Abstract:
:A procedure is described that uses an indicator plasmid (pSC201) to identify cells in a bacterial population that have been co-transformed with a second plasmid lacking detectable phenotypic properties. Under appropriate conditions of indirect selection, between 50 and 85% of transformants carrying the indicator plasmid also contain the nonselected plasmid. A temperature-sensitive mutation in the replication functions of the indicator plasmid enables its elimination from doubly transformed bacteria. Using this procedure, we have isolated bacteria that carry only the small cryptic plasmid. P15A, of the Escherichia coli strain 15. This genetic element, which contains only 2,300 nucleotide pairs, is thus capable of functioning as a replicon independently of the two larger plasmids normally associated with it in E. coli 15 strains (Ikeda, Inuzuka, and Tomizawa, 1970).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kretschmer FJ,Chang AC,Cohen SNdoi
10.1128/JB.124.1.225-231.1975keywords:
subject
Has Abstractpub_date
1975-10-01 00:00:00pages
225-31issue
1eissn
0021-9193issn
1098-5530journal_volume
124pub_type
杂志文章abstract::The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.973-976.1982
更新日期:1982-05-01 00:00:00
abstract::Mycoplasma pneumoniae harvested from media which had become acid lost the ability both to induce formation of tetrazolium reduction inhibition antibody and to act as antigens in immunodiffusion against human convalescent-phase sera. Incorporation of N-tris(hydroxymethyl)-2-aminoethane sulfonic acid and N-2-hydroxyethy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.612-619.1969
更新日期:1969-02-01 00:00:00
abstract::We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4488-4500.1995
更新日期:1995-08-01 00:00:00
abstract::A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.11.3197-3203.2000
更新日期:2000-06-01 00:00:00
abstract::The cysB region in Salmonella typhimurium regulates in a positive manner the noncontiguous structural genes for the enzymes responsible for sulfate reduction in cysteine biosynthesis. We treated three cysB mutants with chemical mutagens and selected 81 secondary mutants in which the inability to utilize sulfate was su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.94-101.1976
更新日期:1976-01-01 00:00:00
abstract::CRISPR-Cas systems are prokaryotic adaptive immune systems that facilitate protection of bacteria and archaea against infection by external mobile genetic elements. The model pathogen Francisella novicida encodes a CRISPR-Cas12a (FnoCas12a) system and a CRISPR-Cas9 (FnoCas9) system, the latter of which has an addition...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00670-19
更新日期:2020-05-27 00:00:00
abstract::Poly-beta-hydroxybutyrate (PHB) synthesis in a cyanobacterium, Synechococcus sp. strain MA19, is controlled at the enzyme level and is dependent on the C/N balance in the culture medium. The control involves at least two enzymes. The first enzyme is PHB synthase. Little PHB synthase activity was detected in crude extr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.16.5009-5013.1997
更新日期:1997-08-01 00:00:00
abstract::A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.1.11-19.1995
更新日期:1995-01-01 00:00:00
abstract::Bacterial cell division is a complex process that relies on a multiprotein complex composed of a core of widely conserved and generally essential proteins and on accessory proteins that vary in number and identity in different bacteria. The assembly of this complex and, particularly, the initiation of constriction are...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00811-16
更新日期:2017-03-28 00:00:00
abstract::Mutants of Escherichia coli were isolated by their resistance to the bacteriocidal effects of the membrane-active drugs polymyxin B, levallorphan, and tetracaine. The mutants were examined for additional changes in cellular physiology evoked by the lesions; many polymyxin-resistant strains had a concomitant increased ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.2.961-972.1976
更新日期:1976-08-01 00:00:00
abstract::Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.18.6561-6564.2005
更新日期:2005-09-01 00:00:00
abstract::The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was clon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5661-5671.2004
更新日期:2004-09-01 00:00:00
abstract::Davies, Helen C. (University of Pennsylvania, Philadelphia), Fred Karush, and Joanne H. Rudd. Effect of amino acids on steady-state growth of a group A hemolytic streptococcus. J. Bacteriol. 89:421-427. 1965.-A study has been made of amino acid utilization by a strain of type 4, group A streptococcus growing under ste...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1965-02-01 00:00:00
abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02222-14
更新日期:2014-12-01 00:00:00
