Identification of the outer membrane porin of Thermus thermophilus HB8: the channel-forming complex has an unusually high molecular mass and an extremely large single-channel conductance.

Abstract:

:The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl sulfate (SDS)-polyacylamide gel electrophoresis. It has a high molecular mass of 185 kDa, and its channel-forming ability resists boiling in SDS for 10 min.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Maier E,Polleichtner G,Boeck B,Schinzel R,Benz R

doi

10.1128/JB.183.2.800-803.2001

keywords:

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

800-3

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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