Abstract:
:The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes, respectively. We now report the crystal structure of E. coli pyridoxal kinase 1 (ePL kinase 1), encoded by a pdxK gene, and an isoform of ePL kinase 2. The structures were determined in the unliganded and binary complexes with either MgATP or pyridoxal to 2.1-, 2.6-, and 3.2-A resolutions, respectively. The active site of ePL kinase 1 does not show significant conformational change upon binding of either pyridoxal or MgATP. Like sheep PL kinase, ePL kinase 1 exhibits a sequential random mechanism. Unlike sheep pyridoxal kinase, ePL kinase 1 may not tolerate wide variation in the size and chemical nature of the 4' substituent on the substrate. This is the result of differences in a key residue at position 59 on a loop (loop II) that partially forms the active site. Residue 59, which is His in ePL kinase 1, interacts with the formyl group at C-4' of pyridoxal and may also determine if residues from another loop (loop I) can fill the active site in the absence of the substrate. Both loop I and loop II are suggested to play significant roles in the functions of PL kinases.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Safo MK,Musayev FN,di Salvo ML,Hunt S,Claude JB,Schirch Vdoi
10.1128/JB.00122-06subject
Has Abstractpub_date
2006-06-01 00:00:00pages
4542-52issue
12eissn
0021-9193issn
1098-5530pii
188/12/4542journal_volume
188pub_type
杂志文章abstract::SugE of Escherichia coli, first identified as a suppressor of groEL mutations but a member of the small multidrug resistance family, has not previously been shown to confer a drug resistance phenotype. We show that high-level expression of sugE leads to resistance to a subset of toxic quaternary ammonium compounds. ...
journal_title:Journal of bacteriology
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doi:10.1128/jb.184.9.2543-2545.2002
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abstract::Salmonella enteritidis was injected intravenously, intraperitoneally, or subcutaneously into specific pathogen-free mice. The number of organisms in the blood, liver, spleen, peritoneal cavity, and draining inguinal lymph node was determined by daily enumeration. Opsonization of the organism with hyperimmune serum inc...
journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/jb.182.10.2732-2740.2000
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abstract::We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1307-1314.1976
更新日期:1976-09-01 00:00:00
abstract::Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.85-90.1978
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.953-962.1973
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2724-2732.2001
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00909-09
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1003-1009.1987
更新日期:1987-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.122-129.1978
更新日期:1978-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.225-231.1975
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.4.1003-1009.2002
更新日期:2002-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.22.6658-6665.2003
更新日期:2003-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1328-1333.1969
更新日期:1969-03-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.95.2.426-432.1968
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.20-26.1962
更新日期:1962-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00349-15
更新日期:2015-10-01 00:00:00
abstract::A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.544-547.1997
更新日期:1997-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.562-563.1975
更新日期:1975-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.440-446.1979
更新日期:1979-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1035-1042.1975
更新日期:1975-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3397-3403.1997
更新日期:1997-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
abstract::In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3429-3438.1992
更新日期:1992-06-01 00:00:00