:1987-12-01 00:00:00