Effect of glutamine on enzymes of nitrogen metabolism in Bacillus subtilis.

abstract：:To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...

journal_title：Journal of bacteriology

authors： Duret S,Danet JL,Garnier M,Renaudin J

更新日期：1999-12-01 00:00:00

abstract：:Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

journal_title：Journal of bacteriology

authors： Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

更新日期：2014-04-01 00:00:00

abstract：:Fatty acid desaturation catalyzed by fatty acid desaturases requires molecular oxygen (O(2)). Saccharomyces cerevisiae cells derepress expression of OLE1 encoding Delta9 fatty acid desaturase under hypoxic conditions to allow more-efficient use of limited O(2). It has been proposed that aerobic conditions lead to repr...

journal_title：Journal of bacteriology

authors： Nakagawa Y,Sugioka S,Kaneko Y,Harashima S

更新日期：2001-01-01 00:00:00

abstract：:The thiol-containing tripeptide glutathione is an important cellular constituent of many eukaryotic and prokaryotic cells. In addition to its disulfide reductase activity, glutathione is known to protect cells from many forms of physiological stress. This report represents the first investigation into the role of glut...

journal_title：Journal of bacteriology

authors： Potter AJ,Trappetti C,Paton JC

更新日期：2012-11-01 00:00:00

abstract：:A globomycin-resistant mutant of Escherichia coli was found to produce a precursor of the major outer membrane lipoprotein (prolipoprotein), in which the glycine residue at position 14 within the signal peptide was replaced by an aspartic acid residue. The same mutation has been reported by Lin et al. (Proc. Natl. Aca...

journal_title：Journal of bacteriology

authors： Lee N,Yamagata H,Inouye M

更新日期：1983-07-01 00:00:00

abstract：:Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...

journal_title：Journal of bacteriology

authors： Jasper P

更新日期：1978-03-01 00:00:00

abstract：:The SOS response in bacteria includes a global transcriptional response to DNA damage. DNA damage is sensed by the highly conserved recombination protein RecA, which facilitates inactivation of the transcriptional repressor LexA. Inactivation of LexA causes induction (derepression) of genes of the LexA regulon, many o...

journal_title：Journal of bacteriology

authors： Au N,Kuester-Schoeck E,Mandava V,Bothwell LE,Canny SP,Chachu K,Colavito SA,Fuller SN,Groban ES,Hensley LA,O'Brien TC,Shah A,Tierney JT,Tomm LL,O'Gara TM,Goranov AI,Grossman AD,Lovett CM

更新日期：2005-11-01 00:00:00

abstract：:2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The purified enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 205 kDa by gel filtration chromatography. Thus, the homotetramer ...

journal_title：Journal of bacteriology

authors： Iwabuchi T,Harayama S

更新日期：1997-10-01 00:00:00

abstract：:For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...

journal_title：Journal of bacteriology

authors： Das S,Kellermann E,Hollenberg CP

更新日期：1984-06-01 00:00:00

abstract：:The broad-host-range plasmid RK2 is capable of replication and stable maintenance within a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation protein TrfA, which binds to the host initiation protein, DnaA, at the plasmid origin of replication (oriV). There are two versions of ...

journal_title：Journal of bacteriology

authors： Kongsuwan K,Josh P,Picault MJ,Wijffels G,Dalrymple B

更新日期：2006-08-01 00:00:00

abstract：:Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble alpha-glucosidase (maltase) and flanking sequences from Sulfolobus solfa...

journal_title：Journal of bacteriology

authors： Rolfsmeier M,Haseltine C,Bini E,Clark A,Blum P

更新日期：1998-03-01 00:00:00

abstract：:Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular enterotoxin concentration roughly paralleled but followed by 2.5 to 5 hr the increase in number of heat-resistant spores. The increase ...

journal_title：Journal of bacteriology

authors： Duncan CL

更新日期：1973-02-01 00:00:00

abstract：:Bartnicki-Garcia, S. (Rutgers, The State University, New Brunswick, N.J.) and Walter J. Nickerson. Nutrition, growth, and morphogenesis of Mucor rouxii. J. Bacteriol. 84:841-858. 1962.-Mucor rouxii was grown under three different atmospheres of incubation: air, N(2), and CO(2) in parallel cultures. The atmosphere of i...

journal_title：Journal of bacteriology

authors： BARTNICKI-GARCIA S,NICKERSON WJ

更新日期：1962-10-01 00:00:00

abstract：:The colicinogenic B factor, transferred from Escherichia coli strain K77 (and termed ColB2-K77 or ColB2) to an E. coli K12 F(-) strain, is capable of promoting its own transfer to other K12 F(-) strains at a low rate (from LFC cultures) which can be increased under special conditions (HFC cultures). LFC cultures of K1...

journal_title：Journal of bacteriology

authors： Hausmann C,Clowes RC

更新日期：1971-09-01 00:00:00

abstract：:In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was...