abstract::E2 (dihydrolipoamide acetyltransferase) and E3 (dihydrolipoamide dehydrogenase) of the Clostridium magnum acetoin dehydrogenase enzyme system were copurified in a three-step procedure from acetoin-grown cells. The denatured E2-E3 preparation comprised two polypeptides with M(r)s of 49,000 and 67,000, respectively. Mic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3614-3630.1994
更新日期:1994-06-01 00:00:00
abstract::In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01063-08
更新日期:2009-01-01 00:00:00
abstract:UNLABELLED:Neisseria meningitidis, an exclusively human pathogen and the leading cause of bacterial meningitis, must adapt to different host niches during human infection. N. meningitidis can utilize a restricted range of carbon sources, including lactate, glucose, and pyruvate, whose concentrations vary in host niches...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00659-15
更新日期:2015-12-07 00:00:00
abstract::Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.108-121.1976
更新日期:1976-04-01 00:00:00
abstract::Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.508-512.2000
更新日期:2000-01-01 00:00:00
abstract::Biofilm formation is a key factor in Vibrio cholerae environmental survival and host colonization. Production of biofilm enables V. cholerae to survive and persist in aquatic environments and aids in the passage through the gastric acid barrier to allow access to the small intestine. The genes involved in biofilm form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00014-12
更新日期:2012-06-01 00:00:00
abstract::Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1621-1623.1975
更新日期:1975-12-01 00:00:00
abstract::The nucleotide sequence of a 7.3-kilobase-pair fragment of DNA encoding a hemolytic activity from Serratia marcescens was determined. Two large open reading frames were identified, designated shlA (Serratia hemolysin) and shlB, capable of encoding polypeptides of 165, 056 and 61,897 molecular weight, respectively. Bot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.3177-3188.1988
更新日期:1988-07-01 00:00:00
abstract::The draft genome sequence (4,398,155 bp, with 65.35% G+C content) of Halobiforma lacisalsi AJ5, an extremely halophilic archaeon isolated from a salt lake, is reported here. This is the first genome report for a species of the Halobiforma genus. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06282-11
更新日期:2011-12-01 00:00:00
abstract::Sguros, Peter L. (University of Miami, Miami, Fla.), and Jacqueline Simms. Role of marine fungi in the biochemistry of the oceans. IV. Growth responses to seawater inorganic macroconstituents. J. Bacteriol. 88:346-355. 1964.-The filamentous ascomycete Halosphaeria mediosetigera and the deuteromycete Culcitalna achrasp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.2.346-355.1964
更新日期:1964-08-01 00:00:00
abstract::Although nitrate stimulated the capacity of Clostridium thermoautotrophicum and Clostridium thermoaceticum to oxidize (utilize) substrates under heterotrophic conditions, it inhibited autotrophic H2-CO2-dependent growth. Under basal medium conditions, nitrate was also inhibitory to the use of one-carbon substrates (i....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4597-4603.1996
更新日期:1996-08-01 00:00:00
abstract::Pathogenic Yersinia species employ a type III secretion system (TTSS) to target antihost factors, Yop proteins, into eukaryotic cells. The secretion machinery is constituted of ca. 20 Ysc proteins, nine of which show significant homology to components of the flagellar TTSS. A key event in flagellar assembly is the swi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.16.4500-4509.2002
更新日期:2002-08-01 00:00:00
abstract::An earlier study of the regulation of glutamate synthase (GOGAT) in Bacillus subtilis (Deshpande et al., Bichem. Biophys. Res. Commun. 95:55--60, 1980) revealed an inverse relationship between the specific activity of this essential ammonia-assimilatory enzyme and the intracellular pool of glutamine: GOGAT activity de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.768-774.1981
更新日期:1981-02-01 00:00:00
abstract::In the yeast Saccharomyces cerevisiae, IRE1 encodes a bifunctional protein with transmembrane kinase and endoribonuclease activities. HAC1 encodes a transcription factor which has a basic leucine zipper domain. Both gene products play a crucial role in the unfolded protein response. Mutants in which one of these genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.17.4985-4993.2001
更新日期:2001-09-01 00:00:00
abstract::The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.7.2528-2532.2006
更新日期:2006-04-01 00:00:00
abstract::Over most of the range of salt concentrations in which the moderately halophilic bacterium Vibrio costicola could grow, the sum of the cell-associated Na+ + K+ ions was at least as high as in the external medium. This is in contrast to other moderate halophiles, which have substantially lower internal than external sa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.2.698-703.1977
更新日期:1977-05-01 00:00:00