Membrane topology and DNA-binding ability of the Streptococcal CpsA protein.

abstract：:Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

journal_title：Journal of bacteriology

authors： Caffrey P,Owen P

更新日期：1989-07-01 00:00:00

abstract：:One of the phosphatidyl glycerophosphate phosphatase genes of Escherichia coli, pgpA, was cloned, and its DNA sequence was determined. Its 507-base-pair open reading frame was consistent with the 18,000-molecular-weight product identified by a maxicell experiment. Between its possible promoter and methionine initiatio...

journal_title：Journal of bacteriology

authors： Icho T

更新日期：1988-11-01 00:00:00

abstract：:Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be posttranslationally modified for full functionality; in enterobacteria, this is accomplished by two enzymes, namely, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a...

journal_title：Journal of bacteriology

authors： Klee SM,Sinn JP,Holmes AC,Lehman BL,Krawczyk T,Peter KA,McNellis TW

更新日期：2019-05-08 00:00:00

abstract：:Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...

journal_title：Journal of bacteriology

authors： Kanipes MI,Papp-Szabo E,Guerry P,Monteiro MA

更新日期：2006-05-01 00:00:00

abstract：:The Klebsiella pneumoniae nitrogen fixation (nif) mRNAs are unusually stable, with half-lives of 20 to 30 min under conditions favorable to nitrogen fixation (limiting nitrogen, anaerobiosis, temperatures of 30 degrees C). Addition of O2 or fixed nitrogen or temperature increases to 37 degrees C or more result in the ...

journal_title：Journal of bacteriology

authors： Simon HM,Gosink MM,Roberts GP

更新日期：1999-06-01 00:00:00

abstract：:The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. M...

journal_title：Journal of bacteriology

authors： Pomposiello PJ,Janes BK,Bender RA

更新日期：1998-02-01 00:00:00

abstract：:Prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by Treponema denticola. The gene, prtP, coding for the protease was recently cloned and sequenced (K. Ishihara, T. Miura, H. K. Kuramitsu, and K. Okuda, Infect. Immun. 64:5178-5186, 1996). In order to determine the ro...

journal_title：Journal of bacteriology

authors： Ishihara K,Kuramitsu HK,Miura T,Okuda K

更新日期：1998-08-01 00:00:00

abstract：:In the filamentous cyanobacterium Anabaena variabilis, specialized cells called heterocysts occur in a regular pattern along the filament and are the sites of nitrogen fixation. We used two different types of DNA-excess RNA hybridization techniques to estimate the number of genes expressed in recently differentiated, ...

journal_title：Journal of bacteriology

authors： Lynn ME,Bantle JA,Ownby JD

更新日期：1986-09-01 00:00:00

abstract：:Helicobacter pylori urease requires nickel ions in the enzyme active site for catalytic activity. Nickel ions must, therefore, be actively acquired by the bacterium. NixA (high-affinity nickel transport protein)-deficient mutants of H. pylori retain significant urease activity, suggesting the presence of alternate nic...

journal_title：Journal of bacteriology

authors： Hendricks JK,Mobley HL

更新日期：1997-09-01 00:00:00

abstract：:Sphingomonas wittichii RW1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. We used inverse PCR to amplify dxnB2, a gene encoding one of three meta-cleavage product (MCP) hydrolases identified in the organism that are homologues of BphD involved in biphenyl catabolism. Purified DxnB2 catalyz...

journal_title：Journal of bacteriology

authors： Seah SY,Ke J,Denis G,Horsman GP,Fortin PD,Whiting CJ,Eltis LD

更新日期：2007-06-01 00:00:00

abstract：:Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on ...

journal_title：Journal of bacteriology

authors： Tochikubo K

更新日期：1974-03-01 00:00:00

abstract：:Genetic circularity of the Pseudomonas aeruginosa PAO chromosome was demonstrated by a series of two- and three-factor crosses and double-selection experiments with Cma plasmids FP2, FP5, FP110, and R68.45. A range of additional markers, including catabolic markers, were located on the chromosome map. Plasmid FP2, kno...

journal_title：Journal of bacteriology

authors： Royle PL,Matsumoto H,Holloway BW

更新日期：1981-01-01 00:00:00

abstract：:Four regulated promoters that direct the transcription of genes (i.e., korA, tra, kilB, and korB) involved in the transfer of the Streptomyces plasmid pIJ101 were isolated following the in vitro fusion of plasmid DNA fragments to a promoterless gene encoding the S. lividans extracellular enzyme beta-galactosidase. Int...

journal_title：Journal of bacteriology

authors： Stein DS,Kendall KJ,Cohen SN

更新日期：1989-11-01 00:00:00

abstract：:Plasmids pRAS3.1 and pRAS3.2 are natural variants of the IncQ-2 plasmid family, that except for two differences, have identical plasmid backbones. Plasmid pRAS3.1 has four 22-bp iterons in its oriV region, while pRAS3.2 has only three 6-bp repeats and pRAS3.1 has five 6-bp repeats in the promoter region of the mobB-mo...

journal_title：Journal of bacteriology

authors： Loftie-Eaton W,Rawlings DE

更新日期：2010-12-01 00:00:00

abstract：:Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...