journal_title：Journal of bacteriology

authors： Messenger AJ,Ratledge C

更新日期：1982-01-01 00:00:00

abstract：:Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the heterodimeric, membrane-bound hydrogenase (MBH) are located adjacent to a series of eight accessory genes (hoxZ, hoxM, h...

journal_title：Journal of bacteriology

authors： Bernhard M,Schwartz E,Rietdorf J,Friedrich B

更新日期：1996-08-01 00:00:00

abstract：:Gasdorf, Helen J. (Northern Regional Research Laboratory, Peoria, Ill.), R. G. Benedict, M. C. Cadmus, R. F. Anderson, and R. W. Jackson. Polymer-producing species of Arthrobacter. J. Bacteriol. 90:147-150.1965.-Two slime-producing microorganisms, designated as NRRL B-1973 and NRRL B-1797, were isolated from a Guatema...

journal_title：Journal of bacteriology

authors： Gasdorf HJ,Benedict RG,Cadmus MC,Anderson RF,Jackson RW

更新日期：1965-07-01 00:00:00

abstract：:Growth of Schizosaccharomyces pombe in batch cultures in a commercial defined medium was found to be normal until the last generation before the stationary phase. Approximately one-half the progeny of the last division lysed. Lysis occurred at breaks in the cell wall at cell-plates whose fission was eccentric. Normal ...

journal_title：Journal of bacteriology

authors： Johnson BF

更新日期：1967-07-01 00:00:00

abstract：:Glycine betaine is an effective osmoprotectant for Bacillus subtilis. Its import into osmotically stressed cells led to the buildup of large pools, whose size was sensitively determined by the degree of the osmotic stress imposed. The amassing of glycine betaine caused repression of the formation of an osmostress-adap...

journal_title：Journal of bacteriology

authors： Hoffmann T,Wensing A,Brosius M,Steil L,Völker U,Bremer E

更新日期：2013-02-01 00:00:00

abstract：:The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine prot...

journal_title：Journal of bacteriology

authors： Aizawa SI,Dean GE,Jones CJ,Macnab RM,Yamaguchi S

更新日期：1985-03-01 00:00:00

abstract：:Gene amplifications have been detected as a transitory phenomenon in bacterial cultures. They are predicted to contribute to rapid adaptation by simultaneously increasing the expression of genes clustered on the chromosome. However, genome amplifications have rarely been described in natural isolates. Through DNA arra...

journal_title：Journal of bacteriology

authors： Brochet M,Couvé E,Zouine M,Poyart C,Glaser P

更新日期：2008-01-01 00:00:00

abstract：:The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

journal_title：Journal of bacteriology

authors： Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

更新日期：2009-02-01 00:00:00

abstract：:Acid hydrolysates of 140 strains, representing 11 species of the genus Bacteroides, were analyzed by capillary gas-liquid chromatography for total cellular fatty acid. All samples contained components which appeared to be hydroxy fatty acid. The relative amount and chain length distribution of the hydroxy fatty acids,...

journal_title：Journal of bacteriology

authors： Mayberry WR

更新日期：1980-08-01 00:00:00

abstract：:Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...

journal_title：Journal of bacteriology

authors： Korshunov S,Imlay JA

更新日期：2006-09-01 00:00:00

abstract：:Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of th...

journal_title：Journal of bacteriology

authors： Wallace BJ,Pittard J

更新日期：1967-01-01 00:00:00

abstract：:We present the genomic sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H (ATCC 43290), a clinical isolate from the Colorado Department of Health, Denver, CO. This is the first example of a genome sequence of L. pneumophila from a serogroup other than serogroup 1. We highlight the simil...

journal_title：Journal of bacteriology

authors： Amaro F,Gilbert JA,Owens S,Trimble W,Shuman HA

更新日期：2012-03-01 00:00:00

abstract：:The iota(a) component (i(a)) of Clostridium perfringens ADP ribosylates nonmuscle beta/gamma actin and skeletal muscle alpha-actin. Replacement of Arg-295 in i(a) with alanine led to a complete loss of NAD(+)-glycohydrolase (NADase) and ADP-ribosyltransferase (ARTase); that of the residue with lysine caused a drastic ...

journal_title：Journal of bacteriology

authors： Nagahama M,Sakaguchi Y,Kobayashi K,Ochi S,Sakurai J

更新日期：2000-04-01 00:00:00

abstract：:top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

journal_title：Journal of bacteriology

authors： Pruss GJ,Drlica K

更新日期：1985-11-01 00:00:00

abstract：:The transport system for glycylglycine in Escherichia coli behaves like a shock-sensitive transport system. The initial rate of transport is reduced 85% by subjecting whole cells to osmotic shock, and glycylglycine is not transported by membrane vesicles. The energetics of transport was studied with strain ML 308-225 ...

journal_title：Journal of bacteriology

authors： Cowell JL

更新日期：1974-10-01 00:00:00

abstract：:The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

journal_title：Journal of bacteriology

authors： Kim SW,Kim CY,Benisek WF,Choi KY

更新日期：1994-11-01 00:00:00