Abstract:
:Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination event usually occurred. Two plasmids, one of 2.6 kilobases and the other of 9.0 kilobases, were consistently isolated and shown by restriction enzyme analysis to be derived by recombination occurring in the pSA2100-pUB110 cointegrate. Analysis of the sequence of the junctions of the recombinant plasmids and of the crossover regions of the parental plasmids suggested that a reciprocal, conservative, intramolecular recombination event had occurred between short 18-base-pair homologous sequences that were oriented as direct repeats and bounded by regions of dyad symmetry. Evidence is presented that the above illegitimate recombination event is biased to occur intramolecularly and that randomly chosen direct repeats of either 22 or 29 base pairs are not sufficient to support recombination. The recombination event occurs in recA1, recB2, recD3, recE5, recL16, recM13, polA59, polA13, uvr-22, uvr-13, and stb mutants of B. subtilis and does not require that the competent state be established.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hahn J,Dubnau Ddoi
10.1128/JB.162.3.1014-1023.1985subject
Has Abstractpub_date
1985-06-01 00:00:00pages
1014-23issue
3eissn
0021-9193issn
1098-5530journal_volume
162pub_type
杂志文章abstract::Histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from Morganella morganii. All three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at pH 6.5, decarboxylated only histidine among the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.3963-3968.1987
更新日期:1987-09-01 00:00:00
abstract::The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies with F-pilin polypeptides in inner membrane preparations from various F mutant strains. It was known that JEL92 recognizes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1375-1383.1993
更新日期:1993-03-01 00:00:00
abstract::All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00630-13
更新日期:2013-10-01 00:00:00
abstract::Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.4875-4882.1994
更新日期:1994-08-01 00:00:00
abstract::Pentalenolactone (PL) irreversibly inactivates the enzyme glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)] (EC 1.2.1.12) and thus is a potent inhibitor of glycolysis in both procaryotic and eucaryotic cells. We showed that PL-producing strain Streptomyces ar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.930-936.1983
更新日期:1983-02-01 00:00:00
abstract::When ratios of the major polypeptides of the outer membrane isolated from cells of Escherichia coli B grown in minimal medium containing either a single amino acid or several amino acids were compared, no difference was observed. However, the ratio of these polypeptides in outer membrane material released into the med...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.2.1031-1034.1979
更新日期:1979-02-01 00:00:00
abstract::Nitrogen fixation occurs in two domains, Archaea and Bacteria. We have characterized a nif (nitrogen fixation) gene cluster in the methanogenic archaeon Methanococcus maripaludis. Sequence analysis revealed eight genes, six with sequence similarity to known nif genes and two with sequence similarity to glnB. The gene ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.6.1504-1511.1998
更新日期:1998-03-01 00:00:00
abstract::The F-plasmid-encoded TraI protein, also known as DNA helicase I, is a bifunctional protein required for conjugative DNA transfer. The enzyme catalyzes two distinct but functionally related reactions required for the DNA processing events associated with conjugation: the site- and strand-specific transesterification (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.697-706.2005
更新日期:2005-01-01 00:00:00
abstract::Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.23.6437-6447.2002
更新日期:2002-12-01 00:00:00
abstract::In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2876-2880.1987
更新日期:1987-06-01 00:00:00
abstract::Mevinolinic acid, the open acid form of mevinolin, which is a metabolite of Aspergillus terreus, has been shown to be a competitive inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (Alberts et al., Proc. Natl. Acad. Sci. U.S.A. 77:3957-3961, 1980). The biosynthesis of mevinolinic acid was studie...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.704-707.1985
更新日期:1985-05-01 00:00:00
abstract:UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00422-15
更新日期:2015-08-31 00:00:00
abstract::Many streptococcal pathogens require a polysaccharide capsule for survival in the host during systemic infection. The highly conserved CpsA protein is proposed to be a transcriptional regulator of capsule production in streptococci, although the regulatory mechanism is unknown. Hydropathy plots of CpsA predict an inte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01098-10
更新日期:2011-01-01 00:00:00
abstract::An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5904-5911.1994
更新日期:1994-10-01 00:00:00
