Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1.

abstract：:We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...

journal_title：Journal of bacteriology

authors： Thoms B,Wackernagel W

更新日期：1987-04-01 00:00:00

abstract：:MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

journal_title：Journal of bacteriology

authors： Land AD,Winkler ME

更新日期：2011-08-01 00:00:00

abstract：:We have constructed strains of Pseudomonas aeruginosa with mutations in the algC gene, previously shown to encode the enzyme phosphomannomutase. The algC mutants of a serotype O5 strain (PAO1) and a serotype O3 strain (PAC1R) did not express lipopolysaccharide (LPS) O side chains or the A-band (common antigen) polysac...

journal_title：Journal of bacteriology

authors： Coyne MJ Jr,Russell KS,Coyle CL,Goldberg JB

更新日期：1994-06-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocy...

journal_title：Journal of bacteriology

authors： Ke S,Haselkorn R

更新日期：2013-01-01 00:00:00

abstract：:Different preparations of the methylreductase were tested in a simplified methylcoenzyme M methylreductase assay with artificial electron donors under a nitrogen atmosphere. ATP and Mg2+ stimulated the reaction. Tris(2,2'-bipyridine)ruthenium (II), chromous chloride, chromous acetate, titanium III citrate, 2,8-diamino...

journal_title：Journal of bacteriology

authors： Hartzell PL,Escalante-Semerena JC,Bobik TA,Wolfe RS

更新日期：1988-06-01 00:00:00

abstract：:ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...

journal_title：Journal of bacteriology

authors： Berlyn MB,Giles NH

更新日期：1969-07-01 00:00:00

abstract：:The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl ...

journal_title：Journal of bacteriology

authors： Maier E,Polleichtner G,Boeck B,Schinzel R,Benz R

更新日期：2001-01-01 00:00:00

abstract：:Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...

journal_title：Journal of bacteriology

authors： Heidinger S,Braun V,Pecoraro VL,Raymond KN

更新日期：1983-01-01 00:00:00

abstract：:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...

journal_title：Journal of bacteriology

authors： Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

更新日期：1990-11-01 00:00:00

abstract：:2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The purified enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 205 kDa by gel filtration chromatography. Thus, the homotetramer ...

journal_title：Journal of bacteriology

authors： Iwabuchi T,Harayama S

更新日期：1997-10-01 00:00:00

abstract：:Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, more than any described bacterium outside the order Bacteroidales. Here, we show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharid...

journal_title：Journal of bacteriology

authors： Chatzidaki-Livanis M,Coyne MJ,Roche-Hakansson H,Comstock LE

更新日期：2008-02-01 00:00:00

abstract：:When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

journal_title：Journal of bacteriology

authors： Yajko DM,Hegeman GD

更新日期：1971-12-01 00:00:00

abstract：:In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...

journal_title：Journal of bacteriology

authors： Mills LB,Stanbridge EJ,Sedwick WD,Korn D

更新日期：1977-11-01 00:00:00

abstract：:A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all ...

journal_title：Journal of bacteriology

authors： Plazinski J,Dart PJ,Rolfe BG

更新日期：1983-09-01 00:00:00

abstract：:A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinat...

journal_title：Journal of bacteriology

authors： Edwards DL,Belsole DM,Guzik HJ,Unger BW

更新日期：1979-02-01 00:00:00

abstract：:Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infe...

journal_title：Journal of bacteriology

authors： Akama T,Suzuki K,Tanigawa K,Kawashima A,Wu H,Nakata N,Osana Y,Sakakibara Y,Ishii N

更新日期：2009-05-01 00:00:00

abstract：:CRISPR-Cas systems are prokaryotic adaptive immune systems that facilitate protection of bacteria and archaea against infection by external mobile genetic elements. The model pathogen Francisella novicida encodes a CRISPR-Cas12a (FnoCas12a) system and a CRISPR-Cas9 (FnoCas9) system, the latter of which has an addition...

journal_title：Journal of bacteriology

authors： Ratner HK,Weiss DS

更新日期：2020-05-27 00:00:00

abstract：:Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addre...

journal_title：Journal of bacteriology

authors： Armbruster CE,Hodges SA,Mobley HL

更新日期：2013-03-01 00:00:00

abstract：:A new carboxypeptidase (carboxypeptidase S) was found in a Saccharomyces cerevisiae strain lacking carboxypeptidase Y (D. H. Wolf and U. Weiser, Eur. J. Biochem. 73:553-556, 1977). Mutants devoid of carboxypeptidase S activity were isolated from a mutant strain that was also deficient in carboxypeptidase Y. Four mutan...