journal_title：Journal of bacteriology

authors： Moen MN,Knævelsrud I,Haugland GT,Grøsvik K,Birkeland NK,Klungland A,Bjelland S

更新日期：2011-09-01 00:00:00

abstract：:The nonstarter lactic acid bacterium Lactococcus raffinolactis is prevalent in a wide range of environments, such as the dairy environment, but little is known about this species. Here, we present the draft genome of Lactococcus raffinolactis strain 4877, isolated from a natural mesophilic dairy starter culture. ...

journal_title：Journal of bacteriology

authors： Meslier V,Loux V,Renault P

更新日期：2012-11-01 00:00:00

abstract：UNLABELLED:Multiple essential small GTPases are involved in the assembly of the ribosome or in the control of its activity. Among them, ObgE (CgtA) has been shown recently to act as a ribosome antiassociation factor that binds to ppGpp, a regulator whose best-known target is RNA polymerase. The present study was aimed ...

journal_title：Journal of bacteriology

authors： Maouche R,Burgos HL,My L,Viala JP,Gourse RL,Bouveret E

更新日期：2016-06-13 00:00:00

abstract：:Analysis of polypeptides in the crystalline delta-endotoxins from different Bacillus thuringiensis strains revealed two antigenically similar forms of the P2 protein which differed in molecular mass, peptide profile, and amino acid sequence. Purified preparations of the two forms displayed the characteristic dual toxi...

journal_title：Journal of bacteriology

authors： Nicholls CN,Ahmad W,Ellar DJ

更新日期：1989-09-01 00:00:00

abstract：:Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...

journal_title：Journal of bacteriology

authors： Parish T,Roberts G,Laval F,Schaeffer M,Daffé M,Duncan K

更新日期：2007-05-01 00:00:00

abstract：:Genomics research provides an unprecedented opportunity for us to probe into the pathogenicity and evolution of the world's most deadly pathogenic bacterium, Yersinia pestis, in minute detail. In our present work, extensive microarray analysis in conjunction with PCR validation revealed that there are considerable gen...

journal_title：Journal of bacteriology

authors： Zhou D,Han Y,Song Y,Tong Z,Wang J,Guo Z,Pei D,Pang X,Zhai J,Li M,Cui B,Qi Z,Jin L,Dai R,Du Z,Bao J,Zhang X,Yu J,Wang J,Huang P,Yang R

更新日期：2004-08-01 00:00:00

abstract：:The genes coding for two structurally different isocitrate dehydrogenase isozymes (IDH-I and IDH-II) of a psychrophilic bacterium, Vibrio sp. strain ABE-1, were cloned and sequenced. Open reading frames of the genes (icdI and icdII) are 1,248 and 2,229 bp in length, respectively. The amino acid sequences predicted fro...

journal_title：Journal of bacteriology

authors： Ishii A,Suzuki M,Sahara T,Takada Y,Sasaki S,Fukunaga N

更新日期：1993-11-01 00:00:00

abstract：:Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

journal_title：Journal of bacteriology

authors： Rocco JM,Churchward G

更新日期：2006-03-01 00:00:00

abstract：:The growth of strain araC(c)67, which produces the enzymes of the ara operon constitutively, is inhibited by the addition of ribitol. Isolation of strains resistant to ribitol yields mutants of either the araB or araC genes. A model to account for the inhibition by ribitol is discussed. ...

journal_title：Journal of bacteriology

authors： Katz L

更新日期：1970-05-01 00:00:00

abstract：:The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...

journal_title：Journal of bacteriology

authors： Jacobs NJ,Maclosky ER,Conti SF

更新日期：1967-01-01 00:00:00

abstract：:The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

journal_title：Journal of bacteriology

authors： Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

更新日期：2009-02-01 00:00:00

abstract：:Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many beta-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene ...

journal_title：Journal of bacteriology

authors： Doménech-Sánchez A,Hernández-Allés S,Martínez-Martínez L,Benedí VJ,Albertí S

更新日期：1999-05-01 00:00:00

abstract：:The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...

journal_title：Journal of bacteriology

authors： Maul RW,Sanders LH,Lim JB,Benitez R,Sutton MD

更新日期：2007-07-01 00:00:00

abstract：:SugE of Escherichia coli, first identified as a suppressor of groEL mutations but a member of the small multidrug resistance family, has not previously been shown to confer a drug resistance phenotype. We show that high-level expression of sugE leads to resistance to a subset of toxic quaternary ammonium compounds. ...

journal_title：Journal of bacteriology

authors： Chung YJ,Saier MH Jr

更新日期：2002-05-01 00:00:00

abstract：:Six mycoplasma strains, isolated under anaerobic conditions from the human oropharynx, were studied by biologic and serologic means. The strains produced nippled colonies with weak hemolytic activity for guinea pig erythrocytes on agar medium. In addition, the strains metabolized arginine with a concomitant alkaline s...

journal_title：Journal of bacteriology

authors： Fox H,Purcell RH,Chanock RM

更新日期：1969-04-01 00:00:00

abstract：:Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...

journal_title：Journal of bacteriology

authors： Helmstetter CE

更新日期：1974-10-01 00:00:00