abstract::The strict human pathogen Neisseria gonorrhoeae is exposed to oxidative damage during infection. N. gonorrhoeae has many defenses that have been demonstrated to counteract oxidative damage. However, recN is the only DNA repair and recombination gene upregulated in response to hydrogen peroxide (H(2)O(2)) by microarray...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00801-06
更新日期:2006-11-01 00:00:00
abstract::The two-component system SaeRS of Staphylococcus aureus is closely involved in the regulation of major virulence factors. However, little is known about the signals leading to saeRS activation. A total of four overlapping transcripts (T1 to T4) from three different transcription starting points are expressed in the sa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01927-07
更新日期:2008-05-01 00:00:00
abstract::Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.23.7346-7355.1999
更新日期:1999-12-01 00:00:00
abstract::Sinorhizobium meliloti, the nitrogen-fixing symbiont of alfalfa, has the ability to catabolize myo-, scyllo-, and D-chiro-inositol. Functional inositol catabolism (iol) genes are required for growth on these inositol isomers, and they play a role during plant-bacterium interactions. The inositol catabolism genes compr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05371-11
更新日期:2011-10-01 00:00:00
abstract::Integrons constitute a novel family of DNA elements which evolved by site-specific integration of discrete units between two conserved segments. On the In4 integron of Tn1696, a precisely inserted gene cassette of 1,549 bp conferring nonenzymatic chloramphenicol resistance (cmlA) is present between the streptomycin-sp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4493-4502.1991
更新日期:1991-07-01 00:00:00
abstract::A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total genomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5850-5859.1989
更新日期:1989-11-01 00:00:00
abstract::Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for norma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.917-919.1987
更新日期:1987-02-01 00:00:00
abstract::Halobacterium halobium swims with a polarly inserted motor-driven flagellar bundle. The swimming direction of the cell can be reserved by switching the rotational sense of the bundle. The switch is under the control of photoreceptor and chemoreceptor proteins that act through a branched signal chain. The swimming beha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.1971-1977.1991
更新日期:1991-03-01 00:00:00
abstract::Escherichia coli mutants lacking activities of all known cytosolic ATP-dependent proteases (Lon, ClpAP, ClpXP, and HslVU), due to double deletions [DeltahslVU and Delta(clpPX-lon)], cannot grow at low (30 degrees C) or very high (45 degrees C) temperatures, unlike those carrying either of the deletions. Such growth de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.12.3674-3680.1999
更新日期:1999-06-01 00:00:00
abstract::Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.316-322.1973
更新日期:1973-07-01 00:00:00
abstract::Mycobacterium xenopi is a slow-growing, thermophilic, water-related Mycobacterium species. Like other nontuberculous mycobacteria, M. xenopi more commonly infects humans with altered immune function, such as chronic obstructive pulmonary disease patients. It is considered clinically relevant in a significant proportio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00482-12
更新日期:2012-06-01 00:00:00
abstract::Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes. Class I mutants (RMS37 and RMS938) were defective in symbiotic C4-dicarboxylate transport and in nitrogen fixation. They were mutated in the structural g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5551-5560.1989
更新日期:1989-10-01 00:00:00
abstract::To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.23.7360-7368.1997
更新日期:1997-12-01 00:00:00
abstract::Silverman, Sidney J. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Lynn P. Elwell, and James F. Drawdy. Influence of route of infection and other factors on growth and distribution of Listeria monocytogenes in organs of mice. J. Bacteriol. 86:355-362. 1963.-The growth of Listeria monocytogenes in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.355-362.1963
更新日期:1963-09-01 00:00:00
abstract::Using physical and genetic data, we have demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasmid pRmeSU47a. This plasmid includes four loci involved in exopolysaccharide (exo) synthesis as well as two loci involved in thiamine biosyn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.66-72.1986
更新日期:1986-07-01 00:00:00
abstract::Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Detection and persistence of Vi antigen in tissues of actively immunized mice. J. Bacteriol. 89:776-781. 1965.-The presence, distribution, and persistence of Vi antigen in mouse tissue was determined by me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.776-781.1965
更新日期:1965-03-01 00:00:00