journal_title：Journal of bacteriology

authors： Wolf DH,Ehmann C

更新日期：1981-08-01 00:00:00

abstract：:Transcription of the virG gene of Agrobacterium tumefaciens was previously shown to be expressed from two tandem promoters and to be responsive to three stimuli: plant-released phenolic compounds, phosphate starvation, and acidic media. In this report, I describe a set of deletions and other alterations of the 5' end ...

journal_title：Journal of bacteriology

authors： Winans SC

更新日期：1990-05-01 00:00:00

abstract：:The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

journal_title：Journal of bacteriology

authors： Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

更新日期：1975-11-01 00:00:00

abstract：:Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...

journal_title：Journal of bacteriology

authors： LeVier K,Walker GC

更新日期：2001-11-01 00:00:00

abstract：:Biberstein, Ernst L. (University of California, Davis) and Patricia D. Spencer. Oxidative metabolism of Haemophilus species grown at different levels of hemin supplementation. J. Bacteriol. 84:916-920. 1962.-Haemophilus strains were propagated on hemin-deficient and hemin-supplemented media and tested on various subst...

journal_title：Journal of bacteriology

authors： BIBERSTEIN EL,SPENCER PD

更新日期：1962-11-01 00:00:00

abstract：:Soluble c-type cytochromes are central to metabolism of C1 compounds in methylotrophic bacteria. In order to characterize the role of c-type cytochromes in methane-utilizing bacteria (methanotrophs), we have purified four different cytochromes, cytochromes c-554, c-553, c-552, and c-551, from the marine methanotroph M...

journal_title：Journal of bacteriology

authors： DiSpirito AA,Lipscomb JD,Lidstrom ME

更新日期：1990-09-01 00:00:00

abstract：:Eisenberg, M. A. (Columbia University, New York, N.Y.). Biotin biosynthesis. I. Biotin yields and biotin vitamers in cultures of Phycomyces blakesleeanus. J. Bacteriol. 86:673-680. 1963.-The addition of pimelic acid to a well-aerated medium resulted in a 10- to 12-fold increase in the biotin production of Phycomyces b...

journal_title：Journal of bacteriology

authors： EISENBERG MA

更新日期：1963-10-01 00:00:00

abstract：:The PRO1 gene of Saccharomyces cerevisiae encodes the 428-amino-acid protein gamma-glutamyl kinase (ATP:L-glutamate 5-phosphotransferase, EC 2.7.2.11), which catalyzes the first step in proline biosynthesis. Amino acid sequence comparison revealed significant homology between the yeast and Escherichia coli gamma-gluta...

journal_title：Journal of bacteriology

authors： Li W,Brandriss MC

更新日期：1992-06-01 00:00:00

abstract：:We present the genomic sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H (ATCC 43290), a clinical isolate from the Colorado Department of Health, Denver, CO. This is the first example of a genome sequence of L. pneumophila from a serogroup other than serogroup 1. We highlight the simil...

journal_title：Journal of bacteriology

authors： Amaro F,Gilbert JA,Owens S,Trimble W,Shuman HA

更新日期：2012-03-01 00:00:00

abstract：:Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...

journal_title：Journal of bacteriology

authors： Loewen PC,Switala J

更新日期：1987-12-01 00:00:00

abstract：:Entomopathogenic bacteria of the genus Photorhabdus harbor a type III secretion system. This system was probably acquired prior to the separation of the species within this genus. Furthermore, the core components of the secretion machinery are highly conserved but the predicted effectors differ between Photorhabdus lu...

journal_title：Journal of bacteriology

authors： Brugirard-Ricaud K,Givaudan A,Parkhill J,Boemare N,Kunst F,Zumbihl R,Duchaud E

更新日期：2004-07-01 00:00:00

abstract：:The effect of sublethal, midlethal, and lethal doses of total-body X irradiation on the fecal microbial population of the rat was studied. No changes were observed in animals receiving sublethal doses of X ray. Midlethal and lethal doses produced an increase in the numbers of fecal coliforms, staphylococci, streptococ...

journal_title：Journal of bacteriology

authors： Klainer AS,Gorbach S,Weinstein L

更新日期：1967-08-01 00:00:00